PPAR transcriptional response element (TRE) Clone

GeneCopoeia's GLuc-ON™ PPAR transcriptional response element (TRE) clone enables analysis of transcriptional activity of peroxisome proliferator-activated receptors (PPARs).  PPARs are a family of three nucleus-localized transcription factors-PPARα, PPARβ/δ, and PPARγ that lead to peroxisome proliferation in response to lipid ligands. PPARs are important players in treatments of various diseases, such as obesity and diabetes.

The GLuc-ON™ PPAR TRE clone contains tandem repeats of PPAR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The PPAR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PPAR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.

 
Buy Cat.No. Product name Price
TR112 PPAR TRE clone for peroxisome proliferator-activated receptor signaling pathway $350
SEAP-PA01 Secreted alkaline phosphatase (SEAP) clone for normalization $395
TR001 TRE negative control clone $300
GAPDH-PG02 GAPDH positive control clone $375

 

 

Secrete-Pair Luminescence Assay Kits

Buy Catalog# Product Description Price
LF031 Secrete-Pair Dual Luminescence Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF032 Secrete-Pair Dual Luminescence Assay Kit (300 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF033 Secrete-Pair Dual Luminescence Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF061 Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) inquire
LF062 Secrete-Pair Gaussia Luciferase Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) inquire

 

EndoFectin transfection reagents

Buy Cat.No. Product name Price Data sheets
EF013 EndoFectin™ Max transfection reagent (1 mL) $295 Download
EF014 EndoFectin™ Max transfection reagent (3 mL) $589
EF001 EndoFectin™ Lenti transfection reagent (1 mL) $245 Download
EF002 EndoFectin™ Lenti transfection reagent (3 mL) $489
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $355 Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $705
EF007 EndoFectin™ Expi293 transfection reagent (1 mL) $320  
EF008 EndoFectin™ Expi293 transfection reagent (3 mL) $640  

 

Validation Data

Figure 1. Activation of the GLuc-ON™ PPAR TRE by exogenous expression of PPAR. 293T cells were were co-transfected in the presence of 1 µM rosiglitazone with (left to right) negative TRE clone + negative expression clone, negative TRE clone + PPAR expression clone, PPAR TRE clone + negative expression clone, or PPAR TRE clone + PPAR expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.