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    Mouse ROSA26 safe harbor gene knock-in kits and clones

    Genome-TALER™mouse ROSA26 safe harbor gene knock-in kit
    Genome-TALER™mouse ROSA26 safe harbor gene knock-in kit
    Genome-TALER™ mouse ROSA26 safe harbor gene knock-in kit (without donor)
    Genome-TALER™ mouse ROSA26 safe harbor gene knock-in kit (without donor)
    GeneHero™mouse ROSA26 safe harbor gene knock-in kit
    GeneHero™mouse ROSA26 safe harbor gene knock-in kit
    GeneHero™ mouse ROSA26 safe harbor gene knock-in kit (without donor)
    GeneHero™ mouse ROSA26 safe harbor gene knock-in kit (without donor)
    Cas9 ROSA26 knockin donor clone-Puro
    Cas9 ROSA26 knockin donor clone-Puro
    Cas9 ROSA26 knockin donor clone-Hygro
    Cas9 ROSA26 knockin donor clone-Hygro
    Cas9 ROSA26 knockin donor clone-Neo
    Cas9 ROSA26 knockin donor clone-Neo

    Genome-TALER™mouse ROSA26 safe harbor gene knock-in kit Genome-TALER™ mouse ROSA26 safe harbor gene knock-in kit (without donor) GeneHero™mouse ROSA26 safe harbor gene knock-in kit GeneHero™ mouse ROSA26 safe harbor gene knock-in kit (without donor) Cas9 ROSA26 knockin donor clone-Puro Cas9 ROSA26 knockin donor clone-Hygro Cas9 ROSA26 knockin donor clone-Neo

    Price: $1,395.00 $1,195.00 $1,395.00 $1,195.00 $495.00 $495.00 $495.00
    Catalog#:
    • SH079
    • SH075
    • SH054
    • SH050
    • SH350
    • SH351
    • SH352
    Size: Cas9 ROSA26 knockin donor clone Cas9 ROSA26 knockin donor clone Cas9 ROSA26 knockin donor clone
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    Introduction

    ROSA26 (also known as ROSAβgeo26 locus) in the mouse genome is first found in chromosome 6 in one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. This locus expresses one coding transcript and two noncoding transcripts, and only the non-coding transcripts are disrupted by the insertion. While pups homozygous for the insertion are born at slightly lower frequency than heterozygous pups, homozygotes appeared to develop normally and were fertile. Thererore, the “Rosa26” locus has since been used as a transgene insertion site that causes no apparent adverse effects on fitness, and permits stable gene expression.

    ROSA26-specific TALENs or CRISPR-Cas9 can generate a DNA double-strand break (DSB) at ROSA26 in the mouse genome, stimulating natural DNA repair mechanisms. In the presence of ROSA26 ORF knockin clones, homologous recombination (HR) occurs and integrates DNA fragments from knockin clones into the safe harbor locus.