Quantitative PCR (qPCR) is one of the most widely-used methods for gene expression profiling, due to its sensitivity, speed, and relatively low cost. For the two-step real-time RT-qPCR system, GeneCopoeia provides three kinds of qPCR mix, experimentally-validated primers, and a first-strand cDNA synthesis kit for you to detect and quantitate your gene expression.
BlazeTaq™ SYBR Green qPCR Mix 2.0
It contains a hot-start antibody-modified Taq DNA polymerase and an optimized buffer system that avoids non-specific amplification of target DNA at lower temperatures and enhances reaction mix performance.
BlazeTaq™ Probe qPCR Mix
It uses hydrolysis probes and DNA as starting material. The kit is supplied with a ready-to-use 5X master mix with a hot-start Taq DNA polymerase, dNTP and all optimized buffer components.
All-in-One™ qPCR Mix and Validated Primers
It uses high-fidelity hot-start polymerase, an optimized reaction buffer and high-quality dNTPs to enable specific and sensitive amplification from even low-copy RNA (cDNA) or DNA species.
SureScript™ First-Strand cDNA Synthesis Kits
The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.
|
SYBR green-based |
Probe-based | |
| Specificity | Medium | High |
| Reproducibility | Medium | High |
| Multiplexing | No | Yes |
|
Application |
Gene expression DNA quantitation CHiP |
Gene expression DNA quantitation CHiP SNP genotyping Copy number variation Pathway analysis Mutation detection |
* Promotion is valid in the US & Canada only. For international customers, please contact your local distributors. Promotion ends July 31, 2023. Discounts are not valid on previous purchases and cannot be combined with additional discounts.