ExoCt™ RT-qPCR System

ExoCt™ First-Strand cDNA Synthesis Kit
ExoCt™ First-Strand cDNA Synthesis Kit
ExoCt™ First-Strand cDNA Synthesis Kit
ExoCt™ First-Strand cDNA Synthesis Kit
ExoCt™ SYBR® Green RT-qPCR Kit
ExoCt™ SYBR® Green RT-qPCR Kit
ExoCt™ SYBR® Green RT-qPCR Kit
ExoCt™ SYBR® Green RT-qPCR Kit

ExoCt™ First-Strand cDNA Synthesis Kit ExoCt™ First-Strand cDNA Synthesis Kit ExoCt™ SYBR® Green RT-qPCR Kit ExoCt™ SYBR® Green RT-qPCR Kit

Price: $280.00 $800.00 $458.00 $1,230.00
Catalog#:
  • QP125
  • QP126
  • QP131
  • QP132
Size: 20 rxn 60 rxn 20 RT and 200 qPCR 60 RT and 600qPCR
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Product Information

GeneCopoeia’s ExoCt™ RT-qPCR System allows you to analyze gene expression by real-time qPCR directly from exosomes without any RNA purification. The ExoCt™ First-Strand cDNA Synthesis Kit contains ExoCt™ RT-for-All™ Buffer and ExoCt™ PAP/RTase Mix, which were specifically developed for robust first-strand cDNA synthesis covering a variety of RNA templates, including miRNA, mRNA, LncRNA, etc., from exosome lysates in a single reaction. The ExoCt™ SYBR® Green RT-qPCR Kit includes both RT and qPCR reagents, which combines PCR technology and SYBR® Green to make fast and accurate quantification of exosome RNAs. The system includes the ExoCt™ First-Strand cDNA Synthesis Kit and the ExoCt™ SYBR® Green RT-qPCR Kit.

Advantages

  • Direct detection of gene expression from exosomes without any RNA purification
  • Convenient reverse transcription of all exosomal miRNA, mRNA, and LncRNA in one reaction simultaneously
  • Flexible analysis and quantitation of different kinds of RNA in downstream qPCR reactions

Simple Workflow

Isolated exosomes are lysed by adding ExoCt™ Lysis Buffer, then heating at 37°C for 10 min and 75°C for 10 min. Exosome lysates are used directly for ExoCt™ RT reactions, and the cDNA products can be used for qPCR to detect miRNA, mRNA, and LncRNA, etc.