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COVID-19 Coronavirus Lentiviral and AAV Solutions

General information

GeneCopoeia carries complete suites of state-of-the-art products for gene delivery using lentivirus and adenoassociated virus (AAV).

GeneCopoeia's lentiviral solutions include:

  • Lentiviral plasmids. Available for expressing ORF cDNAs, gene promoters, miRNA precursors and inhibitors, CRISPR sgRNAs, and shRNAs for do-it-yourself, 3rd generation lentiviral packaging. Use the "Plasmids" tab for ordering information.
  • Ready-to-use particles. For infection of most mammalian cell types with either standard VSV-G pseudotyping, or for infection of ACE2-expressing cells with SARS-CoV-2 Spike glycoprotein pseudotyping. These particles can be produced in either in vitro or in vivo grade. See the lentivirus drop down menus below for ordering information.
  • Packaging kits. Enable you to package lentiviral transfer plasmids with either standard VSV-G pseudotyping or SARS-CoV-2 Spike glycoprotein pseudotyping. Use the packaging kits drop down menu below for ordering information.

GeneCopoeia's AAV solutions include:

  • Ready-to-use particles. For infection of many mammalian cell types in nearly all available serotypes. These particles are be produced in in vivo grade, and can be used either in vitro or in vivo. Use the AAV drop down menu below for ordering information.
  • Serotype testing kits. For determining which AAV serotype(s) are best for infecting your cells of interest.

 

Lentivirus expressing SARS-CoV-2 viral proteins

Lentiviral particles for SARS-CoV-2 viral protein expression are available for 2 primary applications:

  1. Pseudotyping of lentivirus with the SARS-CoV-2 Spike (S) protein. Lentiviruses in common usage today are produced with the Vesicular Stomatis Virus G glycoprotein (VSV-G) expressed in the viral envelope, which enables the lentivirus to enter a wide range of mammalian cell types. However, during lentivirus packaging, the packaging plasmid encoding VSV-G can be replaced with a plasmid expressing SARS-CoV-2 Spike glycoprotein, which more narrowly limits their cell type infectivity to cells expressing ACE2, the human receptor for SARS-Cov-2 infection (Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052). These particles can be used for several applications, including vaccine development and testing antibodies that block cellular entry by SARS-CoV-2. If you want to order lentiviral particles pseudotyped with SARS-CoV-2 Spike protein, please contact inquiry@genecopoeia.com.
  2. Expression of SARS-CoV-2 viral proteins from standard VSV-G pseudotyped lentivirus in stable cell lines.To order high titer, ready-to-use lentiviral particles expressing SARS-CoV-2 viral proteins in one of our more than 80 lentiviral vector types, select the product ID from the table below.
Product ID Protein accession Symbol Alias Description
NV202 YP_009725297.1 orf1ab GU280_gp01 leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV203 YP_009725298.1 orf1ab GU280_gp01 nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV204 YP_009725299.1 orf1ab GU280_gp01 nsp3n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV205 YP_009725300.1 orf1ab GU280_gp01 nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV206 YP_009725301.1 orf1ab GU280_gp01 3C-like proteinase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV207 YP_009725302.1 orf1ab GU280_gp01 nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV208 YP_009725303.1 orf1ab GU280_gp01 nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV209 YP_009725304.1 orf1ab GU280_gp01 nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV210 YP_009725305.1 orf1ab GU280_gp01 nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV211 YP_009725306.1 orf1ab GU280_gp01 nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV212 YP_009725307.1 orf1ab GU280_gp01 RNA-dependent RNA polymerase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV213 YP_009725308.1 orf1ab GU280_gp01 helicase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV214 YP_009725309.1 orf1ab GU280_gp01 3'-to-5' exonuclease [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV215 YP_009725310.1 orf1ab GU280_gp01 endoRNAse [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV216 YP_009725311.1 orf1ab GU280_gp01 2'-O-ribose methyltransferase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV218 YP_009725312.1 orf1a GU280_gp01 produced by pp1a only [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV219 YP_009724390.1 S GU280_gp02 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene (codon optimized)
NV220 YP_009724390.1 S1 GU280_gp02 surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide (codon optimized)
NV221 YP_009724390.1 S2 GU280_gp02 surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)
NV222 YP_009724390.1 RBD GU280_gp02 Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)
NV223 YP_009724391.1 ORF3a GU280_gp03 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV224 YP_009724392.1 E GU280_gp04 envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV225 YP_009724393.1 M GU280_gp05 membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV226 YP_009724394.1 ORF6 GU280_gp06 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV227 YP_009724395.1 ORF7a GU280_gp07 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV228 YP_009725318.1 ORF7b

GU280_gp08

 ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV229 YP_009724396.1 ORF8 GU280_gp09 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV230 YP_009724397.2 N GU280_gp10 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV231 YP_009725255.1 ORF10 GU280_gp11 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene

 

*Note: Mature peptides are produced as a result of proteolytic processing of larger precursor proteins. To enable expression of these proteins in cells, an ATG has been added to the 5' end of the ORF sequence.

