GeneHero™ CRISPRa Stable Cell Lines are mammalian cell lines stably expressing synergistic activation mediators (SAM) for the study of transcriptional activation of endogenous genes. These CRISPRa cell lines have stably integrated human codon-optimized dCas9 fused at the C-terminal of transcriptional activator VP64, and activation helper protein complex MPH (MS2-p65-HSF1).
The cell lines are monoclonal with verified dCas9-VP64/MPH genome integration and expression. Simply introduce promoter-targeting sgRNA or CRISPRa sgRNA libraries into the CRISPRa cell lines. They are ideal for the study of gene expression activation and genetic screening.
Verification of SAM Transcription Activation in GeneHero™ CRISPRa Cell Lines
As shown in the figures above, the transcription activation of VEGFA (A), EGFR (B) and HBG (C) was measured by qPCR in the HEK293 cell line expressing dCas9-VP64 and MPH complex (Cat# SL302) with or without transcription activation. The amplification curves of CRISPRa cell lines transduced with sgRNAs are shown in green, those without sgRNA transduction are shown in blue. The transcription levels are normalized to those of GAPDH for comparison. The transcription fold changes of genes are shown (D) by comparison of cell line transduced with and without sgRNA.
- Custom CRISPRa Cell Lines
- CRISPRa sgRNA
