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GLuc-ON Transcriptional Response Assays

Introduction

GeneCopoeia's GLuc-ON™ transcriptional response element (TRE) clones enable rapid and sensitive signal pathway analysis in mammalian cell lines. Using a secreted Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ TRE  clones are designed for robust and sensitive activation of specific signaling pathways in response to environmental stimuli or other experimental manipulation. Each GLuc-ON™ TRE clone comes either in transfection-ready or lentiviral format, and contains tandem repeats of a TRE placed upstream of a minimal CMV promoter and the Gluc gene. 

GeneCopoeia is also now offering cell lines stably expressing Transcriptional Response Elements. Click on the TRE stable cell lines tab to order, or visit the Transcriptional Response Element Stable Cell Lines page to learn more.

Advantages

  • Live cell assays. Naturally secreted GLuc reporter makes lysing of cells unnecessary.
  • Real-time study. Data is generated quickly, closely resembling real-time activities
  • High-throughput compatible.
 

 

Applications

GLuc-ON™ TRE clones offer powerful tools for several applications, including:

  • Drug discovery or validation. Identify small molecules that either stimulate or inhibit a signaling pathway of interest.
  • Analyze response of cells to proteins and peptides.
  • Analyze response of cells to hormones.
  • Functional genomics. Analyze the biological effects on a signaling pathway in response to gene activation, overexpression, knockdown, knockout, or mutagenesis (Figure 1).
  • Viral infection. Analyze response of cells to viruses.

 

Figure 1. Application example: Functional genomics using TRE clones. HEK293a cells were co-transfected with Oct4 TRE clone + GFP expression clone, negative control clone + Oct4 expression clone, or Oct4 TRE clone + Oct4 expression clone. Transfected cells were incubated for 48 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation. 

 

How do GLuc-ON™ TRE clones work?

GLuc-ON™ TRE response elements are engineered to provide strong activation of GLuc expression with low background. The GLuc reporter is a secreted protein, permitting convenient and rapid detection in the cell culture medium without lysing the cells (Figure 2).

 

 

Figure 2. How GLuc-ON TRE clones work.