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GeneHero™ CRISPRa Stable Cell Lines

GeneHero™ CRISPRa Stable Cell Lines are mammalian cell lines stably expressing synergistic activation mediators (SAM) for the study of transcriptional activation of endogenous genes. These CRISPRa cell lines have stably integrated human codon-optimized dCas9 fused at the C-terminus of transcriptional activator VP64, and activation helper protein complex MPH (MS2-p65-HSF1).

The cell lines are monoclonal with verified dCas9-VP64/MPH genome integration and expression. Simply introduce promoter-targeting sgRNA or CRISPRa sgRNA libraries into the CRISPRa cell lines. They are ideal for the study of gene expression activation and genetic screening.

SAM Transcription activation by CRISPRa cell line expressing dCas9-VP64 and MPH

Verification of SAM Transcription Activation in GeneHero™ CRISPRa Cell Lines

CRISPR transcription activation in cell line SL302

As shown in the figures above, the transcription activation of VEGFA (A), EGFR (B) and HBG (C) was measured by qPCR in the HEK293 cell line expressing dCas9-VP64 and MPH complex (Cat# SL302) with or without transcription activation. The amplification curves of CRISPRa cell lines transduced with sgRNAs are shown in green, those without sgRNA transduction are shown in blue. The transcription levels are normalized to those of GAPDH for comparison. The transcription fold changes of genes are shown (D) by comparison of cell line transduced with and without sgRNA.

 

 

Certificate of Analysis: GeneHero™ CRISPRa Stable Cell Line     Available upon request.

 

Buy Catalog# Cell Line SAM Selection Marker Description Price, Academic Price, Industry
SL302 HEK293 dCas9-VP64/MPH Blasticidin
Hygromycin
HEK293 stably expressing dCas9-VP64 and MPH complex $945 $2695
SL304 MCF7 dCas9-VP64/MPH Blasticidin
Hygromycin
MCF7 stably expressing dCas9-VP64 and MPH complex $1395 $3995
We also offer services for:
  • Custom CRISPRa Cell Lines
  • CRISPRa sgRNA

Contact us at inquiry@genecopoeia.com, or call 866-360-9531 or 301-762-0888 for a quote.