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Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentivirus

GeneCopoeia's Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products produce a specially-packaged variant of standard lentivirus, whereby the vesicular stomatitis G (VSV-G) envelope glycoprotein is replaced by the Spike (S) protein from SARS-CoV-2, the virus responsible for the COVID-19 pandemic (Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052). These particles can be used in vitro, to infect cultured cells expressing ACE2, the receptor for SARS-CoV-2 on multiple  tissue types (Figure 1), or in vivo in animal models.

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus and our ACE2- and TMPRSS2-expressing cell lines are great for several applications, such as:

  • Vaccine development for prevention of infection by SARS-CoV-2 virus
  • Studying the efficacy and mechanism of neutralizing antibodies against SARS-CoV-2 virus
  • Development of antiviral therapeutic agents
  • Studying the mechanism of virus-receptor interaction

 

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products are part of GeneCopoeia's COVID-19 Coronavirus Research Tools products. Note: Spike-pseudotyped lentivirus is for research purposes only. It is not intended for diagnostic or treatment usage in humans.

Ordering information

Ordering information

Use the drop-down menus below to view products for lentivirus packaged with SARS-CoV-2 Spike protein.

For more information, please contact inquiry@genecopoeia.com.

SARS-CoV-2 Spike protein-pseudotyped lentivirus particle kits

GeneCopoeia's Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentivirus is available as either premade, in vitro grade or custom-produced in vivo grade particles.

Advantages
  • Accurate functional titers.  Functional titers are measured by FACS sorting of ACE2-expressing HEK293T cells infected with GFP-expressing particles.
  • Complete system. Premade, in vitro-grade purified particles can be used with GeneCopoeia's ACE2-expressing HEK293T cells as well as other ACE2-expressing cells. Kits for do-it-yourself packaging of Spike-pseudotyped lentivirus also available.
  • Application versatility.
    • Spike-pseudotyped lentivirus can be packaged for either in vitro grade or ultra-purified in vivo grade.
    • Viral particles can express either commonly-used markers such as GFP and luciferase, or most human, mouse, and rat ORFs in GeneCopoeia's >80 lentiviral transfer vector types.

 

    For more information, please contact inquiry@genecopoeia.com.

    Lentifect™ lentiviral particles for SARS-CoV-2 coronavirus research (in vitro grade)

    Buy Catalog# Product Description Spike variant4 Envelope tag Reporter / marker Vector Price
    Lentifect™ lentivirus pseudotyped with full-length Spike protein
    SP101-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length Spike-Pseudotyped Lentiviral Particles (25 µl x 4 vial) > 1×105 TU/ml1 or > 1×107 RFU/ml* control lentiviral particles ready for transduction, 100 µl WT (D614) None eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP103-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length D614G Spike-Pseudotyped Lentiviral Particles (25 µl x 4 vial) > 1×105 TU/ml1 or > 1×107 RFU/ml* control lentiviral particles ready for transduction, 100 µl D614G None eGFP/Puro, HLUC pReceiver-Lv201 $825
    Lentifect™ lentivirus pseudotyped with truncated Spike protein (endoplasmic reticulum retention signal removed)
    SP001-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (25 µl x 4 vial) > 1×106 TU/ml1 or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µl WT (D614) None eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP003-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 D614G Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (25 µl x 4 vial) > 1×106 TU/ml1 or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µl D614G None eGFP/Puro, HLUC pReceiver-Lv201 $825
    SP201-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, C-flag tagged and with ER retention signal removed (25 µl x 4 vial) > 1×106 TU/ml1 or > 1×108 RFU/ml lentiviral particles ready for transduction, 100 µl WT (D614) C-Flag eGFP/Puro, HLUC pReceiver-Lv201 $825
    Lentifect™ lentiviral particles packaged with standard VSV-G glycoprotein
    LP464-025 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) > 1×108 TU/ml2 control lentiviral particles ready for transduction, 100 µl N/A None eGFP/Puro, HLUC pReceiver-Lv201 $295
    LP464-100 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 4 vial) > 1×108 TU/ml2 control lentiviral particles ready for transduction, 100 µl N/A None eGFP/Puro, HLUC pReceiver-Lv201 $725
    Lentifect™ lentivirus without envelope protein (negative control)
    SP401-025 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 1 vial) > 1×1010 copies/ml3 lentiviral particles ready for transduction, 100 µl N/A N/A eGFP/Puro, HLUC pReceiver-Lv201 $295
    SP401-100 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 4 vial) > 1×1010 copies/ml3 lentiviral particles ready for transduction, 100 µl N/A N/A eGFP/Puro, HLUC pReceiver-Lv201 $725

