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    OmicsLink™ shRNA Clone Collections

    Introduction OmicsLink shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect.

    Guaranteed shRNA knockdown

    A set of three expression constructs and a scrambled control is offered against every target gene with the guarantee that at least one of the three will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge. For more about our guaranteed shRNA knockdown, refer to warranty and cancellation policy here.

    All cell types covered

    Lentiviral and non-viral vector options allow the transfection or transduction of shRNA into difficult-to-transfect cells as well as more conventional cell lines.

    Promoters of your choice

    shRNA is driven by pol III promoters (H1, U6) or pol II promoters (CMV, EF1a, CAG and CBh), both with great knockdown efficiency. Request a quote for pol II promoter-driven shRNA lentivirus or AAV services. pol III promoter-driven shRNA pol II promoter-driven shRNA

    Multiple delivery formats

    shRNAs are delivered as 3 individual constructs of 5 µg purified plasmid and a separate scrambled control plasmid. Lentivirus and AAV for shRNA are also available.

    Markers and reporters

    Vectors with mCherry or eGFP reporter genes for monitoring transfection or transduction efficiencies. Stable cell selection with puromycin marker.

    Fully sequenced expression cassettes

    Expression cassettes of all shRNA clones are fully sequenced including the promoter, sense and antisense target sequences, hairpin, terminator, and other linker sequences.

    Figure 1. Lentiviral and non-viral expression vector-based shRNA clones