A one-step RT-qPCR kit uses RNA as the starting material. It provides a convenient master mix to convert mRNA to cDNA and then quantify the cDNA in a one-step reaction. GeneCopoeia offers SYBR green- and probe-based one-step RT-qPCR kits that enable accurate analysis of gene expression in a convenient and high-throughput one-step format.
BlazeTaq™ SYBR Green One-Step RT-qPCR Kit
BlazeTaq™ One-Step SYBR Green RT-qPCR kit uses a reverse transcriptase to convert RNA to cDNA, and an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components.
BlazeTaq™ Probe One-Step RT-qPCR Kit
BlazeTaq™ Probe One-Step RT-qPCR kit allows potent target RNA detection and quantification using hydrolysis probes in a single tube. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components.
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SYBR green-based |
Probe-based |
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|---|---|---|
Specificity |
Medium | High |
Reproducibility |
Medium | High |
Multiplexing |
NO | Yes |
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Application |
Gene expression DNA quantitation CHiP |
Gene expression DNA quantitation CHiP SNP genotyping Copy number variation Pathway analysis Mutation detection |