    1 The functional titers of SARS-CoV-2 Spike pseudotyped lentivirus are determined by FACS sorting of GFP positive ACE2-expressing ACE2/HEK293T cells (GeneCopoeia Cat.No. SL221) and by luciferase analysis (GeneCopoeia Cat.No. LF007, LF008, LF009) of transduced ACE2/HEK293T.

    2Titers of standard VSV-G pseudotyped lentivirus are determined by RT-qPCR to determine physical titer (copies/ml) and extrapolated to functional titer (TU/ml).

    3The titer of the lentiviral particles without envelope protein is determined by RT-qPCR. 

    4SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

         

     

    Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentivirus particle kits (in vitro grade)

    Kit includes:

    • Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentiviral particles
    • Lentifect™ standard VSV-G-pseudotyped lentiviral particles
    • Lentifect™ lentiviral particles packaged without envelope protein (negative control)
    • GeneCopoeia's ACE2-expressing HEK293T cell line

     

    Buy Catalog # Product Description Spike variant1 Envelope tag Reporter / marker Vector Price, Academic Price, Industry
    LT014 Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particle Kit SARS-CoV-2 spike-pseudotyped lentivirus (100 µl), lentivirus without envelope protein negative control  (25 µl x 1 vial), standard VSV-G lentivirus (25 µl x 1 vial), ACE2-expressing HEK293T cells (2 x 106 cells x 1 vial) WT (D614) None eGFP/Puro, HLUC pReceiver-Lv201 $2820 $4820
    LT024 Lentifect™ SARS-CoV-2 D614G Spike-Pseudotyped Lentiviral Particle Kit SARS-CoV-2 spike-pseudotyped lentivirus (100 µl), lentivirus without envelope protein negative control  (25 µl x 1 vial), standard VSV-G lentivirus (25 µl x 1 vial), ACE2-expressing HEK293T cells (2 x 106 cells x 1 vial) D614G None eGFP/Puro, HLUC pReceiver-Lv201 $2820 $4820
    LT013 Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particle Kit, ER retention signal removed SARS-CoV-2 spike-pseudotyped lentivirus (100 µl), lentivirus without envelope protein negative control  (25 µl x 1 vial), standard VSV-G lentivirus (25 µl x 1 vial), ACE2-expressing HEK293T cells (2 x 106 cells x 1 vial) WT (D614) None eGFP/Puro, HLUC pReceiver-Lv201 $2820 $4820
    LT023 Lentifect™ SARS-CoV-2 D614G Spike-Pseudotyped Lentiviral Particle Kit ER retention signal removed SARS-CoV-2 spike-pseudotyped lentivirus (100 µl), lentivirus without envelope protein negative control  (25 µl x 1 vial), standard VSV-G lentivirus (25 µl x 1 vial), ACE2-expressing HEK293T cells (2 x 106 cells x 1 vial) D614G None eGFP/Puro, HLUC pReceiver-Lv201 $2820 $4820

    1SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

         

     

    For more information or to request a quote, please contact inquiry@genecopoeia.com.

    Lentivirus packaging kits for SARS-CoV-2 coronavirus research

    Lenti-Pac™ lentivirus packaging kits for SARS-CoV-2 coronavirus research

      GeneCopoeia manufactures kits that allow researchers to produce SARS-CoV-2 Spike-pseudotyped lentivirus, which you can order from the table below.

