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COVID-19 Coronavirus Plasmid Clones

General information

GeneCopoeia is the industry leader in expression-ready ORF cDNA clones, and we also carry reporter plasmid clones carrying gene promoters and 3' UTR microRNA (miRNA) binding site targets. OmicsLink™ SARS-CoV-2-related ORF cDNA plasmid clones can be constructed in any of our more than 250 ready-to-go vector types expressing both SARS-CoV-2 viral proteins as well as host proteins, which you can select using the drop-down menus below.

OmicsLink™ SARS-CoV-2-related ORF cDNA plasmid clones are part of GeneCopoeia's COVID-19 Coronavirus Research Tools products.

Note: SARS-CoV-2-related ORF cDNA plasmid clones are for research purposes only. They are not intended for diagnostic or treatment usage in humans.

We also have OmicsLink™ ORF cDNA plasmid clones for the related virus SARS-CoV-1, which caused the 2002-2004 SARS outbreak.

COVID-19 ORF cDNA plasmid clones-viral Spike proteins

Order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral Spike proteins

To order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral Spike proteins in one of our more than 250 non-viral and lentiviral vector types, select the product ID from the table below and contact inquiry@genecopoeia.com.

To view the Spike mutations in each Spike variant, refer to the table "Spike variant mutational profiles" below.

Product ID Protein accession Symbol Alias Spike genotype Description
NV219 YP_009724390.1 S GU280_gp02 WT surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene (codon optimized)
NV236 YP_009724390.1 S GU280_gp02 D614G surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G mutation (codon optimized)
NV237 YP_009724390.1 S GU280_gp02 N439K surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with N439K mutation (codon optimized)
NV238 YP_009724390.1 S GU280_gp02 D614G, N439K surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with N439K, D614G mutations (codon optimized)
NV239 YP_009724390.1 S GU280_gp02 D614G, E501Y surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, E501Y mutations (codon optimized)
NV240 YP_009724390.1 S GU280_gp02 D614G, P681H surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, P681H mutations (codon optimized)
NV241 YP_009724390.1 S GU280_gp02 D614G, Hv69-90deletion surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, Hv69-90deletion mutations (codon optimized)
NV242 YP_009724390.1 S GU280_gp02

D614G,

U.K. B.1.1.7

 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, U.K. B.1.1.7 mutations (codon optimized)
NV244 YP_009724390.1 S GU280_gp02

D614G,

South Africa B.1.351

 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, South Africa B.1.351 mutations (codon optimized)
NV245 YP_009724390.1 S GU280_gp02

D614G,

Brazil P.1

 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with D614G, Brazil P.1 mutations (codon optimized)
NV247 YP_009724390.1 S GU280_gp02 California B.1.427/B.1.429 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with California B.1.427/B.1.429 mutations (codon optimized)
NV248 YP_009724390.1 S GU280_gp02 India B.1.617 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene with India B.1.617 mutations (codon optimized)
NV220 YP_009724390.1 S1 GU280_gp02 WT surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide (codon optimized)
NV221 YP_009724390.1 S2 GU280_gp02 WT surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)
NV222 YP_009724390.1 RBD GU280_gp02 WT Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)

 

 

Spike variant mutational profiles

At the time of product introduction, GeneCopoeia's R&D team used the GISAID database to determine the most frequently-occurring mutations in the Spike proteins from each variant to incorporate into our Spike-expressing ORF clones. These mutational profiles are indicated below. Note that each variant can have different sub-lineages, which could contain a slightly different repertoire of mutations from the variants that we have made available. If you would like SARS-CoV-2 Spike-expressing ORF clones with a different set of mutations than we have currently available. please contact inquiry@genecopoeia.com for a custom project.

 

 

 

 

 

 

 

 

 

SARS-CoV-2 variant Spike mutations
Wild type (product IDs NV219, NV220, NV221, NV222) None
D614G (product ID NV236) D614G
N439K (product ID NV237) N439K
N439K & D614G (product ID NV238) N439K, D614G
N439K & N501Y (product ID NV239) N439K, N501Y
D614G & P681H (product ID NV240) D614G, P681H
D614G & Hv69-90deletion (product ID NV241) Δ69-70, D614G
B.1.1.7 (first identified in the U.K.; product ID NV242) Δ69-70, Δ144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
B.1.351 (first identified in South Africa; product ID NV244) L18F, D80A, D215G，Δ242-244, K417N, E484K, N501Y, D614G, A701V
P.1 (first identified in Brazil; product ID NV245) L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F

 

COVID-19 ORF cDNA plasmid clones-viral non-Spike proteins

Order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral proteins 

To order OmicsLink™ ORF cDNA clones expressing SARS-CoV-2 viral proteins in one of our more than 250 non-viral and lentiviral vector types, select the product ID from the table below and contact inquiry@genecopoeia.com.

