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Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentivirus

GeneCopoeia's Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products produce a specially-packaged version of standard lentivirus, whereby the vesicular stomatitis G (VSV-G) envelope glycoprotein is replaced by the Spike (S) protein from SARS-CoV-2, the virus responsible for the COVID-19 pandemic. These particles, also known as "pseudoviruses", are typically used to infect cultured cells expressing ACE2, the receptor for SARS-CoV-2 on multiple  tissue types (Figure 1)..

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus and our ACE2- and TMPRSS2-expressing cell lines are great for several applications in a safe environment without the use of pathogenic SARS-CoV-2 viruses, such as:

  • Assessment of the efficacy of vaccines against SARS-CoV-2 virus variants
  • Studying the efficacy and mechanism of neutralizing antibodies against SARS-CoV-2 virus variants
  • Development of antiviral therapeutic agents
  • Studying the mechanism of virus-receptor interaction

 

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products are part of GeneCopoeia's COVID-19 Coronavirus Research Tools products. Note: Spike-pseudotyped lentiviruses are for research purposes only. They are not intended for diagnostic or treatment usage in humans.

Ordering information

Ordering information

Use the drop-down menus below to view products for lentivirus packaged with SARS-CoV-2 Spike protein.

For more information, please contact inquiry@genecopoeia.com.

SARS-CoV-2 Spike protein-pseudotyped lentiviral particles

GeneCopoeia's Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentivirus is available as either premade, in vitro grade or custom-produced in vivo grade particles.

Advantages
  • Accurate functional titers.  Functional titers are measured by FACS sorting of ACE2-expressing HEK293T cells infected with GFP-expressing particles.
  • Complete system. Premade, in vitro-grade purified particles can be used with GeneCopoeia's ACE2-expressing HEK293T cells as well as other cells permissive to SARS-CoV-2 infection. Kits for do-it-yourself packaging of Spike-pseudotyped lentivirus also available.
  • Quantitative measurements. Expression of GFP and luciferase enables quantitative determination of infection efficiency that is more accurate than traditional plaque reduction assays.
  • Safe. Study SARS-CoV-2 Spike-mediated interactions with cells in the absence of pathogenic virus.

 

    For more information, please contact inquiry@genecopoeia.com.

    Lentifect™ lentiviral particles for SARS-CoV-2 coronavirus research (in vitro grade)

    NOTE: Use the filters to select/de-select options for the type of envelope protein and which SARS-CoV-2 Spike variant you want expressed on the surface of your SARS-CoV-2 Spike pseudotyped lentivirus.

    Envelope protein :   Spike variant
    Buy Catalog Product Description Spike variant4 Envelope protein Reporter / marker Vector Price
    SP006-100

    Coming soon!

    HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (UK B.1.1.7 & D614G) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl UK B.1.1.7 & D614G Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP007-100

    Coming soon!

    HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (South African B.1.351 & D614G) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl South African B.1.351 & D614G Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP008-100

    Coming soon!

    HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (Brazil B.1.1.28.1) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl Brazil B.1.1.28.1 Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP101-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length Spike-Pseudotyped Lentiviral Particles (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl WT Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP103-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length D614G Spike-Pseudotyped Lentiviral Particles (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl D614G Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP104-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length N439K Spike-Pseudotyped Lentiviral (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl N439K Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP105-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (D614G & N439K) Spike-Pseudotyped Lentiviral (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl D614G & N439K Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP106-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (UK B.1.1.7 & D614G) Spike-Pseudotyped Lentiviral (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl UK B.1.1.7 & D614G Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP107-100

    Coming soon!

    HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (South African B.1.351 & D614G) Spike-Pseudotyped Lentiviral (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl South African B.1.351 & D614G Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP108-100

    Coming soon!

    HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 full-length (Brazil B.1.1.28.1) Spike-Pseudotyped Lentiviral (50 µl x 2 vials) > 1×105 TU/ml1 or > 1×107 RFU/ml* lentiviral particles ready for transduction, 100 µl Brazil B.1.1.28.1 Full-length Spike eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP001-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×106 TU/ml1 or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µll WT Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP003-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 D614G Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×106 TU/ml1 or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µll D614G Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP004-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 N439K Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×106 TU/ml1 or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µll N439K Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP005-100 HLUC-Lv201 Firefly Luciferase + eGFP Lentifect™ SARS-CoV-2 (D614G & N439K) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (50 µl x 2 vials) > 1×106 TU/ml1 or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µll D614G & N439K Truncated Spike* eGFP/Puro, HLUC pReceiver-Lv201 $825
    LP464-025 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl N/A standard VSV-G glycoprotein eGFP/Puro, HLUC pReceiver-Lv201 $295
    LP464-100 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 4 vials) > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl N/A standard VSV-G glycoprotein eGFP/Puro, HLUC pReceiver-Lv201 $725
    SP401-025 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 1 vial) > 1×1010 copies/ml3 lentiviral particles ready for transduction, 100 µl N/A None eGFP/Puro, HLUC pReceiver-Lv201 $295
    SP401-100 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 4 vial) > 1×1010 copies/ml3 lentiviral particles ready for transduction, 100 µl N/A None eGFP/Puro, HLUC pReceiver-Lv201 $725

     

     

    1 The functional titers of SARS-CoV-2 Spike pseudotyped lentivirus are determined by FACS sorting of GFP positive ACE2-expressing ACE2/HEK293T cells (GeneCopoeia Cat.No. SL221) and by luciferase analysis (GeneCopoeia Cat.No. LF007).

    2Titers of standard VSV-G pseudotyped lentivirus are determined by RT-qPCR to determine physical titer (copies/ml) and extrapolated to functional titer (TU/ml).

    3The titer of the lentiviral particles without envelope protein is determined by RT-qPCR. 

    4SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

    *Truncated SARS-CoV-2 Spike proteins carry a deletion of the 19 amino acid C-terminal endoplasmic reticulum retention signal.

    COVID-19 Coronavirus Receptor Stable Cell Lines

    Need a cell line to infect with your SARS-CoV-2 Spike pseudotyped lentiviral particles? GeneCopoeia has produced HEK293T cell lines expressing human ACE2 alone, or ACE2 in combination with TMPRSS2, a protease that promotes Spike-ACE2 receptor interaction. These cells are amenable to infection by viruses expressing SARS-CoV-2 Spike protein in their envelopes. For more detailed information regarding these cell lines, please visit our COVID-19 Coronavirus Receptor Stable Cell Lines page.

    You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

    In addition to the products below, custom stable cell lines are also available. For more information, please contact inquiry@genecopoeia.com.

    Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
    SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $1395 $3995
    SL222 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells Embryonic kidney

    mCherry/

    Puromycin, 

    Hygromycin

    $1795 $4795

     

      Warranty statement and limited use license   MSDS
     

     

    For more information or to request a quote, please contact inquiry@genecopoeia.com.

    Lentivirus packaging kits for SARS-CoV-2 coronavirus research

    Lenti-Pac™ lentivirus packaging kits for SARS-CoV-2 coronavirus research

      GeneCopoeia manufactures kits that allow researchers to produce SARS-CoV-2 Spike-pseudotyped lentivirus, which you can order from the table below.

      Buy Catalog# Product Description Spike variant1 Price
      Kits for packaging lentivirus pseudotyped with full-length Spike protein

      LT011

      Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) $745
      LT015 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G $745

      LT041

      Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (UK B.1.1.7 & D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent UK B.1.1.7 & D614G $745

      LT044

      Coming soon!

      Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (South African B.1.351 & D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent South African B.1.351 & D614G $745

      LT047

      Coming soon!

      Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (Brazil B.1.1.28.1) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent Brazil B.1.1.28.1 & D614G $745
      Kits for packaging lentivirus pseudotyped with truncated Spike protein (ER retention signal removed)