       

      Buy Catalog# Product Description Spike variant1 Envelope tag Price
      Kits for packaging lentivirus pseudotyped with full-length Spike protein

      LT011

      Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) None $745

      LT012

      Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) None $1235
      LT015 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G None $745
      LT016 Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G None $1235
      Kits for packaging lentivirus pseudotyped with truncated Spike protein (ER retention signal removed)

      LT021

      Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) None $745

      LT022

      Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit, ER retention signal removed (40 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent WT (D614) None $1235
      LT017 Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G None $745
      LT018 Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit, ER retention signal removed (40 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent D614G None $1235
      Kits for packaging lentivirus with standard VSV-G glycoprotein
      LT001 Lenti-Pac™ HIV Expression Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent N/A None $592
      LT002 Lenti-Pac™ HIV Expression Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent N/A None $989
      Control lentiviral clones that can be packaged into lentiviral particles
      EX-hLUC-Lv201 Control vector (with hLUC and EGFP) for pReceiver-Lv201 Control vector (with hLUC and EGFP) for pReceiver-Lv201 N/A None $349
      Additional reagents recommended for lentiviral packaging
      LT005 Lenti-Pac™ HIV qRT-PCR Titration Kit (20 RT reactions, 50 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. N/A N/A $285
      LT006 Lenti-Pac™ HIV qRT-PCR Titration Kit (40 RT reactions, 100 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. N/A N/A $430
      LT007 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles N/A N/A $139
      LT008 Lenti-Pac™ 293Ta Cell Line (1.5 x 106 cells)-for packinging lentivirus with VSV-G only 293Ta Lentiviral packaging cell line-for packinging lentivirus with VSV-G only N/A N/A $250
      SL221 HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells N/A N/A $1395 academic, $3995 industry
      SL222 HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2(NM_005656.3), 2×106 cells HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2(NM_005656.3), 2×106 cells N/A N/A $1795 academic, $4795 industry
      MP004 Mycoplasma PCR detection kit (50 rxns) Quick detection of mycoplasma contamination in cell culture or virus N/A N/A $125

       

       

      User Manual

      (LT011/LT012)

      User Manual

      (LT021/LT022)

      User Manual

      (LT015/LT016)

      User Manual

      (LT017/LT018)

      User Manual

      (LT001/LT002)

       

      1SARS-CoV-2 strains carrying Spike amino acid mutant D614G have become, as of July, 2020, the prediminant global virus strains.

      For more information or to request a quote, please contact inquiry@genecopoeia.com.

      HEK293T cell lines expressing ACE2 and TMPRSS2

      GeneCopoeia carries HEK293T cell lines expressing human ACE2 alone, or ACE2 in combination with TMPRSS2, a protease that promotes Spike-ACE2 receptor interaction.

      You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

      In addition to the products below, custom stable cell lines, are also available. For more information, please contact inquiry@genecopoeia.com.

      Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
      SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $1395 $3995
      SL222 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2(NM_005656.3), 2×106 cells Embryonic kidney

      mCherry/

      Puromycin, 

      Hygromycin

      $1795 $4795

       

        Warranty statement and limited use license   MSDS
       

       

      For more information or to request a quote, please contact inquiry@genecopoeia.com.

      Firefly luciferase assay kits

      Luc-Pair™ Firefly Luciferase HS Assay Kit  

      Buy Catalog# Product Description Price
      LF007 Luc-Pair™ Firefly Luciferase HS Assay Kit (100rxns) High sensitivity for detecting the activity of Firefly luciferase. $77
      LF008 Luc-Pair™ Firefly Luciferase HS Assay Kit (300rxns) $125
      LF009 Luc-Pair™ Firefly Luciferase HS Assay Kit (1000rxns) $375
      LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $40

        User manual MSDS