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Mouse ROSA26 safe harbor gene knock-in kits and clones

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Introduction

ROSA26 (also known as ROSAβgeo26 locus) in the mouse genome is first found in chromosome 6 in one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. This locus expresses one coding transcript and two noncoding transcripts, and only the non-coding transcripts are disrupted by the insertion. While pups homozygous for the insertion are born at slightly lower frequency than heterozygous pups, homozygotes appeared to develop normally and were fertile. Thererore, the “Rosa26” locus has since been used as a transgene insertion site that causes no apparent adverse effects on fitness, and permits stable gene expression.

ROSA26-specific TALENs or CRISPR-Cas9 can generate a DNA double-strand break (DSB) at ROSA26 in the mouse genome, stimulating natural DNA repair mechanisms. In the presence of ROSA26 ORF knockin clones, homologous recombination (HR) occurs and integrates DNA fragments from knockin clones into the safe harbor locus.

 
 
 
 

 

Mouse ROSA26 safe harbor knockin kits

Buy Catalog # Product Description Price
SH079 Genome-TALER™
mouse ROSA26 safe harbor gene knock-in kit
Includes:
ROSA26 TALEN pair (TN-ROSA26)
ROSA26 donor cloning vector (DC-DON-SH02)
ROSA26 positive control donor (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)
$1395
SH075 Genome-TALER™
mouse ROSA26 safe harbor gene knock-in kit (without donor)
Includes:
ROSA26 TALEN pair (TN-ROSA26)
ROSA26 positive control (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)
$1195
SH054 GeneHero™
mouse ROSA26 safe harbor gene knock-in kit
Includes:
ROSA26 sgRNA/Cas9 expression clone (MCP-ROSA26-CG01)
ROSA26 donor cloning vector (DC-DON-SH02)
ROSA26 positive control donor (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)
$1395
SH050 GeneHero™
mouse ROSA26 safe harbor gene knock-in kit (without donor)
Includes:
ROSA26 sgRNA/Cas9 expression clone (MCP-ROSA26-CG01)
ROSA26 positive control (DC-RFP-SH02)
knock-in verification primer pairs (MQPROSHR)
$1195

 

Mouse ROSA26 Safe Harbor knock-in ORF clones

Catalog # Product Description Price
Variable Mouse ROSA26 knock-in ORF donor clones Mouse ROSA26 knock-in ORF donor clone containing CMV-driven ORF of customer's gene of interest

 

Mouse ROSA26 Safe Harbor knockin CRISPR-Cas9 clones

Important Note: The vector-specific knock-in verification primers provided with the Mouse ROSA26 Safe Harbor knock-in kits (catalog numbers: SH079, SH075, SH054, and SH050) are not compatible with the Mouse ROSA26 Safe Harbor knock-in CRISPR-Cas9 clones in the table below. If you purchase these clones with our Mouse Safe Harbor knock-in kits, you will be provided with clone-specific knock-in verification primers.

 

 

Buy Catalog # Product Application Selection marker Promoter Cas9 integration site Delivery format Price
SH350 Cas9 ROSA26 knock-in donor clone To knock-in Cas9 nuclease gene to mouse ROSA26 safe harbor site using CRISPR or TALEN Puro CBh ROSA26 Bacterial stock or transfection-ready DNA $495
SH351 Cas9 ROSA26 knock-in donor clone Hygro CBh ROSA26 $495
SH352 Cas9 ROSA26 knock-in donor clone Neo CBh ROSA26 $495