      LT021

      Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) $745
      LT017 Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G $745
      Kits for packaging lentivirus with standard VSV-G glycoprotein
      LT001 Lenti-Pac™ HIV Expression Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent N/A $592
      LT002 Lenti-Pac™ HIV Expression Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent N/A $989
      Control lentiviral clones that can be packaged into lentiviral particles
      EX-hLUC-Lv201 Control vector (with hLUC and eGFP) for pReceiver-Lv201 Control vector (with hLUC and eGFP) for pReceiver-Lv201 N/A $349
      Additional reagents recommended for lentiviral packaging
      LT005 Lenti-Pac™ HIV qRT-PCR Titration Kit (20 RT reactions, 50 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. N/A $285
      LT006 Lenti-Pac™ HIV qRT-PCR Titration Kit (40 RT reactions, 100 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. N/A $430
      LT007 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles N/A $139
      LT008 Lenti-Pac™ 293Ta Cell Line (1.5 x 106 cells)-for packinging lentivirus with VSV-G only 293Ta Lentiviral packaging cell line-for packinging lentivirus with VSV-G only N/A $250
      SL221 HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells N/A $1395 academic, $3995 industry
      SL222 HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2(NM_005656.3), 2×106 cells N/A $1795 academic, $4795 industry
      MP004 Mycoplasma PCR detection kit (50 rxns) Quick detection of mycoplasma contamination in cell culture or virus N/A $125

       

        User Manual
       

       LT011

       LT015 

       LT041 

       LT044   LT047 

       LT017 

       LT021 

      (LT001/LT002)

       

       

       

      1SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

      For more information or to request a quote, please contact inquiry@genecopoeia.com.

      HEK293T receptor cell lines expressing ACE2 and TMPRSS2

      GeneCopoeia has produced HEK293T cell lines expressing human ACE2 alone, or ACE2 in combination with TMPRSS2, a protease that promotes Spike-ACE2 receptor interaction. These cell lines express ACE2 and/or TMPRRSS2 mRNA at high levels (Figure 1). Due to display of ACE2 protein (Figure 2) and/or TMPRSS2 protein ((Figure 3) on the cell surface, these cells are amenable to infection by viruses expressing SARS-CoV-2 Spike protein in their envelopes.

      You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

      In addition to the products below, custom stable cell lines are also available. For more information, please contact inquiry@genecopoeia.com.

      Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
      SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $1395 $3995
      SL222 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells Embryonic kidney

      mCherry/

      Puromycin, 

      Hygromycin

      $1795 $4795

       

        Warranty statement and limited use license   MSDS
       

       

      For more information or to request a quote, please contact inquiry@genecopoeia.com.

      Performance data

      Figure 1. Relative mRNA expression levels of ACE2 and TMPRSS2 in GeneCopoeia stable cell lines. mRNA was amplified via RT-qPCR using primers specific for each gene. Ct values of ACE2 and TMPRSS2 were normalized to Ct values for housekeeping gene GAPDH. Relative expression levels were calculated from 2^ΔΔCt. A. ACE2 mRNA expression in parental cell line HEK293T and ACE2-overexpressing HEK293T cell line SL221. B. ACE2 and TMPRSS2 mRNA expression in parental cell line HEK293T and ACE2- and TMPRSS2-overexpressing cell line SL222.

      Figure 2. Immunofluorescence (IF) analysis of ACE2 protein expression on the cell surface of ACE2-expressing HEK293T cells (Cat.# SL221), ACE2/TMPRSS2 expressing HEK293T cells (Cat.# SL222), and HEK293T cells. Unpermeablized cells were fixed, blocked and incubated with the primary anti-ACE2 antibody, then washed and incubated with  fluorescent-labeled secondary antibody, washed again, and stained with DAPI.

       

      Figure 3. Immunofluorescence (IF) analysis of TMPRSS2 protein expression on the cell surface of TMPRSS2-expressing HEK293T cells (Cat.# SL222), and HEK293T cells. Unpermeablized cells were fixed, blocked and incubated with the primary anti-TMPRSS2 antibody, then washed and incubated with  fluorescent-labeled secondary antibody, washed again, and stained with DAPI.

       

      Firefly luciferase assay kits

      Luc-Pair™ Firefly Luciferase HS Assay Kit  

      Buy Catalog# Product Description Price
      LF007 Luc-Pair™ Firefly Luciferase HS Assay Kit (100rxns) High sensitivity for detecting the activity of Firefly luciferase. $77
      LF008 Luc-Pair™ Firefly Luciferase HS Assay Kit (300rxns) $125
      LF009 Luc-Pair™ Firefly Luciferase HS Assay Kit (1000rxns) $375
      LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $40

        User manual MSDS