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

 

Lentivirus expressing host proteins necessary for infection by SARS-CoV-2

 

Lentivirus particles expressing ACE2 

  • ACE2 ORF-expressing lentiviral particles, expressed in your choice of more than 80 vector types, are available for human, mouse, and rat.

 

Lentivirus particles expressing TMPRSS2 

  • TMPRSS2 ORF-expressing lentiviral particles, expressed in your choice of more than 80 vector types, are available for human, mouse, and rat.

 

Premade lentivirus expressing human host factors needed for SARS-CoV-2 pathogenesis 

 

Buy Catalog Volume Titer Promoter Selection marker Tag Vector name Price
Premade lentivirus expressing human ACE2
LPP-U1285-Lv152-050-S 50 ul >108 TU/mL
Purified		 CMV Hygromycin Untagged pReceiver-Lv152 $995
LPP-U1285-Lv152-100-S 100 ul >108 TU/mL
Purified		 CMV Hygromycin Untagged pReceiver-Lv152 $1495
LPP-U1285-Lv152-200-S 100 ul >108 TU/mL
Purified		 CMV Hygromycin Untagged pReceiver-Lv152 $1995
LPP-U1285-Lv152-400-S 400 ul >108 TU/mL
Purified		 CMV Hygromycin Untagged pReceiver-Lv152 $2995
LPP-U1285-Lv152-A00-S 1000 ul >108 TU/mL
Purified		 CMV Hygromycin Untagged pReceiver-Lv152 $4995

 

  Warranty statement and limited use license   MSDS
 

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

 

Adenoassociated virus (AAV)

Order AAV particles expressing SARS-CoV-2 viral proteins

To order AAV particles expressing SARS-CoV-2 viral proteins in one of out more than 250 non-viral and lentiviral vector types, select the product ID from the table below and contact inquiry@genecopoeia.com.

Product ID Protein accession Symbol Alias Description
NV202 YP_009725297.1 orf1ab GU280_gp01 leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV203 YP_009725298.1 orf1ab GU280_gp01 nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV205 YP_009725300.1 orf1ab GU280_gp01 nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV206 YP_009725301.1 orf1ab GU280_gp01 3C-like proteinase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV207 YP_009725302.1 orf1ab GU280_gp01 nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV208 YP_009725303.1 orf1ab GU280_gp01 nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV209 YP_009725304.1 orf1ab GU280_gp01 nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV210 YP_009725305.1 orf1ab GU280_gp01 nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV211 YP_009725306.1 orf1ab GU280_gp01 nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV212 YP_009725307.1 orf1ab GU280_gp01 RNA-dependent RNA polymerase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV213 YP_009725308.1 orf1ab GU280_gp01 helicase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV214 YP_009725309.1 orf1ab GU280_gp01 3'-to-5' exonuclease [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV215 YP_009725310.1 orf1ab GU280_gp01 endoRNAse [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV216 YP_009725311.1 orf1ab GU280_gp01 2'-O-ribose methyltransferase [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV218 YP_009725312.1 orf1a GU280_gp01 produced by pp1a only [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV219 YP_009724390.1 S GU280_gp02 surface glycoprotein;structural protein; spike protein [Severe acute respiratory syndrome coronavirus 2], full-length gene (codon optimized)
NV220 YP_009724390.1 S1 GU280_gp02 surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide (codon optimized)
NV221 YP_009724390.1 S2 GU280_gp02 surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)
NV222 YP_009724390.1 RBD GU280_gp02 Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide* (codon optimized)
NV223 YP_009724391.1 ORF3a GU280_gp03 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV224 YP_009724392.1 E GU280_gp04 envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV225 YP_009724393.1 M GU280_gp05 membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV226 YP_009724394.1 ORF6 GU280_gp06 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV227 YP_009724395.1 ORF7a GU280_gp07 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV228 YP_009725318.1 ORF7b

GU280_gp08

 ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV229 YP_009724396.1 ORF8 GU280_gp09 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV230 YP_009724397.2 N GU280_gp10 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV231 YP_009725255.1 ORF10 GU280_gp11 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene

 

*Note: Mature peptides are produced as a result of proteolytic processing of larger precursor proteins. To enable expression of these proteins in cells, an ATG has been added to the 5' end of the ORF sequence.

For more information or to request a quote, please contact inquiry@genecopoeia.com.

 

Custom AAV expressing ACE2

  • ACE2 ORF-expressing AAV particles, available with a choice of pomoters and serotypes, ara available for human, mouse, and rat.

 

Custom AAV expressing TMPRSS2

  • TMPRSS2 ORF-expressing AAV particles, available with a choice of pomoters and serotypes, ara available for human, mouse, and rat.

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.