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EndoFectin Transfection Reagents

A versatile & efficient suite of robust transfection reagents

EndoFectin Max

Introducing EndoFectin™ Max

EndoFectin™ Max is a new lipid-based201 transfection reagent. This versatile transfection reagent has been tested and optimized for highly efficient transfection of a large collection of commonly used cell lines as well as many difficult-to-transfect cell lines.      

Advantages

  • Equal to or greater transfection efficiency compared with Lipofectamine® 3000 in plasmid transfection (Figure 1).
  • Superior transfection efficiency for a broad range of cell lines compared with Lipofectamine® 2000 (Figures 2 and 3).
  • Equal to or greater transfection efficiency compared with Lipofectamine® 2000 in siRNA transfection (Figure 4).
  • Low cytotoxicity (Figure 5).
  • Does not require removal of serum or culture medium, or removal of medium after transfection.
  • For overexpression, knockdown, knockout, as well as high-throughput applications

 

Buy Cat.No. Product name Price Protocol MSDS
EF013 EndoFectin™ Max transfection reagent (1 mL) $295 Download Download
EF014 EndoFectin™ Max transfection reagent (3 mL) $589
   

 

 

Experiment 1: Comparison of EndoFectin™ Max and Lipofectamine® 3000 in plasmid transfection

A B
       
C D
       
E F
 

Figure 1. Transfection of 6 commonly-used cell lines using either EndoFectin™ Max, Lipofectamine® 2000 (LP2000) or Lipofectamine® 3000 (LP3000). Cell lines were transfected with a firefly luciferase-expressing plasmid. Transfection efficiencies were assessed by luciferase activity (RLU). Note: for HEK293 (A), HeLa (C), A549 (D), H1299 (E), and 1321N1 (F), 0.15 uL/well of Lipofectamine® 3000 was used rather than 0.2 uL/well. Lipofectamine® is a trademark of Invitrogen.

 

Experiment 2: Comparison of EndoFectin™ Max and Lipofectamine® 2000 in plasmid transfection

A
 
B
 
C
 
Figure 2. Transfection of 17 commonly-used cell lines using either EndoFectin™ Max or Lipofectamine® 2000 (LP-2000). Cell lines with low transfection efficiency (A), medium transfection efficiency (B), and high transfection efficiency (C) were transfected with a firefly luciferase-expressing plasmid. Transfection efficiencies were assessed by luciferase activity (RLU). Lipofectamine® is a trademark of Invitrogen.

 

 
Figure 3. Assessment of EndoFectin™ Max transfection efficiencies using GFP visualization. 16 commonly-used cell lines were transfected with a GFP-expressing plasmid using either EndoFectin™ Max or Lipofectamine® 2000 (LP-2000). Cells were visualized 48 hours post-transfection under phase contrast or fluorescence microscopy (GFP filter). Lipofectamine® is a trademark of Invitrogen.
 

 

Experiment 3: Comparison of EndoFectin™ Max and Lipofectamine® 2000 in siRNA transfection

A B
 
Figure 4. Comaprison of transfection of siRNA between EndoFectin™ Max and Lipofectamine® 2000, Cell lines H1299 (A) and HEK293 (B) were transfected with either a firefly luciferase-expressing plasmid (blue bars), or co-tranfected with a firefly luciferase-expressing plasmid and an anti-luciferase siRNA (red bars). Transfection efficiencies were assessed by luciferase activity (RLU). Lipofectamine® is a trademark of Invitrogen.

 

Experiment 4: Comparison of EndoFectin™ Max and Lipofectamine® 2000 for cytotoxicity

 
Figure 5. Comparison of cytotoxicity between EndoFectin™ Max and Lipofectamine® 2000. Lipofectamine® is a trademark of Invitrogen.

 

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CRISPR-Fectin

Introducing CRISPR-Fectin™ Transfection Reagent

CRISPR-Fectin™ is optimized lipid-based transfection reagent for CRISPR-Cas9 ribonucleoprotein delivery. Compatible with our GeneHero™ Cas9 Nuclease.

