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OmicsArray™ Antigen Microarray Products and Services

GeneCopoeia's OmicsArray™ antigen microarrays are high-throughput protein microarrays (also known as "protein arrays" or "protein chips") that are powerful tools for many applications, including autoantibody profiling, biomarker detection, pathogen-specific antibody identification, and more. Antigen microarrays enable detection of hundreds to thousands of antibodies or other biomarkers present in multiple samples in parallel.

Each array contains superior-quality purified antigens printed onto nitrocellulose filters, which are adhered to glass slides. Antigens known to be associated with specific diseases or pathogens are chosen based on a thorough review of peer-reviewed publications.

 

Advantages of OmicsArray™ Antigen Microarrays
  • Largest collection of pre-made whole-protein antigen microarrays on the market.
  • Largest number of whole-protein antigens specifically focused on autoimmune diseae research.
  • Best combination of number of antigens per array (up to 120) with number of samples that can be processed per slide (up to 15).

 

OmicsArray™ premade antigen microarrays

For pricing, please inquire.

Cat. No. Description Application Antigen list
PA001 Systemic Autoimmune-associated Antigen Array Profiling autoantibodies for lupus, Sjögren’s and other systemic rheumatic diseases Download
PA002 Brain and Neuronal-associated Antigen Array Profiling autoantibodies associated with brain and neuronal disorders Download
PA003 Cancer and Neoplasm-associated Antigen Array Profiling autoantibodies associated with cancer and cancer checkpoint immunotherapy Download
PA006 Allergen Antigen Array Profiling antibodies (IgG, IgE) reacting with common allergens Download
PA010 Infection and Vaccination-associated Autoimmune Antigen Array Profiling antibodies associated with common virus and vaccine induced autoimmunity Download
PA012 Coronavirus infection-associated Autoimmune Antigen Array Profiling antibodies associated with coronavirus infection and vaccination induced autoimmunity Download
PA013 SARS-CoV-2 and Variants Antigen Array Profiling antibody reactivities with the spectrum of antigens of SARS-CoV-2 and variants Download
PA015 NEW! Ganglioside and Phospholipid Antigen Array Profiling autoantibodies against gangliosides and phospholipids in autoimmune neuropathies Download
PA014 NEW! Proteomic Antigen Array – contains ~16,000 mammalian proteins Discovering novel autoantibody biomarkers in various immune-associated diseases Inquire

 

 

 

In addition to premade arrays, arrays containing customized sets of antigens are available, as well as array processing kits, array profiling services and data analysis.

 

Antigen microarray processing kits

GeneCopoeia's OmicsArray™ Antigen Microarray Processing Kits for human and mouse contain all the reagents needed for users with a compatible microarray scanner (not included) to incubate samples (serum, plasma, cerebrospinal fluid. and other body fluids) with OmicsArray™ Antigen Microarrays: Hybridization and washing buffers, DNase I, DTT, and anti-human or anti-mouse labeled secondary antibodies (Cy3-IgG, Cy5-IgM). Slide trays and gaskets sold separately.

Prices are for customers from the U.S. and Canada only. For other customers, please contact your local distributor for details.

Buy Catalog number Description Price
PA100 OmicsArray™ Antigen Microarray Processing Kit, Human (for processing up to 4 slides) $250
PA101 OmicsArray™ Antigen Microarray Processing Kit, Mouse (for processing up to 4 slides) $250
PA102 OmicsArray™ 4-slide Tray and Gasket Set $325
PA103 OmicsArray™ 16-well Slide Gasket (pack of 2) $100

 

User Manual:

 

SDS:

 

Custom services

GeneCopoeia offers custom antigen microarray services in the following areas:

  • Custom array printing. GeneCopoeia will create custom antigen microarrays built to your specifications.
  • Sample processing. Send us your blood, plasma, tissue, or other biological sample and we will prepare it for processing and incubation with any of our premade antigen microarrays or custom-built antigen microarrays for autoantibody profiling and other applications. For information on sample types to submit, consult the FAQ
  • Data analysis. Once samples are processed and incubated with an antigen microarray, we will analyze the raw data. The standard analysis service includes: 1) An Excel file of the Net Signal Intensity (NSI) for each antigen on the array, normalized to internal controls; and 2) a heat map

Additional analysis services, including proteomic analysis, pathway analysis, and more, are also available.

To inquire about custom antigen microarray products and services, please fill out our custom quote request form

 

Technology overview

Technology overview

GeneCopoeia's OmicsArray™ antigen microarrays contain up to 120 purified antigens spotted onto nitrocellulose filters, which are adhered to glass slides. In addition, 8 spots are included for normalization. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control. As little as 1 ul serum or 50 ul of other bio fluids are needed for each sample.

As shown in Figure 1, arrays are incubated with patient samples, and any autoantibodies in the  samples bind to their cognate antigens on the array. The arrays are washed to remove unbound autoantibodies and other proteins, then co-incubated with Cy3- and Cy5-labeled secondary antibodies. The dual labeling strategy is intended to distinguish between immunoglobulin (Ig) subtypes present within samples. For example. a Cy3-labeled anti-IgG secondary antibody is used to detect IgG autoantibodies, and a Cy5-labeled anti-IgM secondary antibody is used to detect IgM autoantibodies. Fluorophore-labeled secondary antobodies are available for detecting IgA, IgD, IgE, IgG and IgM immunoglubulins, as well as IgG subclasses IgG1, IgG2, IgG3, and IgG4.

After washing to remove unbound secondary antibodies, signals are detected using a GenePix® 4400A microarray scanner. The raw data is then analyzed using GenePix® 7.0 software.

 

Figure 1. Workflow for autoantibody profiling in samples using GeneCopoeia's OmicsArray™ antigen microarrays.