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GeneHero™ CRISPR-Cas9 stable cell lines

GeneCopoeia's stable cell lines constitutively expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications with high efficiency.

The GeneHero™ Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The S. pyogenes CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites, or randomly via lentiviral-mediated transduction.

The CRISPR Cas9 nuclease, in combination with single guide RNAs (sgRNAs), is widely used to create targeted genomic modifications, from gene knockouts, mutagenesis, fusion tagging, and more, in eukaryotic cells and animal models. Stable Cas9 integration ensures robust and consistent expression of the CRISPR Cas9 nuclease.

Advantages

Don't see the Cas9 stable cell line you want? We also offer custom cell line service. Please contact us at inquiry@genecopoeia.com, or call 866-360-9531 or 301-762-0888 for a quote.

 

 

Order pre-made Cas9-expressing stable cell lines

Choose your human, mouse, or rat Cas9-expressing stable cell line from the list below. You can also find Safe Harbor Cas9 knock-in kits, for do-it-yourself engineering of the Cas9-expressing stable cell line of your choice. In addition, you can purchase one of our IndelCheck™ insertion/deletion detection kits, for efficient validation of CRISPR sgRNAs and TALENs, and for screening for CRISPR- or TALEN-modified cell lines

Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
Human cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL502 HEK293T Human cell line HEK293T stably expressing Cas9 from AAVS1 site Embryonic kidney Puro $945 $2695
SL554 HEK293 Human cell line HEK293 stably expressing Cas9 from AAVS1 site, with loxP Embryonic kidney Hygro $945 $2695
SL501 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from AAVS1 site Lung cancer Puro $1395 $3995
SL533 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL504 A549 Human cell line A549 stably expressing Cas9 from AAVS1 site Lung cancer Hygro $1395 $3995
SL529 H1975 Human cell line H1975 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL536 NCI-H1437 Human cell line NCI-H1437 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL561 NCI-H661 Human cell line NCI-H661 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL578NEW HCC827 Human cell line HCC827 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL503 HeLa Human cell line HeLa stably expressing Cas9 from AAVS1 site Cervical cancer Hygro $1395 $3995
SL514 MCF-7 Human cell line MCF-7 stably expressing Cas9 from AAVS1 site Breast cancer Hygro $1395 $3995
SL524 MCF-7 Human cell line MCF-7 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL515 MDA-MB-231 Human cell line MDA-MB-231 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL516 MDA-MB-468 Human cell line MDA-MB-468 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL517 T47D Human cell line T47D stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL535 SK-BR-3 Human cell line SK-BR-3 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL544 HCC70 Human cell line HCC70 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL547 DU4475 Human cell line DU4475 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL558 HCC1428 Human cell line HCC1428 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL562NEW CAMA-1 Human cell line CAMA-1 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL563NEW HCC38 Human cell line HCC38 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL574NEW AU-565 Human cell line AU-565 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL575NEW HCC1500 Human cell line HCC1500 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL518 HepG2 Human cell line HepG2 stably expressing Cas9 from AAVS1 site Liver cancer Hygro $1395 $3995
SL522 SNU-475 Human cell line SNU-475 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL530 SNU-449 Human cell line SNU-449 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL531 PLC/PRF/5 Human cell line PLC/PRF/5 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL537 SNU-387 Human cell line SNU-387 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL538 SNU-423 Human cell line SNU-423 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL543 C3A Human cell line C3A stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL557 SK-HEP-1 Human cell line SK-HEP-1 stably expressing Cas9 from random site Liver/ascites cancer Hygro $1395 $3995
SL520 AGS Human cell line AGS stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL526 SNU-1 Human cell line SNU-1 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL527 KATOIII Human cell line KATOIII stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL545 SNU-16 Human cell line SNU-16 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL565NEW NCI-N87 Human cell line NCI-N87 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL521 BXPC-3 Human cell line BXPC-3 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL528 HPAF-II Human cell line HPAF-II stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL540 CFPAC-1 Human cell line CFPAC-1 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL570NEW Panc 10.05 Human cell line Panc 10.05 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL523 HT-29 Human cell line HT-29 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL525 LoVo Human cell line LoVo stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL539 RKO Human cell line RKO stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL541 T84 Human cell line T84 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL542 COLO 205 Human cell line COLO 205 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL551 SNU-C1 Human cell line SNU-C1 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL572NEW HCT116 Human cell line HCT116 stably expressing Cas9 from AAV1 site Colon cancer Hygro $1395 $3995
SL532 LS411N Human cell line LS411N stably expressing Cas9 from random site Caecum cancer Hygro $1395 $3995
SL583NEW Granta-519 Human cell line Granta-519 stably expressing Cas9 from random site Blood cancer Hygro $1395 $3995
SL546 U-2-OS Human cell line U-2 OS stably expressing Cas9 from random site Bone cancer Hygro $1395 $3995
SL552 K562 Human cell line K562 stably expressing Cas9 from random site Bone marrow cancer Hygro $1395 $3995
SL555 Jurkat Human T lymphocyte cell line Jurkat stably expressing Cas9 from random site Acute T cell leukemia Hygro $1395 $3995
SL548 DU145 Human cell line DU145 stably expressing Cas9 from random site Prostate cancer Hygro $1395 $3995
SL550 SH-SY5Y Human cell line SH-SY5Y stably expressing Cas9 from random site Neuroblastoma Hygro $1395 $3995
SL560 T24 Human cell line T24 stably expressing Cas9 from random site Bladder cancer Hygro $1395 $3995
SL566 HK2 Human cell line HK2 stably expressing Cas9 from random site Immortalized kidney cell line Hygro $1395 $3995
SL577NEW 786-O Human cell line 786-O stably expressing Cas9 from random site Kidney cancer Hygro $1395 $3995
SL576NEW 1321N1 Human cell line 1321N1 stably expressing Cas9 from random site Brain astrocytoma Hygro $1395 $3995
SL512NEW HT1080 Human cell line HT1080 stably expressing Cas9 from AAVS1 Fibrosarcoma Hygro $1395 $3995
SL580NEW SK-OV-3 Human cell line SK-OV-3 stably expressing Cas9 from random site Ovary Hygro $1395 $3995
Human cell lines stably expressing high-fidelity S. pyogenes Cas9 (SpCas9-HF1)
SL553 HEK293 Human cell line HEK293 stably expressing SpCas9-HF1 from AAVS1 site Embryonic kidney Hygro $1795 $4995
Mouse cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL509 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Hygro $1395 $3995
SL559 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site, with loxP Neuroblastoma Hygro $1395 $3995
SL510 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Puro $1395 $3995
SL511 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Neo $1395 $3995
SL564 C2C12 Mouse cell line C2C12 stably expressing Cas9 from random site Muscle (myoblast) Hygro $1395 $3995
SL567 BA/F3 Mouse cell line BA/F3 stably expressing Cas9 from random site Lymph Hygro $1395 $3995
SL569NEW NIH-3T3 Mouse cell line NIH-3T3 stably expressing Cas9 from random site Embryo fibroblast Hygro $1395 $3995
SL581NEW 4T1 Mouse cell line 4T1 stably expressing Cas9 from random site Mammary gland Hygro $1395 $3995
SL582NEW CT26 Mouse cell line CT26 stably expressing Cas9 from random site Colon Hygro $1395 $3995
Mouse cell lines stably expressing high-fidelity S. pyogenes Cas9 (SpCas9-HF)
SL571 Neuro2a Mouse cell line Neuro2a stably expressing SpCas9-HF from ROSA26 site Neuroblastoma Hygro $1795 $4995
Rat cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL534 C6 Rat cell line C6 stably expressing Cas9 from random site Glioma Hygro $1395 $3995

