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EndoFectin™ Transfection Reagents

Buy 1, Get 1 50% OFF EndoFectin Max; use promo code: BOGOMAX*

A versatile & efficient suite of robust transfection reagents

EndoFectin Max

Introducing EndoFectin™ Max

EndoFectin Max is a new lipid-based transfection reagent. This versatile transfection reagent has been tested and optimized for highly efficient transfection of a large collection of commonly used cell lines as well as many difficult-to-transfect cell lines.      

 

Advantages

  • Equal to or greater transfection efficiency compared with Lipofectamine® 3000 in plasmid transfection (Figure 1).
  • Superior transfection efficiency for a broad range of cell lines compared with Lipofectamine® 2000 (Figures 2 and 3).
  • Equal to or greater transfection efficiency compared with Lipofectamine® 2000 in siRNA transfection (Figure 4).
  • Low cytotoxicity (Figure 5).
  • Does not require removal of serum or culture medium, or removal of medium after transfection.
  • For overexpression, knockdown, knockout, as well as high-throughput applications

 

Buy Cat.No. Product name Price Protocol MSDS
EF013 EndoFectin™ Max transfection reagent (1 mL) $295 Download Download
EF014 EndoFectin™ Max transfection reagent (3 mL) $589

 

 

* Promotion is valid in the US & Canada only. Offer is only valid on purchases of EF014. Not valid on other EndoFectin transfection reagents. Promo code must be presented at the time of purchase. Not valid on previous purchases and cannot be combined with other discounts. Promotion ends August 31, 2021.

 

Experiment 1: Comparison of EndoFectin™ Max and Lipofectamine® 3000 in plasmid transfection

A B
       
C D
       
E F
 

Figure 1. Transfection of 6 commonly-used cell lines using either EndoFectin™ Max, Lipofectamine® 2000 (LP2000) or Lipofectamine® 3000 (LP3000). Cell lines were transfected with a firefly luciferase-expressing plasmid. Transfection efficiencies were assessed by luciferase activity (RLU). Note: for HEK293 (A), HeLa (C), A549 (D), H1299 (E), and 1321N1 (F), 0.15 uL/well of Lipofectamine® 3000 was used rather than 0.2 uL/well. Lipofectamine® is a trademark of Invitrogen.

 

Experiment 2: Comparison of EndoFectin™ Max and Lipofectamine® 2000 in plasmid transfection

A
 
B
 
C
 
Figure 2. Transfection of 17 commonly-used cell lines using either EndoFectin™ Max or Lipofectamine® 2000 (LP-2000). Cell lines with low transfection efficiency (A), medium transfection efficiency (B), and high transfection efficiency (C) were transfected with a firefly luciferase-expressing plasmid. Transfection efficiencies were assessed by luciferase activity (RLU). Lipofectamine® is a trademark of Invitrogen.

 

 
Figure 3. Assessment of EndoFectin™ Max transfection efficiencies using GFP visualization. 16 commonly-used cell lines were transfected with a GFP-expressing plasmid using either EndoFectin™ Max or Lipofectamine® 2000 (LP-2000). Cells were visualized 48 hours post-transfection under phase contrast or fluorescence microscopy (GFP filter). Lipofectamine® is a trademark of Invitrogen.
 

 

Experiment 3: Comparison of EndoFectin™ Max and Lipofectamine® 2000 in siRNA transfection

A B
 
Figure 4. Comparison of transfection of siRNA between EndoFectin™ Max and Lipofectamine® 2000, Cell lines H1299 (A) and HEK293 (B) were transfected with either a firefly luciferase-expressing plasmid (blue bars), or co-tranfected with a firefly luciferase-expressing plasmid and an anti-luciferase siRNA (red bars). Transfection efficiencies were assessed by luciferase activity (RLU). Lipofectamine® is a trademark of Invitrogen.

 

Experiment 4: Comparison of EndoFectin™ Max and Lipofectamine® 2000 for cytotoxicity

 
Figure 5. Comparison of cytotoxicity between EndoFectin™ Max and Lipofectamine® 2000. Lipofectamine® is a trademark of Invitrogen.

 

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