Product ID Protein accession Symbol Alias Description
NV201 (please inquire) YP_009724389.1 orf1ab GU280_gp01 orf1ab polyprotein [Severe acute respiratory syndrome coronavirus 2}, full-length gene
NV202 YP_009725297.1 orf1ab GU280_gp01 leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV203 YP_009725298.1 orf1ab GU280_gp01 nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV204 YP_009725299.1 orf1ab GU280_gp01 nsp3n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV205 YP_009725300.1 orf1ab GU280_gp01 nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV206 YP_009725301.1 orf1ab GU280_gp01 3C-like proteinase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV207 YP_009725302.1 orf1ab GU280_gp01 nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV208 YP_009725303.1 orf1ab GU280_gp01 nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV209 YP_009725304.1 orf1ab GU280_gp01 nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV210 YP_009725305.1 orf1ab GU280_gp01 nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV211 YP_009725306.1 orf1ab GU280_gp01 nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV212 YP_009725307.1 orf1ab GU280_gp01 RNA-dependent RNA polymerase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV213 YP_009725308.1 orf1ab GU280_gp01 helicase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV214 YP_009725309.1 orf1ab GU280_gp01 3′-to-5′ exonuclease [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV215 YP_009725310.1 orf1ab GU280_gp01 endoRNAse [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV216 YP_009725311.1 orf1ab GU280_gp01 2′-O-ribose methyltransferase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV217 (please inquire) YP_009725295.1 orf1a GU280_gp01 orf1a polyprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV218 YP_009725312.1 orf1a GU280_gp01 produced by pp1a only [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV223 YP_009724391.1 ORF3a GU280_gp03 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV224 YP_009724392.1 E GU280_gp04 envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV225 YP_009724393.1 M GU280_gp05 membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV226 YP_009724394.1 ORF6 GU280_gp06 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV227 YP_009724395.1 ORF7a GU280_gp07 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV228 YP_009725318.1 ORF7b

GU280_gp08

 ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV229 YP_009724396.1 ORF8 GU280_gp09 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV230 YP_009724397.2 N GU280_gp10 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV231 YP_009725255.1 ORF10 GU280_gp11 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene

 

 

*Note: Mature peptides are produced as a result of proteolytic processing of larger precursor proteins. To enable expression of these proteins in cells, an ATG has been added to the 5' end of the ORF sequence.

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

 

COVID-19 ORF cDNA plasmid clones-host proteins

ACE2

You can purchase ORF cDNA clones expressing human host proteins that SARS-CoV-2 interacts with, including ACE2, which encodes the receptor for SARS-CoV-2 on respiratory epithelial cells, in more than 250 non-viral and lentiviral vector types. Either view all standard clones using the buttons below, or choose human clones in several popular vector types in the table. If you don't see what you need here, please contact inquiry@genecopoeia.com.

 

View all ACE2 ORF cDNA clones for human, mouse, and rat 

 

 

Order human ACE2 ORF cDNA clones in popular vector types

 

 

Clones in yellow shading are available for next-day shipping in bacterial stock format.**

 

Buy Catalog# Vector Type Promoter Tag / Reporter / Other Features Mammalian Selection Markers Vector Name List Price Discount price
ORF cDNA plasmid clones expressing human ACE2  

EX-U1285-M02-B

(Bacterial stock)

 Mammalian CMV Untagged Neomycin pReceiver-M02 $779 $434

EX-U1285-M02-10

(10 ug purified plasmid)

 Mammalian CMV Untagged Neomycin pReceiver-M02 $829 $484

EX-U1285-M98-B

(Bacterial stock)

 Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $779 $434

EX-U1285-M98-10

(10 ug purified plasmid)

 Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $829 $484

EX-U1285-M35

(10 ug purified plasmid)

 Mammalian CMV C-Flag Neomycin pReceiver-M35 $779 $779

EX-U1285-Lv105-B

(Bacterial stock)

 Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $829 $484

EX-U1285-Lv105-10

(10 ug purified plasmid)

 Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $879 $534

EX-U1285-Lv242-B

(Bacterial stock)

 Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $879 $484

EX-U1285-Lv242-10

(10 ug purified plasmid)

 Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $929 $534

GC-U1285-B

(Bacterial stock)

 Gateway PLUS shuttle — Untagged — pShuttle™ $779 $484

GC-U1285-10

(10 ug purified plasmid)

 Gateway PLUS shuttle — Untagged — pShuttle™ $829 $534

 

 

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

** Next-day shipping is available for NextDay ORF clones in bacterial stock format. Lead times for NextDay ORF clones in purified plasmid is ~3-5 days. Orders for NextDay ORF clones must be received by 12PM EST Mon-Thurs in order to be processed and shipped the next day.

