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One-step RT-qPCR Kits

30% off SYBR Green & Probe One-Step Kits!*

A one-step RT-qPCR kit uses RNA as the starting material. It provides a convenient master mix to convert mRNA to cDNA and then quantify the cDNA in a one-step reaction. GeneCopoeia offers SYBR green- and probe-based one-step RT-qPCR kits that enable accurate analysis of gene expression in a convenient and high-throughput one-step format.

BlazeTaq™ SYBR Green One-Step RT-qPCR Kit

BlazeTaq™ One-Step SYBR Green RT-qPCR kit uses a reverse transcriptase to convert RNA to cDNA, and an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components.

 

BlazeTaq™ Probe One-Step RT-qPCR Kit

BlazeTaq™ Probe One-Step RT-qPCR kit allows potent target RNA detection and quantification using hydrolysis probes in a single tube. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components.

 

 

      SYBR green-based    

       Probe-based           

Specificity Medium High
Reproducibility Medium High
Multiplexing NO Yes

Application

Gene expression

DNA quantitation

CHiP

Gene expression

DNA quantitation

CHiP

SNP genotyping

Copy number variation

Pathway analysis

Mutation detection

 

* Promotion is valid in the US & Canada only. Discount is valid on BlazeTaq SYBR Green- based & Probe- based qPCR mixes & kits. Discount is only valid for 600-1000 rxns. No additional discount will be provided. Not valid on previous purchases. Offer ends March 31, 2020.