Product Information
Microbiome and Bacterial infections are potential pathogenic risk for cancer and autoimmune diseases. GeneCopoeia’s 14-plex Bacterial Surface Antigen Array is a protein microarray enabling simultaneous detection of antibodies targeting to the surface antigens of 14 potential pathogenic bacterial strains in human. The array carries superior-quality purified bacterial surface antigens from Neisseria mucosa, Eubacterium yurii, Actinomyces oris, Rothia dentocariosa, Neisseria macacae, Neisseria sicca, Neisseria elongate, Neisseria flavescens, Aggregatibacter aphrophilus, Streptococcus parasanguinis, Lactobacillus gasseri, Streptococcus sanguinis, Blantia Coccoides, and H. pylori, spotted onto nitrocellulose membrane-coated microchips, which keep the conformational structure of the antigens. The antigens are spotted on chip with 5 different dilutions enabling optimal antigen-antibody binding with highest sensitivity for antibody detection. Using different fluorescent labeled 2nd antibodies make it feasible to detect multiple types of antibodies (IgG, IgM, IgA, IgE) from various biological specimens.
In addition to this premade array, arrays containing customized sets of antigens are available, as well as array profiling services and data analysis. To order premade or custom arrays, please contact us.
GeneCopoeia’s OmicsArray™ Interferon, Infection and Autoimmune 64-plex antigen array is part of the GeneCopoeia OmicsArray™ Antigen Microarray family.
References
1. Vogtmann E, Hua X, Yu G, et a. (2022) The Oral Microbiome and Lung Cancer Risk: An Analysis of 3 Prospective Cohort Studies. J Natl Cancer Inst. 114(11):1501-1510. doi: 10.1093/jnci/djac149
2. Di Pilato V, Freschi G, Ringressi MN, et al. (2016) The esophageal microbiota in health and disease. Ann N Y Acad Sci. 1381(1):21-33. doi: 10.1111/nyas.13127
3. Ramirez NA, Das A, Ton-That H. (2020) New Paradigms of Pilus Assembly Mechanisms in Gram-Positive Actinobacteria. Trends Microbiol. 28(12):999-1009. doi: 10.1016/j.tim.2020.05.008
4. Fang L, Chen J, Pan L, et al. (2023) Alterations of Gut Microbiome, Metabolome, and Lipidome in Takayasu Arteritis. Arthritis Rheumatol. 75(2):266-278. doi: 10.1002/art.42331.
Advantages of OmicsArray™ Antigen Microarrays
- Largest collection of pre-made whole-protein antigen microarrays on the market.
- Largest number of whole-protein antigens specifically focused on autoimmune diseae research.
- Best combination of number of antigens per array (up to 120) with number of samples that can be processed per slide (up to 15).
OmicsArray™ premade antigen arrays
For pricing, please inquire.
In addition to premade arrays, arrays containing customized sets of antigens are available, as well as array processing kits,
array profiling services and
data analysis.
Antigen microarray processing kits
Custom services
GeneCopoeia offers custom antigen microarray services in the following areas:
- Custom array printing. GeneCopoeia will create custom antigen microarrays built to your specifications.
- Sample processing. Send us your blood, plasma, tissue, or other biological sample and we will prepare it for processing and incubation with any of our premade antigen microarrays or custom-built antigen microarrays for autoantibody profiling and other applications. For information on sample types to submit, consult the FAQ
- Data analysis. Once samples are processed and incubated with an antigen microarray, we will analyze the raw data. The standard analysis service includes: 1) An Excel file of the Net Signal Intensity (NSI) for each antigen on the array, normalized to internal controls; and 2) a heat map
Additional analysis services, including proteomic analysis, pathway analysis, and more, are also available.
Technology overview
Technology overview
GeneCopoeia’s OmicsArray™ antigen microarrays contain up to 120 purified proteins spotted onto nitrocellulose filters, which are adhered to glass slides. In addition, 8 spots are included for normalization. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control. As little as 1 ul serum or 50 ul of other bio fluids are needed for each sample.
