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COVID-19 Coronavirus CRISPR and shRNA Solutions

General information

GeneCopoeia provides CRISPR-Cas9 solutions for gene knockout, knock-in, mutagenesis, tagging and more. Cas9 and sgRNA are available as either transfection-ready plasmid DNA, ready-to-use lentiviral and AAV particles, or as components for assembling ribonucleoprotein (RNP) complexes.

In addition, we provide a collection of 7 lung cancer cell lines with the Cas9 nuclease stably integrated, which are ideal for lentiviral CRISPR or CRISPR knockout library screens, and one lung cancer cell line for CRISPR-mediated gene activation, which carries the dCas9-VP64 and MPH complex.

CRISPR-Cas9 tools can be produced for modification of the genes expressing the proteins that the virus interacts with, including ACE2, the receptor for SARS-CoV-2 on respiratory epithelial cells, and TMPRSS2, a serine protease required for S protein priming.

CRISPR-Cas9 solutions for modification of host genes
Of the multiple host proteins required for SARS-CoV-2 pathogenesis, two of the most important are ACE2, the receptor that the viral spike protein uses for cell entry, and TMPRSS2, a serine protease required for S protein priming. You can purchase ready-to-use plasmid clones, lentiviral particles, or AAV particles that can be used for CRISPR-mediated modification of the ACE2 and TMPRSS2 using the links below. These products are part of GeneCopoeia’s GeneHero™ CRISPR-Cas9 solutions.

GeneHero™ plasmid clones for modification of human host genes required for SARS-CoV-2 pathogenesis

 

GeneHero™ lentiviral particles for modification of human host genes required for SARS-CoV-2 pathogenesis

 
  • ACE2 CRISPR-Cas9 lentiviral particles for human, mouse, and rat.
  • TMPRSS2 CRISPR-Cas9 lentiviral particles for human, mouse, and rat.

GeneHero™ AAV particles for modification of human host genes required for SARS-CoV-2 pathogenesis

    For more information or to request a quote, please contact inquiry@genecopoeia.com.  
Premade CRISPR-Cas9 stable human lung cancer cell lines
The cell lines below carry the S. pyogenes Cas9 nuclease stably integrated into either the human AAVS1 “Safe Harbor” locus or at random sites resulting from lentivirus-mediated integration. These cell lines are part of GeneCopoeia’s collection of premade CRISPR-Cas9 stable cell lines. To obtain the datasheet for each cell line, please click on the designated catalog number.
Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
Human cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL501 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from AAVS1 site Lung cancer Puro $1395 $3995
SL533 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL504 A549 Human cell line A549 stably expressing Cas9 from AAVS1 site Lung cancer Hygro $1395 $3995
SL529 H1975 Human cell line H1975 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL536 NCI-H1437 Human cell line NCI-H1437 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL561 NCI-H661 Human cell line NCI-H661 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL578 HCC827 Human cell line HCC827 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
  For more information or to request a quote, please contact inquiry@genecopoeia.com.
CRISPR activation stable human lung cancer cell line
This cell line carries the dCas9-VP64 and MPH complex used for sgRNA-mediated gene transcriptional activation in the absence of double strand breaks (DSBs). This product is part of GeneCopoeia’s collection of premade CRISPRa (CRISPR activation) stable cell lines. To obtain the datasheet for each cell line, please click on the designated catalog number.
Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
SL306 A549 A549 stably expressing dCas9-VP64 and MPH complex, 2×106 cells Lung cancer Blasticidin Hygromycin $1395 $3995
 
Warranty statement and limited use license MSDS
  For more information or to request a quote, please contact inquiry@genecopoeia.com.  
shRNA

GeneCopoeia provides shRNA solutions for gene knockdown. shRNA tools are available as either transfection-ready plasmid DNA or ready-to-use lentiviral and AAV particle. shRNA tools can be produced for knockdown of the genes expressing the proteins that the virus interacts with, including ACE2, the receptor for SARS-CoV-2 on respiratory epithelial cells, and TMPRSS2, a serine protease required for S protein priming.

    For more information or to request a quote, please contact inquiry@genecopoeia.com.