You are here: Home > Products >

Mammalian Stable Cell Line Development Services

Stable cell lines are widely used in recombinant protein and antibody production, drug screening, gene functional studies and other applications. They can grow continuously over a prolonged period of time and stably carry a genetic modification or express a transgene without significant changes in expression levels.

GeneCopoeia offers state-of-the art services for establishing stable cell lines for protein overexpression, gene knockdown or genome editing that meet your specific research needs.

 

Protein Overexpression

Stable cell lines with ORF clones

Overexpression of proteins is critical for many applications, including large-scale antibody production, live cell imaging for localization experiments, and purification for structure-function studies. Traditional overexpression in bacteria often renders eukaryotic proteins insoluble, due to misfolding and/or improper post-translational modification. Therefore, it is highly desirable to be able to express eukaryotic proteins in a physiologically relevant system.

GeneCopoeia offers the generation of stable cell lines that can overexpress virtually any protein of interest in your cell line of choice. Customers can choose to have their own expression cassette integrated into the genome, or from among more than 53,000 human and 32,000 mouse ORFs from GeneCopoeia’s extensive ORF clone collection.

 

Applications

  • Monoclonal antibody production
  • Protein production for biochemical assays
  • Protein production for crystal or NMR structural determination
  • Fusion tagging for live cell imaging
  • Fusion tagging for pulldown/immunoprecipitation
  • Drug target analysis

 

Advantages

  • Largest collection of sequence-verified and expression-ready ORF clones/lentiviral particles reduces turnaround time and ensures high quality.
  • Choice of promoters including inducible promoters for toxic gene expression
  • MAR option for increased open-chromatin site integration
  • Safe harbor knockin ORF clones available for designated safe site integration and physiological levels of transgene expression
  • DHFR or GS gene amplification available

 

To Order

To request a quote for our stable cell line services, please fill out the inquiry form.

Request a Quote

 

Related Products

Gene Knockdown

Stable cell lines expressing shRNAs

Down-regulating gene expression is of fundamental importance to the study of gene function. In higher eukaryotes, which have been historically difficult to manipulate for making complete genetic knock outs, researchers have used RNA interference (RNAi), which reduces gene expression post-transcriptionally without making permanent changes in the chromosome, irrespective of the chromatin structure or DNA methylation state of the target locus. RNAi causes “knock down”, reducing but not completely eliminating gene expression. Knock down can be advantageous over knock out in applications where complete ablation of gene expression is lethal, or in cases where one wants downregulation to be conditional. In contrast to short interfering RNAs (siRNAs), which knock genes down transiently, short hairpin RNAs (shRNAs) can be integrated into the chromosome to downregulate gene expression stably or conditionally.

GeneCopoeia offers the generation of cell lines stably expressing shRNAs targeting a gene of interest. We will design your shRNAs and integrate them into virtually any cell line you choose.

 

Applications

  • Determination of gene function in cells
  • Expression profiling to identify candidate downstream genes in a network or pathway
  • Drug target validation

 

Advantages

  • Largest collection of sequence-verified and expression-ready shRNA clones/lentiviral particles reduces turnaround time and ensures high quality.
  • Inducible promoters available for toxic gene expression

 

To Order

To request a quote for TALEN or CRISPR based stable cell line services, please fill out the inquiry form.

Request a Quote

 

Related Products

Genome Editing

Stable cell lines constructed with CRISPR or TALEN

Genome editing-the ability to make specific changes at targeted genomic sites in complex organisms-is of fundamental importance in biology and medicine. Over the past decade, researchers have developed chimeric DNA binding proteins complexed with nucleases to stimulate double strand breaks at defined genomic loci, allowing the ability to insert, delete, and replace genetic information at will. While zinc finger nucleases (ZFNs) stimulated tremendous development in the field, they suffer from significant limitations in design capacity and specificity. Recently, bacterial DNA binding proteins, the Transcription Activator Like Effectors (TALE) and the Clustered, Regularly Interspersed, Short Palindromic Repeats (CRISPR) systems have gained favor as genome editing tools.

Along with TALE- and CRISPR-based plasmid design and construction services,GeneCopoeia offers the generation of stable cell lines carrying specific, targeted modifications created with either CRISPR or TALEN. We will design the CRISPR/TALEN target site, donor constructs used for homologous recombination-mediated applications, transfect your cell line of choice, and isolate single or double allele-modified clones. Our stable cell line service can also be used with our human and mouse Safe Harbor Integration system and knock in ORF clones.

 

Applications

  • Gene knock out
  • Introduction of point mutations or defined insertions/deletions
  • Correction of disease mutations back to wild type
  • Transgene knock in at a customer-specified locus or at human and mouse Safe Harbor sites
  • Gene tagging
  • Promoter or gene replacement

 

Advantages

  • GeneCopoeia is an expert in CRISPR/TALEN technologies and a full service provider for genome editing projects.

 

To Order

To request a quote for CRISPR- or TALEN-based stable cell line services, please fill out the inquiry form.

Request a Quote

 

Related Products