TMPRSS2

You can purchase ORF cDNA clones expressing human host proteins that SARS-CoV-2 interacts with, including TMPRSS2, a serine protease required for S protein priming, in more than 250 non-viral and lentiviral vector types. Either view all standard clones using the buttons below, or choose human clones in several popular vector types in the table. If you don't see what you need here, please contact inquiry@genecopoeia.com.

View all TMPRSS2 ORF cDNA clones for human, mouse, and rat 

 

 

Order human TMPRSS2 ORF cDNA clones in popular vector types

 

 

Clones in yellow shading are available for next-day shipping in bacterial stock format.**

 

Buy Catalog# Vector Type Promoter Tag / Reporter / Other Features Mammalian Selection Markers Vector Name List Price Discount price
ORF cDNA plasmid clones expressing human TMPRSS2  

EX-Z7591-M02-B

(Bacterial stock)

 Mammalian CMV Untagged Neomycin pReceiver-M02 $399 $300

EX-Z7591-M02-10

(10 ug purified plasmid)

 Mammalian CMV Untagged Neomycin pReceiver-M02 $449 $350

EX-Z7591-M98-B

(Bacterial stock)

 Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $399 $300

EX-Z7591-M98-10

(10 ug purified plasmid)

 Mammalian CMV C-eGFP(monomeric) Neomycin pReceiver-M98 $449 $350

EX-Z7591-M35

(10 ug purified plasmid)

 Mammalian CMV C-Flag Neomycin pReceiver-M35 $399 $399

EX-Z7591-Lv105-B

(Bacterial stock)

 Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $449 $350

EX-Z7591-Lv105-10

(10 ug purified plasmid)

 Lentiviral CMV Untagged Puromycin pReceiver-Lv105 $499 $400

EX-Z7591-Lv242-B

(Bacterial stock)

 Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $499 $350

EX-Z7591-Lv242-10

(10 ug purified plasmid)

 Lentiviral CMV C-Avi-FLAG Puromycin pReceiver-Lv242 $549 $400

GC-Z7591-B

(Bacterial stock)

 Gateway PLUS shuttle — Untagged — pShuttle™ $399 $350

GC-Z7591-10

(10 ug purified plasmid)

 Gateway PLUS shuttle — Untagged — pShuttle™ $449 $400

 

 

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

** Next-day shipping is available for NextDay ORF clones in bacterial stock format. Lead times for NextDay ORF clones in purified plasmid is ~3-5 days. Orders for NextDay ORF clones must be received by 12PM EST Mon-Thurs in order to be processed and shipped the next day.

Cyclophilin A

 

The cyclophilins are a family of proline isomerases that function to aid in protein folding. Cyclophilins bind to the immunosuppressive drug cyclosporin A (CsA) and help mediate CsA's pharmacological function. While there is no published evidence yet that cyclophilins are involved in the pathogenesis of SARS-CoV-2, several groups have shown that CsA can inhibit several viruses, including the virus that caused the 2002-2004 SARS pandemic, SARS-CoV, in a cyclophilin A (CypA)-dependent fashion (reviewed in Tanaka, Y., et al., 2013. Viruses 5, 1,250). Further, CypA interacts with SARS-CoV Non-structural protein 1 (Nsp1) as demonstrated in a yeast 2-hybrid screen (Pfefferle, E., et al.. 2011; PLOS Pathogens doi:  doi:10.1371/journal.ppat.1002331). SARS-CoV is highly similar to SARS-CoV-2, and, in fact, both viruses use ACE2 as their cellular receptor. Therefore, it is reasonable to include CypA in investigations of a potential role for CypA in SARS-CoV-2 pathogenesis.

GeneCopoeia provides non-viral and lentiviral ORF cDNA clones in 250+ vector types for CYPA in human, mouse, and rat.

 

View all human cyclophilin A ORF cDNA clones

Humans have two known isoforms of cyclophilin A (PPIA). You can order human cyclophilin ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below

 

Product ID Accession Symbol Alias Description
F0323 NM_021130.4 PPIA CYPA, CYPH, HEL-S-69p Homo sapiens peptidylprolyl isomerase A (PPIA), transcript variant 1, mRNA.
T2782 NM_001300981.1 PPIA CYPA, CYPH, HEL-S-69p Homo sapiens peptidylprolyl isomerase A (PPIA), transcript variant 2, mRNA.

