GeneCopoeia's IndelCheck™ CRISPR insertion or deletion (indel) detection system provides a powerful means for assisting you in your genome editing applications. The IndelCheck™ system can be used for:
- CRISPR sgRNA functional validation (Figure 1) before undertaking long genome editing projects (3-6 months for genome edited cell lines, or 6-9 months for genome-edited mouse lines), saving you a great deal of time and effort by eliminating CRISPR sgRNAs with poor cleavage efficiency.
- Screening cell clones for knockout (KO) and knock-in (KI) modifications (Figure 2).
Advantages
- Complete system to simplify your CRISPR validation and edited clone screening
- Robust amplification for the target site PCR. No genomic DNA isolation is required
- Easy to use T7 endonuclease I assay with optimized conditions and positive control
Order Information
Order Information
The IndelCheck™ system consists of the following major components:
- Target site PCR kit V2.0. For amplification of targeted genomic regions from cell lysates without genomic DNA isolation. Now in Version 2.0, the Target Site PCR kit is an contains buffer, SuperHeRo™ DNA polymerase, and nucleotides together in one mix for greater convenience.
- T7 endonuclease I assay kit. For detection of CRISPR-introduced indel mutations near target site(s).
- Smart-Join™ Blunt-end PCR Cloning Kit. For cloning of PCR products to be used for sequencing and identification of CRISPR-mediated genomic modifications.