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OmicsArray™ Antigen Microarray Products and Services

GeneCopoeia's OmicsArray™ antigen microarrays are high-throughput protein microarrays (also known as "protein arrays" or "protein chips") and are powerful tools for many applications, including autoantibody profiling, biomarker detection, pathogen-specific antibody identification, and more. Antigen microarrays enable parallel detection of antibodies or other biomarkers present in multiple patient samples for diseases including:

  • Systemic lupus erythematosus
  • Rheumatoid arthritis
  • Sjögren's Syndrome
  • Type 1 diabetes
  • Allergies
  • Pathogen infections such as COVID-19
  • Autoimmunity resulting from immune checkpoint inhibition therapy
  • Central nervous system disorders such as Alzheimer's disease

Each array contains superior-quality purified antigens printed onto nitrocellulose filters, which are adhered to glass slides. Antigens known to be associated with specific diseases are chosen based on a thorough review of peer-reviewed publications.

In addition to premade arrays, arrays containing customized sets of antigens are available, as well as array processing kits, array profiling services and data analysis.

Features
  • Multiplexing.  OmicsArray™ antigen microarrays can assay hundreds to thousands of antigens at a time, compared with 1 protein at a time for ELISA.
  • High throughput.  Each slide can process up to 15 samples in parallel.
  • High sensitivity. Each array can detect as little as 1 pg/ml of antibody, which is 100-fold more sensitive than ELISA.
  • Small sample volume. As little as 1 ul of serum is needed for detection.
  • Fast turnaround time. From sample-to-data in as little as 2 weeks.

 

Premade arrays and reagent kits

To order custom services, please refer to the "Custom services" section.

Use the drop-down menus below to expand the list of products for each category

OmicsArray™ premade antigen microarrays

Prices are for each slide, which carry 16 identical arrays.

Buy Catalog number Number of Antigens Description Price, Academic1,2 Price, Industry1,2
PA001 120 Human autoantigens general survey $1,500 $2,250
PA002 120 Human brain and central nervous system disorders $2,367 $3,550
PA003 120 Human cancer and neoplasms $1,933 $2,899
PA006 75 Human common allergens $1,174 $1,761
PA010 120 Human Autoimmunity, Allergy, and Infection $1,500 $2,250
PA012 120 Human Coronavirus-associated Autoimmunity (CAA) $1,500 $2,250
PA013 58 SARS-CoV-2 coronavirus variant proteins $993 $1,489
  PA014 15,626 NEW! 16K Human Protein Microarray (service only) Inquire Inquire

 

 

1Prices are for the slides only, and do not include processing and analysis services.

2Prices are for customers from the U.S. and Canada only. For other customers, please contact your local distributor for details.

To inquire about a custom antigen microarray, please contact inquiry@genecopoeia.com.

OmicsArray™ antigen microarray processing kits

GeneCopoeia's OmicsArray™ Antigen Microarray Processing Kits for human and mouse contain all the reagents needed for users with a compatible microarray scanner (not included) to incubate samples (serum, plasma, cerebrospinal fluid. and other body fluids) with OmicsArray™ Antigen Microarrays: Hybridization and washing buffers, DNase I, DTT, and anti-human or anti-mouse labeled secondary antibodies (Cy3-IgG, Cy5-IgM). Slide trays and gaskets sold separately.

Buy Catalog number Description Price
PA100 OmicsArray™ Antigen Microarray Processing Kit, Human (for processing up to 4 slides) $250
PA101 OmicsArray™ Antigen Microarray Processing Kit, Mouse (for processing up to 4 slides) $250
PA102 OmicsArray™ 4-slide Tray and Gasket Set $325
PA103 OmicsArray™ 16-well Slide Gasket (pack of 2) $100

 

 

Custom services

GeneCopoeia offers custom antigen microarray services in the following areas:

  • Custom array printing. GeneCopoeia will create custom antigen microarrays built to your specifications.
  • Sample processing. Send us your blood, plasma, tissue, or other biological sample and we will prepare it for processing and incubation with any of our premade antigen microarrays or custom-built antigen microarrays. For information on sample types to submit, consult the FAQ
  • Data analysis. Once samples are processed and incubated with an antigen microarray, we will analyze the raw data. The standard analysis service includes: 1) An Excel file of the Net Signal Intensity (NSI) for each antigen on the array, normalized to internal controls; and 2) a heat map

Additional analysis services, including proteomic analysis, pathway analysis, and more, are also available.

To inquire about custom antigen microarray services, please contact inquiry@genecopoeia.com.

Technology overview

Technology overview

GeneCopoeia's OmicsArray™ antigen microarrays contain up to 120 purified antigens spotted onto nitrocellulose filters, which are adhered to glass slides. In addition, 8 spots are included for normalization. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control. As little as 1 ul serum or 50 ul of other bio fluids are needed for each sample.

As shown in Figure 1, arrays are incubated with patient samples, and any antibodies in the  samples bind to their cognate antigens on the array. The arrays are washed to remove unbound antibodies and other proteins, then co-incubated with Cy3- and Cy5-labeled secondary antibodies. The dual labeling strategy is intended to distinguish between immunoglobulin (Ig) subtypes present within samples. For example. a Cy3-labeled anti-IgG secondary antibody is used to detect IgG antibodies, and a Cy5-labeled anti-IgM secondary antibody is used to detect IgM antibodies. Fluorophore-labeled secondary antobodies are available for detecting IgA, IgD, IgE, IgG and IgM immunoglubulins, as well as IgG subclasses IgG1, IgG2, IgG3, and IgG4.

After washing to remove unbound secondary antibodies, signals are detected using a GenePix® 4400A microarray scanner. The raw data is then analyzed using GenePix® 7.0 software.

 

Figure 1. Workflow for detection of antibodies in samples using GeneCopoeia's OmicsArray™ antigen microarrays.