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Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentivirus

GeneCopoeia's Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products produce a specially-packaged variant of standard lentivirus, whereby the commonly used vesicular stomatitis G (VSV-G) envelope glycoprotein is replaced in a proprietary plasmid mix expressing the Spike (S) protein from SARS-CoV-2, the virus responsible for the COVID-19 pandemic (Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052). These particles can be used in vitro, to infect cultured cells expressing ACE2, the receptor for SARS-CoV-2 on multiple  tissue types, or in vivo in animal models.

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus is great for several applications, such as:

  • Vaccine development for prevention of infection by SARS-CoV-2 virus
  • Studying the efficacy and mechanism of neutralizing antibodies against SARS-CoV-2 virus
  • Development of antiviral therapeutic agents
  • Studying the mechanism of virus-receptor interaction

 

Lentifect™ SARS-CoV-2 Spike-pseudotyped lentivirus products are part of GeneCopoeia's COVID-19 Coronavirus Research Tools products. Note: Spike-pseudotyped lentivirus is for research purposes only. It is not intended for diagnostic or treatment usage in humans.

Ordering information

Ordering information

Use the drop-down menus below to view products for lentivirus packaged with SARS-CoV-2 Spike protein.

For more information, please contact inquiry@genecopoeia.com.

SARS-CoV-2 Spike protein-pseudotyped lentivirus particles

GeneCopoeia's Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentivirus is available as either premade, in vitro grade or custom-produced in vivo grade particles.

Advantages
  • Accurate functional titers.  Functional titers are measured by FACS sorting of ACE2-expressing HEK293T cells infected with GFP-expressing particles.
  • Complete system. Premade, in vitro-grade purified particles can be used with GeneCopoeia's ACE2-expressing HEK293T cells as well as other ACE2-expressing cells. Kits for do-it-yourself packaging of Spike-pseudotyped lentivirus also available.
  • Application versatility.
    • Spike-pseudotyped lentivirus can be packaged for either in vitro grade or ultra-purified in vivo grade.
    • Viral particles can express either commonly-used markers such as GFP and luciferase, or most human, mouse, and rat ORFs in GeneCopoeia's >80 lentiviral transfer vector types.

 

    For more information, please contact inquiry@genecopoeia.com.

    Lentifect™ lentiviral particles packaged with full-length SARS-CoV-2 Spike protein (in vitro grade)

    Buy Catalog# Product Description Envelope tag Reporter / marker Vector Price
    Lentifect™ lentivirus pseudotyped with full-length Spike protein
    SP101-025 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length Spike-Pseudotyped Lentiviral Particles (25 µl x 1 vial) > 1×106 TU/ml* or > 1×108 RFU/ml* control lentiviral particles ready for transduction, 25 µl None eGFP/Puro, HLUC pReceiver-Lv201 $395
    SP101-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 full-length Spike-Pseudotyped Lentiviral Particles (25 µl x 4 vial) > 1×106 TU/ml* or > 1×108 RFU/ml* control lentiviral particles ready for transduction, 100 µl None eGFP/Puro, HLUC pReceiver-Lv201 $825
    Lentifect™ lentivirus without envelope protein (negative control)
    SP401-025 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 1 vial) > 1×106 TU/ml* lentiviral particles ready for transduction, 25 µl N/A eGFP/Puro, HLUC pReceiver-Lv201 $295
    SP401-100 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 4 vial) > 1×106 TU/ml* lentiviral particles ready for transduction, 100 µl N/A eGFP/Puro, HLUC pReceiver-Lv201 $725

    * The functional titers of SARS-CoV-2 Spike pseudotyped lentivirus are determined by FACS sorting of GFP positive ACE2-expressing ACE2/HEK293T cells (GeneCopoeia Cat.No. SL221) and by luciferase analysis (GeneCopoeia Cat.No. LF007, LF008, LF009) of transduced ACE2/HEK293T.

         

     

    Lentifect™ lentiviral particles packaged with SARS-CoV-2 Spike protein, 19 amino acid endoplasmic reticulum retention signal removed (in vitro grade)

     

    NOTE: Currently, there are few known significant differences in titer, or ability to infect HEK293T cells expressing ACE2, between lentiviruses pseudotyped with either the truncated (ER retention signal removed) or the full-length Spike protein. GeneCopoeia produces both versions of pseudotyped virus in case truncation of Spike protein causes post-infection functional differences, which are currently unknown.

     

     

    Buy Catalog # Product Description Envelope tag Reporter / marker Transfer vector Price
    Lentifect™ lentivirus pseudotyped with truncated Spike protein
    SP001-025 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (25 µl x 1 vial) > 1×106 TU/ml* or > 1×108 RFU/ lentiviral particles ready for transduction, 25 µl None eGFP/Puro, HLUC pReceiver-Lv201 $395
    SP001-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, ER retention signal removed (25 µl x 4 vial) > 1×106 TU/ml* or > 1×108 RFU/ml* lentiviral particles ready for transduction, 100 µl None eGFP/Puro, HLUC pReceiver-Lv201 $825
    Coming soon SP201-025 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, C-flag tagged and with ER retention signal removed (25 µl x 1 vial) > 1×106 TU/ml* or > 1×108 RFU/ml lentiviral particles ready for transduction, 25 µl C-Flag eGFP/Puro, HLUC pReceiver-Lv201 $395
    Coming soon SP201-100 HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, C-flag tagged and with ER retention signal removed (25 µl x 4 vial) > 1×106 TU/ml* or > 1×108 RFU/ml lentiviral particles ready for transduction, 100 µl C-Flag eGFP/Puro, HLUC pReceiver-Lv201 $825
    Lentifect™ lentivirus without envelope protein (negative control)
    SP401-025 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 1 vial) > 1×106 TU/ml* lentiviral particles ready for transduction, 25 µl N/A eGFP/Puro, HLUC pReceiver-Lv201 $295
    SP401-100 HLUC-Lv201 Firefly Luciferase Lentifect™ Lentiviral Particles without envelope protein (25 µl x 4 vial) > 1×106 TU/ml* lentiviral particles ready for transduction, 100 µl N/A eGFP/Puro, HLUC pReceiver-Lv201 $725

    * The functional titers of SARS-CoV-2 Spike pseudotyped lentivirus are determined by FACS sorting of GFP positive ACE2-expressing ACE2/HEK293T cells (GeneCopoeia Cat.No. SL221) and by luciferase analysis (GeneCopoeia Cat.No. LF007, LF008, LF009) of transduced ACE2/HEK293T.