Advantages

  • High transfection efficiency for Cas9 nuclease and synthetic sgRNA
  • Low toxicity to cells
  • Cost effective with as low as $0.059 per transfection reaction

 

Buy Cat.No. Product name Price Protocol MSDS
EF015 CRISPR-Fectin™ transfection reagent (1 mL) $295 Download Download
EF016 CRISPR-Fectin™ transfection reagent (3 mL) $589

 

GeneHero™ Cas9 Nuclease genome modification in HEK293 cell line. Cas9 nuclease was co-transfected with synthetic sgRNA into the HEK293 cells using CRISPR-Fectin™ transfection reagent. Three different sgRNA targeting the HUWE gene were designed and transfected with Cas9 protein respectively. The HUWE fragments were amplified from genomic DNA and subjected to the T7 endonuclease I assay. Cleaved DNA bands are indicated by red arrows.

EndoFectin Expi293

EndoFectin™ Expi293 is a powerful new transfection reagent ideally suited for transfection of the suspension cell line Expi293F. Expi293F is the most commonly used suspension HEK293 cell line for transient expression to achieve high cell density. EndoFectin™ Expi293 provides greater transfection efficiency of Expi293 cells than Expifectamine 293 over a range of cell densities, and is available as either the reagent alone or as a complete kit with 3 accompanying solutions.

Advantages

  • Specially designed for transfection of the widely-used suspension cell line Expi293F
  • Greater transfection efficiency of Expi293F cells than Expifectamine™ 293 (Figure 1).
  • Greater protein expression than Expifectamine™ 293 (Figure 2)
  • Does not require removal of serum or culture medium, or changing of medium after transfection.
  • For overexpression, knockdown, knockout, as well as high-throughput applications

 

Buy Cat.No. Product name Price Protocol MSDS
EF007 EndoFectin™ Expi293 transfection reagent (1 mL) $320 Download Download
EF009 EndoFectin™ Expi293 Transfection Kit (1L culture) $485 Download  
EF010 EndoFectin™ Expi293 Transfection Kit (10L culture) $4,155

 

Experiment 1: Comparison of EndoFectin™ Expi293 and Expifectamine™ 293 in plasmid transfection

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B
 
 
Figure 1. Comparison of transfection efficiencies of the suspension cell line Expi293F between EndoFectin™ Expi293 and Expifectamine 293. Expi293F cells were cultured in 125 ml flasks and transfected with a GFP-expressing plasmid. Transfection efficiencies were quantitated as relative fluorescence units (RFU, A) and compared visually using a fluorescence microscope (B).

 

Experiment 2: Comparison of EndoFectin™ Expi293 and Expifectamine™ 293 in protein expression

A
 
B
 
Figure 2. Comparisons of secreted (A) and intracellular (B) protein expression in suspension cell line Expi293F between the EndoFectin™ Expi293 kit (GeneCopoeia) and the Expifectamine 293 kit (Thermo Fisher). A. Expi293F cells were transfected with a Gaussia luciferase-expressing plasmid. GeneCopoeia transfection solutions or ThermoFisher enhancers were added 16-18 hours after transfection. Gaussia luciferase expression was measured in a luminometer. B. Expi293F cells were transfected with a Firefly luciferase-expressing plasmid. GeneCopoeia transfection solution 3 or Thermo Fisher enhancers were added 16-18 hours after transfection. Firefly luciferase levels were measured in a lluminometer 48 hours after transfection.

 

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EndoFectin HepG2

EndoFectin™ HepG2 is a new and potent reagent optimized for transfection of the difficult-to-transfect hepatocellular carcinoma cell line HepG2.  EndoFectin™ HepG2 provides superior transfection efficiency of HepG2 cells compared with Lipofectamine® 3000 (Figure 1) and Lipofectamine® 2000 (Figure 2).

