GeneCopoeia’s GLuc-ON™ Oct4 transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by octamer-binding transcription factor 4 (Oct4). Oct4 is a transcription factor necessary for maintaining embryonic stem cells in their undifferentiated state, and its misregulation is associated with cancer.
The GLuc-ON™ Oct4 TRE clone contains tandem repeats of an NFAT binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The Oct4 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ Oct4 TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the Oct4 GLuc-ON™ TRE by exogenous expression of Oct4. HEK293a cells were co-transfected with Oct4 TRE clone + GFP expression clone, negative control clone + Oct4 expression clone, or Oct4 TRE clone + Oct4 expression clone. Transfected cells were incubated for 48 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation.