GeneHero™ CRISPR-Cas9 stable cell lines

GeneCopoeia’s stable cell lines constitutively expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications with high efficiency.
The GeneHero™ Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The S. pyogenes CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites, or randomly via lentiviral-mediated transduction.
The CRISPR Cas9 nuclease, in combination with single guide RNAs (sgRNAs), is widely used to create targeted genomic modifications, from gene knockouts, mutagenesis, fusion tagging, and more, in eukaryotic cells and animal models. Stable Cas9 integration ensures robust and consistent expression of the CRISPR Cas9 nuclease.
 
Advantages
 
Don’t see the Cas9 stable cell line you want? We also offer custom cell line service. Please contact us at inquiry@genecopoeia.com, or call 301-762-0888 for a quote.
Stable Cas9 cell lines

Order pre-made Cas9-expressing stable cell lines

Choose your human, mouse, or rat Cas9-expressing stable cell line from the list below. You can also find Safe Harbor Cas9 knock-in kits, for do-it-yourself engineering of the Cas9-expressing stable cell line of your choice. In addition, you can purchase one of our IndelCheck™ insertion/deletion detection kits, for efficient validation of CRISPR sgRNAs and TALENs, and for screening for CRISPR- or TALEN-modified cell lines
To obtain the datasheet for each cell line, please click on the designated catalog number.
Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
Human cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL502 HEK293T Human cell line HEK293T stably expressing Cas9 from AAVS1 site Embryonic kidney Puro $945 $2695
SL554 HEK293 Human cell line HEK293 stably expressing Cas9 from AAVS1 site, with loxP Embryonic kidney Hygro $945 $2695
SL501 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from AAVS1 site Lung cancer Puro $1395 $3995
SL533 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL504 A549 Human cell line A549 stably expressing Cas9 from AAVS1 site Lung cancer Hygro $1395 $3995
SL529 H1975 Human cell line H1975 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL536 NCI-H1437 Human cell line NCI-H1437 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL561 NCI-H661 Human cell line NCI-H661 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL578 HCC827 Human cell line HCC827 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL503 HeLa Human cell line HeLa stably expressing Cas9 from AAVS1 site Cervical cancer Hygro $1395 $3995
SL514 MCF-7 Human cell line MCF-7 stably expressing Cas9 from AAVS1 site Breast cancer Hygro $1395 $3995
SL515 MDA-MB-231 Human cell line MDA-MB-231 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
  SL516 backorder MDA-MB-468 Human cell line MDA-MB-468 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL517 T47D Human cell line T47D stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
  SL535 backorder SK-BR-3 Human cell line SK-BR-3 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
  SL544 backorder HCC70 Human cell line HCC70 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL547 DU4475 Human cell line DU4475 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL558 HCC1428 Human cell line HCC1428 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL562 CAMA-1 Human cell line CAMA-1 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL563 HCC38 Human cell line HCC38 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL574 AU-565 Human cell line AU-565 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL531 PLC/PRF/5 Human cell line PLC/PRF/5 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL538 SNU-423 Human cell line SNU-423 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL543 C3A Human cell line C3A stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL557 SK-HEP-1 Human cell line SK-HEP-1 stably expressing Cas9 from random site Liver/ascites cancer Hygro $1395 $3995
SL520 AGS Human cell line AGS stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL526 SNU-1 Human cell line SNU-1 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL527 KATOIII Human cell line KATOIII stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL545 SNU-16 Human cell line SNU-16 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
  SL565 backorder NCI-N87 Human cell line NCI-N87 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
  SL521 backorder BXPC-3 Human cell line BXPC-3 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL528 HPAF-II Human cell line HPAF-II stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL540 CFPAC-1 Human cell line CFPAC-1 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL570 Panc 10.05 Human cell line Panc 10.05 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL523 HT-29 Human cell line HT-29 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL525 LoVo Human cell line LoVo stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL539 RKO Human cell line RKO stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL541 T84 Human cell line T84 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL542 COLO 205 Human cell line COLO 205 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL572 HCT116 Human cell line HCT116 stably expressing Cas9 from AAVS1 site Colon cancer Hygro $1395 $3995
SL532 LS411N Human cell line LS411N stably expressing Cas9 from random site Caecum cancer Hygro $1395 $3995
  SL583 backorder Granta-519 Human cell line Granta-519 stably expressing Cas9 from random site Blood cancer Hygro $1395 $3995
SL546 U-2-OS Human cell line U-2 OS stably expressing Cas9 from random site Bone cancer Hygro $1395 $3995
SL552 K562 Human cell line K562 stably expressing Cas9 from random site Bone marrow cancer Hygro $1395 $3995
SL555 Jurkat Human T lymphocyte cell line Jurkat stably expressing Cas9 from random site Acute T cell leukemia Hygro $1395 $3995
SL548 DU145 Human cell line DU145 stably expressing Cas9 from random site Prostate cancer Hygro $1395 $3995
SL550 SH-SY5Y Human cell line SH-SY5Y stably expressing Cas9 from random site Neuroblastoma Hygro $1395 $3995
  SL560 backorder T24 Human cell line T24 stably expressing Cas9 from random site Bladder cancer Hygro $1395 $3995
SL566 HK2 Human cell line HK2 stably expressing Cas9 from random site Immortalized kidney cell line Hygro $1395 $3995
SL577 786-O Human cell line 786-O stably expressing Cas9 from random site Kidney cancer Hygro $1395 $3995
SL576 1321N1 Human cell line 1321N1 stably expressing Cas9 from random site Brain astrocytoma Hygro $1395 $3995
SL584 U87-MG Human cell line U87-MG stably expressing Cas9 from AAVS1 site Brain glioma Hygro $1395 $3995
SL512 HT1080 Human cell line HT1080 stably expressing Cas9 from AAVS1 Fibrosarcoma Hygro $1395 $3995
SL580 SK-OV-3 Human cell line SK-OV-3 stably expressing Cas9 from random site Ovarian cancer Hygro $1395 $3995
Human cell lines stably expressing high-fidelity S. pyogenes Cas9 (SpCas9-HF1)
SL553 HEK293 Human cell line HEK293 stably expressing SpCas9-HF1 from AAVS1 site Embryonic kidney Hygro $1795 $4995
Mouse cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL509 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Hygro $1395 $3995
SL559 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site, with loxP Neuroblastoma Hygro $1395 $3995
SL510 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Puro $1395 $3995
SL511 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Neo $1395 $3995
SL564 C2C12 Mouse cell line C2C12 stably expressing Cas9 from random site Muscle (myoblast) Hygro $1395 $3995
SL567 BA/F3 Mouse cell line BA/F3 stably expressing Cas9 from random site Plasma cell cancer Hygro $1395 $3995
SL569 NIH-3T3 Mouse cell line NIH-3T3 stably expressing Cas9 from random site Embryo fibroblast Hygro $1395 $3995
SL581 4T1 Mouse cell line 4T1 stably expressing Cas9 from random site Mammary gland Hygro $1395 $3995
SL582 CT26 Mouse cell line CT26 stably expressing Cas9 from random site Colon Hygro $1395 $3995
Mouse cell lines stably expressing high-fidelity S. pyogenes Cas9 (SpCas9-HF1)
SL571 Neuro2a Mouse cell line Neuro2a stably expressing SpCas9-HF1 from ROSA26 site Neuroblastoma Hygro $1795 $4995
Rat cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL534 C6 Rat cell line C6 stably expressing Cas9 from random site Glioma Hygro $1395 $3995
Immortalized cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL586 hSMC Immortalized cell line hSMC stably expressing CRISPR Cas9 from random site Muscle Hygro $1395 $3995
SL587 hGBE Immortalized cell line hGBE stably expressing CRISPR Cas9 from random site Epithelial Hygro $1395 $3995
Warranty statement and limited use license MSDS
 

Control Lentivirus expressing sgRNA

Buy sgRNA Target Cat # Promoter Selection Marker/Reporter Gene Titer Volume
eGFP LP606-050 U6 Puromycin / mCherry 108 TU/ml 50 µl
copGFP LP629-050 U6 Puromycin / mCherry 108 TU/ml 50 µl
HUWE LP607-050 U6 Puromycin / mCherry 108 TU/ml 50 µl
EMX1 LP608-050 U6 Puromycin / mCherry 108 TU/ml   50 µl

Lentiviruses carrying functional validated sgRNA targeting either GFP, HUWE or EMX1 are available as positive controls for the premade stable Cas9 cell lines. Simply transduce the control sgRNA-lentivirus to the Cas9 cell line, the infected cells can be selected by mCherry fluorescence and puromycin resistance, and editing of the target genes can be verified with either T7 Endonulease I assay or DNA sequencing.
T7EI assay for Cas9 cell line Plasmid for Cas9 cell line positive control lentivirus
Figure Left: Demonstration of stable Cas9 activity in a cell line. Human H1299 cells stably expressing Cas9 nuclease from the AAVS1 Safe Harbor site were transduced with HUWE sgRNA-lentivirus (Cat # LPP-HUWE-LvSG03-A00). PCR products flanking the sgRNA target site were generated, denatured, reannealed, and treated with (+) or without (-) T7 Endonuclease I, which cleaves mismatched DNA. The presence of two bands below the uncut band at the correct sizes indicates that Cas9 is active in these cells. Right: Lentiviral plasmid carrying target sgRNA (either GFP, HUWE or EMX1) used for lentivirus packaging.