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    Luciferase Assays and Reporters

    Luciferases are widely-used reporters of biological activity, due to their bright signals and nearly non-existent background luminescence. GeneCopoeia’s Secrete-Pair™ system offers the unique advantage of a secreted dual-reporter luminescence system, which allows reporter assays in live cells. Combined with choices for traditional Firefly and Renilla luciferase assay kits to provide compatibility with multiple systems, as well as superior quality luciferase reporter clones for miRNA targets, promoters, and transcriptional response elements, GeneCopoeia’s luciferase products provide you with a wealth of tools for luminescence reporter analysis.

    Refer to the table below to compare features between different luciferase kit options.

     Gaussia

     luciferase assays

    Secrete-Pair™ Gaussia luciferase (GLuc) assay kit (detects GLuc)
    Secrete-Pair™ dual luminescence assay kit (detects GLuc and SEAP)
         

     Firefly/Renilla

     luciferase assays

    Luc-Pair™ duo-luciferase assay kit 2.0 (for enhanced stability)
    Luc-Pair™ duo-luciferase HS assay kit (for high sensitivity)
    Luc-Pair™ duo-luciferase HT assay kit (for high throughput)
    Luc-Pair™ firefly luciferase HS assay kit (for high sensitivity)
    Luc-Pair™ firefly luciferase HT assay kit (for high throughput) 
    Luc-Pair™ Renilla luciferase HS assay kit (for high sensitivity)
    Luc-Pair™ Renilla luciferase HT assay kit (for high throughput) 
     Reporter clones GLuc-ON™ promoter  reporter  clones
    miTarget™ 3′ UTR miRNA target clones
    GLuc-ON™ transcriptional response element reporter

      Vector Type Luc-Pair™ Luciferase 
    Assay Kits
    Secrete-Pair™ Luciferase 
    Assay Kits
    Duo luciferase assay kit 2.0 Duo luciferase HS assay kit Duo luciferase HT assay kit Firefly luciferase HS assay kit Renilla luciferase HS assay kit  Dual luminescence assay kit Gaussia luciferase assay kit
    Reporter Genes   Firefly and Renilla luciferase Firefly and Renilla Luciferase Firefly and Renilla luciferase Firefly  luciferase Renilla Luciferase Gaussia luciferase/ decreted alkaline phosphatase Gaussia luciferase
    Key Feature   For enhanced stability For high sensitivity For high throughput For high sensitivity For high sensitivity For secreted reporters For secreted reporters
    GLuc-ON™ Promoter Reporter Clones pEZX-PG04                
    pEZX-PG02                
    pEZX-LvPG04                
    pEZX-LvPG02                 
    miTarget™ 3′ UTR miRNA Target Clones pEZX-MT05                 
    pEZX-MT06                
    GLuc-ON™ Transcriptional Response Element Reporter pEZX-PG02              
    GeneCopoeia’s Vector Options pEZX-FR01*                
    pEZX-FR02*                
    pEZX-FR03*                
    pEZX-GN01*                
    pEZX-GN03*                
    pEZX-GA01*                  
    pEZX-GA02*                
    pEZX-GA03*                  
    pEZX-LvGN01*                  
    pEZX-LvGA01*                  
    Promega’s Vector Options pGL4 luciferase reporter vectors                
    pmirGLO dual-luciferase miRNA target expression vectors                

    * To order, please visit the corresponding luciferase assay kit’s web page.