TCF/LEF transcriptional response element (TRE) lentiviral clone

GeneCopoeia provides the GLuc-ON™ TCF/LEF transcriptional response element (TRE) lentiviral clone for analysis of the effects on Wnt signaling pathways mediated by TCF/LEF transcription factors, which recruit β-catenin to activate the promoters of several genes critical for function of the Wnt pathway. Wnt signaling plays fundamental roles in embryonic development, and its misregulation has been implicated in diseases such as cancer and type 2 diabetes.

The GLuc-ON™ TCF/LEF TRE lentiviral clone contains tandem repeats of TCF/LEF upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The TCF/LEF response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ TCF/LEF TRE lentiviral clone is provided as transfection-ready DNA. A negative (non-activating) control lentiviral clone is also available for purchase.

 
Buy Cat.No. Product name Price
TR202 TCF/LEF TRE lentiviral clone for Wnt signaling pathway $450
TR002 TRE negative control clone $400

 

 

Secrete-Pair Luminescence Assay Kits

Buy Catalog# Product Description Price
LF031 Secrete-Pair Dual Luminescence Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF032 Secrete-Pair Dual Luminescence Assay Kit (300 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF033 Secrete-Pair Dual Luminescence Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF061 Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) inquire
LF062 Secrete-Pair Gaussia Luciferase Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) inquire

 

 

EndoFectin transfection reagents

Buy Cat.No. Product name Price Data sheets
EFM1004-01 EndoFectin™ Max transfection reagent (1 mL) $295 Download
EFM1004-02 EndoFectin™ Max transfection reagent (3 mL) $589
EFL1001-01 EndoFectin™ Lenti transfection reagent (1 mL) $245 Download
EFL1001-02 EndoFectin™ Lenti transfection reagent (3 mL) $489
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $355 Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $705
EF007 EndoFectin™ Expi293 transfection reagent (1 mL) $320  
EF008 EndoFectin™ Expi293 transfection reagent (3 mL) $640  

 

 

 

Lenti-Pac™ Lentiviral packaging systems

 
Buy Catalog# Product Description Manual or datasheets Price
LT001 Lenti-Pac HIV Expression Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent /wp-content/uploads/2017/11/Lenti-Pac-HIV-Expression-Packaging-Kit-Manual-122818.pdf inquire
LT002 Lenti-Pac HIV Expression Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent /wp-content/uploads/2017/11/Lenti-Pac-HIV-Expression-Packaging-Kit-Manual-122818.pdf inquire
LT005 Lenti-Pac HIV qRT-PCR Titration Kit (20 RT reactions, 50 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. /wp-content/uploads/2017/06/Lenti-Pac_qRT-PCR_Titration_Kit_manual.pdf inquire
LT006 Lenti-Pac HIV qRT-PCR Titration Kit (40 RT reactions, 100 PCR reactions) qRT-PCR based lentiviral titration to determine the copy numbers of HIV lentiviral particles. /wp-content/uploads/2017/06/Lenti-Pac_qRT-PCR_Titration_Kit_manual.pdf inquire
LT007 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles /wp-content/uploads/2017/06/Lenti-Pac_Lentivirus_Concentration_Solution_Manual.pdf inquire
LT008 Lenti-Pac 293Ta Cell Line (1.5 x 106 cells) 293Ta Lentiviral packaging cell line /wp-content/uploads/2017/06/293ta_packaging_cell_line.pdf inquire
MP004 Mycoplasma PCR detection kit (50 rxns) Quick detection of mycoplasma contamination in cell culture or virus Mycoplasma PCR Detection Kit inquire
 

 

 

Lenti-Pac™ Lentiviral concentration solution

 

Buy Catalog# Product Description Price
LT007 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles inquire

 

 

 

 

Validation Data

Figure 1. Activation of the GLuc-ON™ Wnt TRE by lithium chloride. HEK293a cells were transduced with  negative control lentiviral partices or the GLuc-ON™ TCF/LEF TRE lentiviral particles. Transduced cells were incubated for 48 hours, followed by 6 days incubation in the presence of puromycin. Puromycin-resistant cells were then treated with lithium chloride for 18 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.