Advantages

  • Specially formulated for the difficult-to-transfect hepatocellular carcinoma cell line HepG2
  • Greater transfection efficiency of HepG2 cells compared with Lipofectamine® 3000  (Figure 1).
  • Superior transfection efficiency of HepG2 cells compared to Lipofectamine® 2000 (Figure 2)
  • Transfection efficiency of HepG2 cells is comparable to electroporation, allowing you to achieve ultra-high levels of transfection more conveniently (Figure 3).
  • Does not require removal of serum or culture medium or removal of medium after transfection.

 

Buy Cat.No. Product name Price Protocol MSDS
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $355 Download Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $705

 

Experiment 1: Comparison of EndoFectin™ HepG2 and Lipofectamine® 3000

 

Figure 1. Transfection of HepG2 cells using either EndoFectin™ Max, Lipofectamine® 2000 (LP2000) or Lipofectamine® 3000 (LP3000). Cells were transfected with a firefly luciferase-expressing plasmid. Transfection efficiencies were assessed by luciferase activity (RLU). Note: 0.15 uL/well of Lipofectamine® 3000 was used rather than 0.2 uL/well. Lipofectamine® is a trademark of Invitrogen.

 

Experiment 2: Comparison of EndoFectin™ HepG2 and Lipofectamine® 2000

 

Figure 2. Comparison of transfection efficiencies of HepG2 cells between EndoFectin™ HepG2 and Lipofectamine® 2000 (LP2000). Top: Cells were transfected with a plasmid expressing luciferase, and quantitated in a luminometer. Bottom: HepG2 cells were transfected with a plasmid expressing GFP, and visualized using either visible light or fluorescence.

 

Experiment 3: Comparison of EndoFectin™ HepG2 and electroporation

EndoFectin™ HepG2 can also provide nearly as high transfection efficiency as electroporation, meaning that you can achieve ultra-high levels of transfection with much more convenience (Figure 3).

 

Figure 3. Comparison of transfection efficiencies of HepG2 cells between EndoFectin™ HepG2 and electroporation. HepG2 cells were electroporated with a plasmid expressing GFP, and visualized using either visible light or fluorescence.

 

Note: Lipofectamine® is a registered trademark of Invitrogen

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EndoFectin Lenti

EndoFectin™ Lenti is a robust transfection reagent, optimized specifically for lentiviral packaging. This cation-based transfection reagent delivers high transfection efficiency and high titer lentiviral packaging results, equal to or better than leading competitors (Figure 1).

Buy Cat.No. Product name Price Protocol MSDS
EF001 EndoFectin™ Lenti transfection reagent (1 mL) $245 Download Download
EF002 EndoFectin™ Lenti transfection reagent (3 mL) $489

 

EndoFectin Lenti

 

Figure 1. HEK293T cells were transfected with an EGFP-expressing plasmid using various transfection reagents as indicated. The cells were observed with an inverted fluorescence microscope 20 hours after transfection. HEK293T cells were transfected either in serum-free medium (A) or in 10% serum (B). Lipofectamine 2000® (LF2000) is a trademark of Invitrogen; FuGENE® is a trademark of Roche.

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Resources

Here you will find testimonials from customers who have had success using EndoFectin™ transfection reagents in their experiments, as well as publications that cite usage of EndoFectin™.

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Testimonials

 

Testimonial #1: Cell type: TH+ (neural cells derived differentiated from induced pluripotent stem cells)

Transfection reagent: EndoFectin™ Max

"I tested EndoFectin Max on TH+ neural cells, which are differentiated from induced pluripotent stem cells and are hard to transfect. EndoFectin Max transfected my cells at least as well or better than Lipofectamine 3000. EndoFectin Max is also easier to use. I transfect the cells overnight and, using other transfection methods, I risked cell loss due to lack of nutrients. This is not an issue with  EndoFectin Max, because it allows me to keep my cells in complete media during the entire incubation. I will get the entire lab on board to try EndoFectin Max".

