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Proteomic Antigen Array

GeneCopoeia’s OmicsArray™ Proteomic Antigen Array is a high-density protein microarray enabling powerful profiling of antibodies and other interacting proteins for many applications. The array carries nearly 16,000 proteins, expressed in E. coli, spotted onto a coated glass slide. It permits the profiling of known interacting proteins in samples. One of the most powerful applications for the OmicsArray™ Mammalian16K Protein Microarray is the detection of antibodies  in patient body fluids that are associated with autoimune diseases, as well as the potential discovery of novel autoantibodies.

GeneCopoeia’s OmicsArray™ Proteomic Antigen Array is available through our custom array profiling service, which includes sample processing and incubation with the array, data acquisition and normalization, and data analysis. This array is not available for purchase without the service.

In addition to this premade array, arrays containing customized sets of antigens are available, as well as array profiling services and data analysis. To order premade or custom arrays, please contact us.

GeneCopoeia’s OmicsArray™ Proteomic Antigen Array is part of the GeneCopoeia OmicsArray™ Antigen Microarray family.

Return to the main OmicsArray™ Antigen Microarray page

OmicsArray™ premade antigen arrays

For pricing, please inquire.

Cat. No. Description Application Antigen list
PA014 Proteomic Antigen Array – contains ~16,000 mammalian proteins Discovering novel autoantibody biomarkers in various immune-associated diseases Inquire



In addition to premade arrays, arrays containing customized sets of antigens are available, as well as array processing kits, array profiling services and data analysis.


Custom services

GeneCopoeia offers custom antigen microarray services in the following areas:

  • Custom array printing. GeneCopoeia will create custom antigen microarrays built to your specifications.
  • Sample processing. Send us your blood, plasma, tissue, or other biological sample and we will prepare it for processing and incubation with any of our premade antigen microarrays or custom-built antigen microarrays. For information on sample types to submit, consult the FAQ
  • Data analysis. Once samples are processed and incubated with an antigen microarray, we will analyze the raw data. The standard analysis service includes: 1) An Excel file of the Net Signal Intensity (NSI) for each antigen on the array, normalized to internal controls; and 2) a heat map

Additional analysis services, including proteomic analysis, pathway analysis, and more, are also available.

To inquire about custom antigen microarray services, please click here to contact us.


Technology overview

Technology overview

GeneCopoeia's OmicsArray™ antigen microarrays contain up to 120 purified proteins spotted onto nitrocellulose filters, which are adhered to glass slides. In addition, 8 spots are included for normalization. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control. As little as 1 ul serum or 50 ul of other bio fluids are needed for each sample.

As shown in Figure 1, arrays are incubated with patient samples, and any antibodies in the  samples bind to their cognate antigens on the array. The arrays are washed to remove unbound antibodies and other proteins, then co-incubated with Cy3- and Cy5-labeled secondary antibodies. The dual labeling strategy is intended to distinguish between immunoglobulin (Ig) subtypes present within samples. For example. a Cy3-labeled anti-IgG secondary antibody is used to detect IgG antibodies, and a Cy5-labeled anti-IgM secondary antibody is used to detect IgM antibodies. Fluorophore-labeled secondary antobodies are available for detecting IgA, IgD, IgE, IgG and IgM immunoglubulins, as well as IgG subclasses IgG1, IgG2, IgG3, and IgG4.

After washing to remove unbound secondary antibodies, signals are detected using a GenePix® 4400A microarray scanner. The raw data is then analyzed using GenePix® 7.0 software.


Figure 1. Workflow for detection of antibodies in samples using GeneCopoeia's OmicsArray™ antigen microarrays.

Return to the main OmicsArray™ Antigen Microarray page