Introduction
GeneCopoeia's Luc-Pair™ Renilla Luciferase Assay Kit is the perfect choice for high sensitivity detection of Renilla luciferase (RLuc) activity. The Luc-Pair™ Renilla Luciferase HS Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) using micro-plate or single-tube luminometers, and is compatible with GeneCopoeia, Promega and many other Rluc vectors.
Advantages
- High sensitivity. Renilla luciferase signals exhibit greatest sensitivity (Figure 1).
- Versatility. Designed for assays with many different eukaryotic (vertebrates, lower invertebrates) cells using micro-plates or single-tube luminescence reader.
- Low background. Low background luminescence. No subtraction is required from readings.
- Reproducibility. Reliable, linear results for a concentration range over several orders of magnitude.
GeneCopoeia also offers the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (for enhanced stability) and Luc-Pair™ Duo-Luciferase HS Assay Kit (for high sensitivity) for applications requiring luciferase readings.
Figure 1. Activity of Renilla luciferase signals using GeneCopoeia Luc-Pair™ Renilla Luciferase HS Assay Kit. HEK 293 cells were transfected with Promega pGL4.75 reporter vector for 48 hours. RLuc (RLuc-GeneCopoeia) activity was measured as described in the procedure. Promega’s Luciferase Assay System was used (RLuc-Promega) in comparison.
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2022
Frequently Asked Questions: Luciferase Assays
Answer: “RLU” stands for “Relative Light Unit”. Luminometers do not measure light output, in terms of numbers of photons, directly.
Answer: All GeneCopoeia luciferase kits are compatible with all known luminometers.
Answer: All GeneCopoeia luciferase reagents are stable for at least 6 months if stored at -20°C.
Answer: To prevent loss of activity, it is best to avoid repeated freezing and thawing. We recommend pipetting small aliquots into new, clean, sterile tubes and placing those aliquots at -20°C. Once you have thawed one of the aliquots, discard it instead of putting it back in the freezer.
Answer: The Gaussia luciferase substrate, coelenterazine, is also the substrate for Renilla luciferase, so it is compatible with Renilla. However, coelenterazine cannot be used with Firefly (Photinus pyralis) luciferase, which instead uses luciferin as its substrate.
Answer: Unfortunately, the individual components of GeneCopoeia's luciferase kits cannot be purchased separately at this time.
Answer: Typically, between 12 and 24 hours.
Answer: The
Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (For Enhanced Stability),
Luc-Pair™ Duo-Luciferase Assay Kit HT (For High Throughput),
Luc-Pair™ Firefly Luciferase HT Assay Kit, and
Luc-Pair™ Renilla Luciferase HT Assay Kit (For High Throuhput), which provide greater signal stability, are most useful when assaying a relatively large number of samples. Their enhanced signal stability means that there will be little sample-to-sample variation due to signal decay. Conversely, the
Luc-Pair™ Duo-Luciferase HS Assay Kit (For High Sensitivity),
Luc-Pair™ Firefly Luciferase HS Assay Kit (For High Sensitivity), and
Luc-Pair™ Renilla Luciferase HS Assay Kit (For High Sensitivity) are used when maximal signal intensity is required, but the trade-off is that the signal decays more rapidly under typical laboratory conditions. These kits are not appropriate for measuring the activities of large numbers of samples, unless sample injectors are used.