         

     

    Lentifect™ lentiviral particles packaged with VSV-G glycoprotein (in vitro grade)

    Buy Catalog# Product Description Envelope tag Reporter / marker Vector Price
    LP464-025 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) > 1×108 TU/ml control lentiviral particles ready for transduction, 25 µl None eGFP/Puro, HLUC pReceiver-Lv201 $295
    LP464-100 HLUC-Lv201 Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 4 vial) > 1×108 TU/ml control lentiviral particles ready for transduction, 100 µl None eGFP/Puro, HLUC pReceiver-Lv201 $725

    Titers of standard VSV-G pseudotyped lentivirus are determined by RT-qPCR to determine physical titer (copies/ml) and extrapolated to functional titer (TU/ml).

         

     

    Lentifect™ SARS-CoV-2 Spike Protein Pseudotyped Lentivirus Particle Kit (in vitro grade)

    Kit includes:

    • Lentifect™ SARS-CoV-2 Spike protein-pseudotyped lentiviral particles
    • Lentifect™ standard VSV-G-pseudotyped lentiviral particles
    • Lentifect™ lentiviral particles packaged without envelope protein (negative control)
    • GeneCopoeia's ACE2-expressing HEK293T cell line

     

    Buy Catalog # Product Description Envelope tag Reporter / marker Vector Price, Academic Price, Industry
    LT013 Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particle Kit, ER retention signal removed SARS-CoV-2 spike-pseudotyped lentivirus (25 µl x 4 vial), lentivirus without envelope protein negative control  (25 µl x 1 vial), standard VSV-G lentivirus (25 µl x 1 vial), ACE2-expressing HEK293T cells (2 x 106 cells x 1 vial) None eGFP/Puro, HLUC pReceiver-Lv201 $2820 $4820
    Coming soon LT014 Lentifect™ SARS-CoV-2 Spike-Pseudotyped Lentiviral Particle Kit SARS-CoV-2 spike-pseudotyped lentivirus (25 µl x 4 vial), lentivirus without envelope protein negative control  (25 µl x 1 vial), standard VSV-G lentivirus (25 µl x 4 vial), ACE2-expressing HEK293T cells (2 x 106 cells x 1 vial) None eGFP/Puro, HLUC pReceiver-Lv201 $2820 $4820

     

         

     

    For more information or to request a quote, please contact inquiry@genecopoeia.com.

    Packaging kits for pseudotyping lentivirus with SARS-CoV-2 Spike protein

      GeneCopoeia manufactures kits that allow researchers to produce SARS-CoV-2 Spike-pseudotyped lentivirus, which you can order from the table below.

       

      NOTE: Currently, there are few known significant differences in titer, or ability to infect HEK293T cells expressing ACE2, between lentiviruses pseudotyped with either the truncated (ER retention signal removed) or the full-length Spike protein. GeneCopoeia produces both versions of pseudotyped virus packaging kits in case truncation of Spike protein causes  post-infection functional differences, which are currently unknown.

       

       

      Buy Catalog# Product Description Enveope tag Price
      Kits for packaging lentivirus pseudotyped with truncated Spike protein (ER retention signal removed)

      LT021

      Lenti-Pac SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit, ER retention signal removed (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent None $745

      LT022

      Lenti-Pac SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit, ER retention signal removed (40 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent None $1235
      Kits for packaging lentivirus pseudotyped with full-length Spike protein
      Coming soon

      LT011

      Lenti-Pac SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent None $745
      Coming soon

      LT012

      Lenti-Pac SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids for inserting Spike protein into the viral envelope, eGFP control clone and EndoFectin™ Lenti Transfection Reagent None $1235

       

        User Manual    
         

       

      For more information or to request a quote, please contact inquiry@genecopoeia.com.

      Cell lines expressing human ACE2

      GeneCopoeia carries a HEK293T cell line expressing ACE2, the receptor needed for SARS-CoV-2 infection in humans. This cell line is great for use with Spike protein pseudotyped lentivirus for vaccine development, studies of COVID-19 neutralizing antibodies, and more.

      You can order this cell line from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

      In addition to the products below, custom stable cell lines, are also available. For more information, please contact inquiry@genecopoeia.com.

      Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
      SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $1395 $3995

       

        Warranty statement and limited use license   MSDS
       

       

      For more information or to request a quote, please contact inquiry@genecopoeia.com.

      Firefly luciferase assay kits

      Luc-Pair™ Firefly Luciferase HS Assay Kit 

      Buy Catalog# Product Description Price
      LF007 Luc-Pair™ Firefly Luciferase HS Assay Kit (100rxns) High sensitivity for detecting the activity of Firefly luciferase. $77
      LF008 Luc-Pair™ Firefly Luciferase HS Assay Kit (300rxns) $125
      LF009 Luc-Pair™ Firefly Luciferase HS Assay Kit (1000rxns) $375
      LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $40

        User manual MSDS