          -Gabriela Novak, University of California at San Francisco

 

Testimonal #2. Cell type: 10T1/2

Transfection reagent: EndoFectin™ Max

"Endo Fectin max is a good transfection reagent as I compared other vendors for difficult to transfect cells. It’s less toxic to the cells. Expression of the transfected protein is comparable or slightly higher to other vendors and I recommend using Endo Fectin max"

           –Amrendra K Ajay, Harvard Medical School

 

Testimonal #3. Cell type: Vero

Transfection reagent: EndoFectin™ Max

"I did use your Endofectin Max reagent several times and was quite impressed. Not having to remove the transfection mix is a nice touch.  I compared it to Lipofectamine® 2000 as well as Fugene® 6 and I got about 2x transfection efficiency over each.  I'm working with Vero cells which are hard to transfect."

           – Jeff Davis, Arisan Therapeutics

 

Testimonal #4. Cell type: HEK293

Transfection reagent: EndoFectin™ Max

"HEK293 cells were transfected with either EndoFectin Max or Lipofectamine 3000 with a luciferase-expressing plasmid. The cells transfected with EndoFectin exhibited 2-3 fold greater luminescence values over the Lipofectamine-treated cells"

           –Angel Cid-Arregui, German Cancer Research Center, Heidelberg, Germany

 

Testimonal #5.

Transfection reagent: EndoFectin™ Lenti

"Endofectin Lenti has enabled us at the Viral Pseudotype Unit (VPU) to dramatically increase the titres of our influenza lentiviral pseudotypes. With titres exceeding 10^9 RLU/ml we can perform large scale screening projects with a single batch of virus."

           –George Carnell, Universities of Greenwich and Kent, Kent, United Kingdom

 

Testimonial #6: Cell type: HUVEC

Transfection reagent: EndoFectin™ Max

“We used EndoFectin Max on HUVEC cells. We can say that EndoFectin Max works well with this cell line relative to other transfection reagents.”

-Marcin Magierowski,Jagiellonian University Medical College, Krakow, Poland

 

Testimonial #7: Cell type: HEK293

Transfection reagent: EndoFectin™ Max

“I used EndoFectin Max for HEK293 cells. It has good efficiency and low toxicity.  I am regularly engineering cell lines and need a good reagent like this one.”

-Janice Thomas, Oncoveda Cancer Research Center, Medical Diagnostic Laboratories, LLC

 

Testimonial #8: Cell type: Mouse embryonic stem cells

Transfection reagent: EndoFectin™ Max

“We have been using EndoFectin Max for several months now for a variety of transfections (e.g. dsRNA, mRNA, plasmid DNA) in mouse ESCs (D3 cell line) and mESC-differentiated fibroblasts, and have been very pleased with our results…high efficiency and little to no toxicity. I'd definitely recommend this product to others.”

-William D'Angelo, The University of Southern Mississippi

 

Testimonial #9: Cell type: HEK293T

Transfection reagent: EndoFectin™ Max

“I got 65% transfection efficiency with EndoFectin Max on HEK293T cells. I would recommend this product to others.”

-Hemant Raj Joshi, Graduate Student, University of Utah

 

Testimonial #10: Cell type: Rat primary hippocampal neurons

Transfection reagent: EndoFectin™ Max

"My lab has had a chance to transfect primary hippocampal neurons with the sample you sent, and so far, it works really well!

From the attached image you'll see that the transfection might appear low…but for our imaging studies we need to see single neurons in their entirety. With too many transfectants, this becomes a problem. We typically see between 5-10% transfection rates of these cells with the sample. This is also about 10X the transfection rate compared to Lipofectamine 2000."

           – Michael Silverman, Assoc. Professor, Simon Fraser University

  
  Rat neurons after 11 days of in vitro culture transfected with a GFP-expressing plasmid using EndoFectin™ Max (green) . Cells were visualized for fluoresence 24 hours after transfection. Blue: DAPI staining for nuclei.  

 

Want to say something about our transfection reagents? Please email us at inquiry@genecopoeia.com.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

We value your feedback. Please let us know

1.What kind of cells are you using?
2.What kind of challenges you are facing?
3.What are the results from using our transfection reagents?
4.Send an image from your transfection experiments.
 

After we receive your comments, we will send you a specially-designed thank-you gift. Please include your contact information in the email you send.

   

 

Publications citing GeneCopoeia's EndoFectin™ Transfection Reagents

 

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