As shown in Figure 1, arrays are incubated with patient samples, and any antibodies in the samples bind to their cognate antigens on the array. The arrays are washed to remove unbound antibodies and other proteins, then co-incubated with Cy3- and Cy5-labeled secondary antibodies. The dual labeling strategy is intended to distinguish between immunoglobulin (Ig) subtypes present within samples. For example. a Cy3-labeled anti-IgG secondary antibody is used to detect IgG antibodies, and a Cy5-labeled anti-IgM secondary antibody is used to detect IgM antibodies. Fluorophore-labeled secondary antobodies are available for detecting IgA, IgD, IgE, IgG and IgM immunoglubulins, as well as IgG subclasses IgG1, IgG2, IgG3, and IgG4.
After washing to remove unbound secondary antibodies, signals are detected using a microarray scanner (e.g., GenePix® 4000B, InnoScan 710, or equivalents). The raw data is then be analyzed using GenePix® Pro 7.0, or Mapix software.
Figure 1. Workflow for detection of antibodies in samples using GeneCopoeia’s OmicsArray™ antigen microarrays.
FAQ
Frequently Asked Questions
Answer: You can send us virtually any biological sample, including whole blood, serum, plasma, interstitial fluid, semen, urine, and saliva. To learn more, please contact us at
inquiry@genecopoeia.com.
Answer: Yes.
Answer: Yes.
Answer: Yes.
Answer: Each nitrocellulose membrane is surrounded by a gasket to prevent leakage and cross-contamination.
Answer: a. Each slide carries 16 identical arrays. One of these is reserved for a PBS control, while the remaining 15 are used for incubation of each sample. Therefore, the total number of samples sent by the customer is preferably a multiple of 15. However, if you have fewer than 15 samples, we will need to charge you for the full cost of an entire slide of 15 samples. b. Theoretically, each of the different test groups requires a minimum of 3 samples to meet statistical reproducibility requirements. On this basis, the greater the number of samples, the higher the reproducibility of the results in the test group. In order to ensure the reliability of the results, the sample size of different test groups is recommended to be ≥3.
Answer: The antigen microarray uses fluorescence detection, and its sensitivity is higher and more stable than ELISA (colorimetry) and Western (chemiluminescence). The technical repetition correlation coefficient R2 between the arrays can reach 0.9 or more. The dynamic detection range is 1-65000. The larger the dynamic range, the more layers of signals that can be detected.
Answer: Please fully communicate with our Technical Support staff before the experiment to explain the purpose of the experiment and the sample status, to determine whether we can meet your needs and determine the experimental plan.
Answer: The antigens on the antigen array are all derived from self-antigens reported in the literature. For each of the different types of antigenic arrays, we can provide you with all the names of the antigens upon reques Please do not disclose this information to third parties.
Answer: Proteins are usually expressed and purified from E. coli or mammalian cells.
Answer: We usually spot the entire full-length protein in its native conformation. However, we can also spot truncated proteins or peptides if needed.
Answer: If you need a custom antigen microarray, we need to fully communicate with you about the following issues: a. The intellectual property of the antigen (whether from a published paper or a patent, etc.). We can only provide customized detection of antigens that do not involve patent protection; b. Basic information about the antigen (whether it is whole protein or peptide, molecular weight, domain, whether it is a membrane protein, etc.); c. Whether you can provide an antigen that meets the requirements of a custom array; d. If we need to provide the antigen to be tested, the corresponding cost and experimental time frame need to be accounted for separately.
Answer: The antigens on our predesigned arrays are usually human. However, for custom arrays, we can use antigens from virtually any organism.In addition, the arrays have been used with human, mouse, and rat samples.
Answer: Antigens are usually spotted at a concentration between 0.1 and 1.0 mg/ml, but each individual protein concentration requires optimization.
Answer: a. This difference may be related to the detection method. The protein used on the array is a non-denatured protein, which is different from western blot. The hybrid system of the chip is an antigen-trapping antibody, and the western blot antigen is slightly different from the antibody after interacting with the antigen; b. Please provide a graph of the results of western blot verification, so that we can better analyze the image and data of your verified indicators on the array.