 

View all mouse M. musculus cyclophilin A ORF cDNA clones

Mouse M. musculus has only one known isoform of cyclophilin A (PPIA). You can order mouse cyclophilin A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

 

Product ID Accession Symbol Alias Description
Mm17577 NM_008907.1 Ppia 2700098C05, Cphn, CyP-18, CypA Mus musculus peptidylprolyl isomerase A (Ppia), mRNA.

 

View all rat R. norvegicus cyclophilin A ORF cDNA clones

Rat R. norvegicus has only one known isoform of cyclophilin A (PPIA). You can order rat cyclophilin A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

 

Product ID Accession Symbol Alias Description
Rn24256 NM_017101.1 Ppia CYCA, CyP-A Rattus norvegicus peptidylprolyl isomerase A (Ppia), mRNA.

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

FKBP1A

 

FK506 binding proteins (FKBP) are, like cyclophilins, proline isomerases that bind to another immunosuppressive drug, FK506 and mediate its pharmacology. While there is no published evidence yet that FKBPs are involved in the pathogenesis of SARS-CoV-2, several groups have demonstrated that FKBP1A is important for assembly of the virus that caused the 2002-2004 SARS pandemic, SARS-CoV (Pfefferle, S., et al., 2011; PLOS Pathogens doi: 10.1371/journal.ppat.1002331). SARS-CoV is highly similar to SARS-CoV-2, and, in fact, both viruses use ACE2 as their cellular receptor. Therefore, it is reasonable to include FKBP1A in investigations of a potential role for FKBP1A in SARS-CoV-2 pathogenesis.

GeneCopoeia provides non-viral and lentiviral ORF cDNA clones in 250+ vector types for FKBP1A in human, mouse, and rat.

 

View all human FKBP1A ORF cDNA clones

Humans have two known isoforms of FKBP1A. You can order human FKBP1A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below

 

Product ID Accession Symbol Alias Description
R0062 NM_000801.4 FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Homo sapiens FK506 binding protein 1A (FKBP1A), transcript variant 1, mRNA.
Z8544 NM_001199786.1 FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Homo sapiens FK506 binding protein 1A (FKBP1A), transcript variant 3, mRNA.

 

View all mouse M. musculus FKBP1A ORF cDNA clones

Mouse M. musculus has four known isoforms of FKBP1A. You can order mouse FKBP1A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

 

Product ID Accession Symbol Alias Description
Mm02519 NM_008019.2 Fkbp1a 12kDa, FKBP12, FKBP12-T1, FKBP12-T2, Fkbp, Fkbp1, mFKBP1, mFKBP12 Mus musculus FK506 binding protein 1a (Fkbp1a), mRNA.
Mm38146 NM_001302078.1 Fkbp1a FKBP12, Fkbp, Fkbp1 Mus musculus FK506 binding protein 1a (Fkbp1a), transcript variant 4, mRNA.
Mm38147 NM_001302079.1 Fkbp1a FKBP12, Fkbp, Fkbp1 Mus musculus FK506 binding protein 1a (Fkbp1a), transcript variant 5, mRNA.
Mm38985 NM_001355078.1 Fkbp1a FKBP12, Fkbp, Fkbp1 Mus musculus FK506 binding protein 1a (Fkbp1a), transcript variant 8, mRNA.

 

View all rat R. norvegicus FKBP1A ORF cDNA clones

Rat R. norvegicus has only one known isoform of FKBP1A. You can order rat FKBP1A ORF clones in more than 250 non-viral and lentiviral vector types by clicking on the Product ID for each isoform in the table below.

 

Product ID Accession Symbol Alias Description
Rn24112 NM_013102.3 Fkbp1a FKBP12, Fkbp2 Rattus norvegicus FKBP prolyl isomerase 1A (Fkbp1a), mRNA.

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

SARS-CoV-2 human interacting protein ORF cDNA plasmid clones

A recent publication in Nature (Gordon, et al., 2020) described the results of an affinity purification-mass spectrometry (AP-MS) screen that identified 332 human proteins that physically interact with 26 SARS-CoV-2 viral proteins. While it is unlikely that all 332 interactions are physiologically relevant, Gordon, et al. demonstrated that 66 of these human host proteins are potential targets of known drugs used for other purposes, and that a subset of these drugs inhibit viral replication in vitro.

You can order all the clones encoding these human interacting proteins, either individually or as a complete library, in any of our 250+ non-viral or lentiviral vector types. To order complete libraries, please visit our ORF cDNA library page.