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AAVPrime™ Adeno-associated virus Serotype Testing Kit

Buy 3 GFP control particles for $399*

GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (Cat. No. AA320) contains 9 premade GFP-expressing AAV in serotypes 1, 2, 3, 6, 7, 8, 9, DJ and DJ/8.

AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit Plus (Cat. No. AA321) contains 12 premade GFP-expressing AAV in serotypes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, DJ and DJ/8. All AAV particles are offered at 5 x 1012 GC/ml, in 25 µl stocks, except that serotype 4 and 10 are offered at 5 x1011-1012 GC/ml. These kits are ideal for testing the infection efficiency of AAV serotypes on different cell types.

 


AAV of different serotypes carrying GFP were used to transduce the HT1080 cell line at MOI 20,000 as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.

 

Why do different AAV serotypes show tissue tropism?

All AAV serotypes are non-enveloped, single-stranded DNA parvoviruses. They share some properties including genome size, organization, and similar inverted terminal repeats. Despite similarities, different serotypes display significant differences in transduction efficiency and tissue tropism. AAV cellular entry is determined by its interactions with specific cell surface glycans and proteinaceous receptor(s). Different AAV serotypes have evolved distinctive interactions with the same receptor, therefore displaying various tissue tropism.

Which serotype should I use in my experiment?

Serotype Primary Target Tissues Description
AAV-1 Muscle Best for cardiac muscle, skeletal muscle, neuronal and glial tissue.
AAV-2 Muscle, Liver, Retina Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain.
AAV-3 Megakaryocytes Best for megakaryocytes, muscle, liver, lung, and retina.
AAV-4 Retina Best for neurons, muscle, brain, and retina.
AAV-5 Lung Best for lung, neurons, synovial joint, retina, and pancreas.
AAV-6 Muscle, Lung Best for lung, liver, and heart.
AAV-7 Muscle, Retina, Neurons Best for muscle, neurons, and liver.
AAV-8 Liver Best for muscle, brain, liver, and retina.
AAV-9 Various Best for muscle, heart, liver, lung, and brain.
AAV-10 Pleura, CNS Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver.
AAV-DJ Various A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro.
AAV-DJ/8 Various A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo.

AAV serotypes tropisms in tissue types

 

Buy Cat # Product Description Titer Size     Price

NEW!AA321

AAVPrime™ AAV Serotype Testing Kit Plus

5 x 1011 – 5 x 1012 GC/ml.  

12 x 25 µl    $1395
AA320 AAVPrime™ AAV Serotype Testing Kit 5 x 1012 GC/ml 9 x 25 µl

$795

 

NEW! AAV of different serotypes expressing GFP can be ordered separately as AAV control particles.

AAV of different serotypes expressing GFP
AA601

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 1.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350
AA001

eGFP AAVPrime™ Purified Adeno-associated Viral Particles      
Serotype: 2.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350
AA801

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 3.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350
AB001

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 4.   Promoter: CMV.

≥ 5×1011 GC/ml

25 µl $350

AB201

eGFP AAVPrime™ Purified Adeno-associated Viral Particles 
Serotype: 5.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

AB401

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 6.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

AB601

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 7.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

AB801

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 8.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

AC001

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 9.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

AC201

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: 10.   Promoter: CMV.

≥ 5×1011 GC/ml

25 µl $350

AA201

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: DJ.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

AA401

eGFP AAVPrime™ Purified Adeno-associated Viral Particles
Serotype: DJ/8.   Promoter: CMV.

≥ 5×1012 GC/ml 25 µl $350

 

* Must purchase 25ul premade AAV GFP control particles in increments of 3 for discounted price. Purchasing fewer than 3 will not be eligible for the discount. Not valid on 100 ul AAV. Not valid on custom AAV. Offer ends Nov 30, 2019.