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qPCR Mix & Primers

 

Quantitative PCR (qPCR) is one of the most widely-used methods for gene expression profiling, due to its sensitivity, speed, and relatively low cost.  For the two-step real-time RT-qPCR system, GeneCopoeia provides three kinds of qPCR mix, experimentally-validated primers, and a first-strand cDNA synthesis kit for you to detect and quantitate your gene expression.

 

BlazeTaq™ SYBR Green qPCR Mix 2.0

It contains a hot-start antibody-modified Taq DNA polymerase and an optimized buffer system that avoids non-specific amplification of target DNA at lower temperatures and enhances reaction mix performance. 

BlazeTaq™ Probe qPCR Mix

It uses hydrolysis probes and DNA as starting material. The kit is supplied with a ready-to-use 5X master mix with a hot-start Taq DNA polymerase, dNTP and all optimized buffer components.

All-in-One™ qPCR Mix and Validated Primers

It uses high-fidelity hot-start polymerase, an optimized reaction buffer and high-quality dNTPs to enable specific and sensitive amplification from even low-copy RNA (cDNA) or DNA species.

SureScript™ First-Strand cDNA Synthesis Kits

The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.

 

 

      SYBR green-based    

       Probe-based           

Specificity Medium High
Reproducibility Medium High
Multiplexing No Yes

Application

Gene expression

DNA quantitation

CHiP

Gene expression

DNA quantitation

CHiP

SNP genotyping

Copy number variation

Pathway analysis

Mutation detection