  Warranty statement and limited use license   MSDS
 

 

Note: GeneCopoeia also offers custom-built CRISPR cell line services. To inquire, please contact inquiry@genecopoeia.com.

 

 

 

Control Lentivirus expressing sgRNA

Buy sgRNA
Target
Cat # Promoter Selection Marker/Reporter Gene Titer Volume
GFP LPP-OCP001145-LvSG03-1-50-A00 U6 Puromycin / mCherry 108 TU/ml 50 µl
HUWE LPP-HUWE-LvSG03-A00 U6 Puromycin / mCherry 108 TU/ml 50 µl
EMX1 LPP-EMX1-LvSG03-A00 U6 Puromycin / mCherry 108 TU/ml 50 µl

Lentiviruses carrying functional validated sgRNA targeting either GFP, HUWE or EMX1 are available as positive controls for the premade stable Cas9 cell lines. Simply transduce the control sgRNA-lentivirus to the Cas9 cell line, the infected cells can be selected by mCherry fluorescence and puromycin resistance, and editing of the target genes can be verified with either T7 Endonulease I assay or DNA sequencing.

T7EI assay for Cas9 cell linePlasmid for Cas9 cell line positive control lentivirus

Figure Left: Demonstration of stable Cas9 activity in a cell line. Human H1299 cells stably expressing Cas9 nuclease from the AAVS1 Safe Harbor site were transduced with HUWE sgRNA-lentivirus (Cat # LPP-HUWE-LvSG03-A00). PCR products flanking the sgRNA target site were generated, denatured, reannealed, and treated with (+) or without (-) T7 Endonuclease I, which cleaves mismatched DNA. The presence of two bands below the uncut band at the correct sizes indicates that Cas9 is active in these cells. Right: Lentiviral plasmid carrying target sgRNA (either GFP, HUWE or EMX1) used for lentivirus packaging.

 

 

Applications

  • High-throughput knockout screening with many sgRNAs, either individually or in pools. Ideal for drug target discovery (Figure 3).
  • Convenient validation of several drug target candidates.
  • Validation of sgRNA cleavage activity in a fast-growing, easy-to-transfect or transduce model cell line, either prior to transfection/transduction of your cell line, or to troubleshoot sgRNAs with little or no cleavage activity.
  • Applications requiring inducible genome modification.

 

Figure 3. Example application of Cas9-expressing stable cell lines Use of the GeneHero™ Cas9 stable cell lines with CRISPR sgRNA libraries for drug target discovery. A. Pooled screen. Cas9-expressing cells infected with each sgRNA library pool are screened for the desired readout. Pooled cells are subjected to Sanger sequencing for individual sgRNAs, or deep sequencing to look for over- or under-representation of individual sgRNAs. B. Knockout screen using arrayed sgRNAs. Cas9-expressing cells are infected with individual sgRNA lentiviruses. Wells are screened for the readout of interest. Individual sgRNAs corresponding to the phenotype of interest are already known without sequencing.

Cas9 cell line validation by T7 endonuclease I assay

Figure 4. sgRNA targeting to EMX1 or HUWE gene were transduced into Cas9 cell line by lentiviral particals. EMX1 or HUWE gene was cut by Cas9 expressed inside the cells and repaired through NHEJ with mutation. The mutations were recognized and cut by T7 Endonuclease I, resulting in shorter DNA fragments (marked with red asterisks).

 

Technical note

Cas9 stable cell line publications

2018

2017