CRISPRa/i Stable Cell Lines
GeneHero™ CRISPRa Stable Cell Lines are mammalian cell lines stably expressing synergistic activation mediators (SAM) for the study of transcriptional activation of endogenous genes. These CRISPRa cell lines have stably integrated human codon-optimized dCas9 fused at the C-terminus of transcriptional activator VP64, and activation helper protein complex MPH (MS2-p65-HSF1).
GeneHero™ CRISPRi Stable Cell Lines are mammalian cell lines stably expressing dCas9-KRAB fusion protein for the study of transcriptional inhibition of endogenous genes. These CRISPRi cell lines have stably integrated human codon-optimized dCas9 fused to a transcriptional repressor peptide KRAB to silence target gene expression.
