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    Luc-Pair™ Firefly Luciferase HS Assay Kit

    Luc-Pair™ Firefly Luciferase HS Assay Kit
    Luc-Pair™ Firefly Luciferase HS Assay Kit
    Luc-Pair™ Firefly Luciferase HS Assay Kit
    Luc-Pair™ Firefly Luciferase HS Assay Kit
    Luc-Pair™ Firefly Luciferase HS Assay Kit
    Luc-Pair™ Firefly Luciferase HS Assay Kit
    Luc-Lysis buffer (10×)
    Luc-Lysis buffer (10×)

    Luc-Pair™ Firefly Luciferase HS Assay Kit Luc-Pair™ Firefly Luciferase HS Assay Kit Luc-Pair™ Firefly Luciferase HS Assay Kit Luc-Lysis buffer (10×)

    Price: $77.00 $125.00 $375.00 $40.00
    Catalog#:
    • LF007
    • LF008
    • LF009
    • LF003-01
    Size: 100rxns 300rxns 1000rxns 10ml
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    – High sensitivity

     

    Introduction

    GeneCopoeia’s Luc-Pair™ Firefly Luciferase Assay Kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) activity. The Luc-Pair™ Firefly Luciferase HS Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.) using micro-plate or single-tube luminometers, and is compatible with GeneCopoeia, Promega and many other Fluc vectors.

    Advantages

    • High sensitivity. The reagents have been developed so that the signals for Firefly luciferase exhibit greatest sensitivity (Figure 1).
    • Versatility. The system has been designed for assays with many different eukaryotic (vertebrates, lower invertebrates) cells using micro-plates or single-tube luminescence reader.
    • Low background. The system produces very limited background luminescence. No subtraction is required from readings.
    • Reproducibility. This system is designed to yield reliable, linear results for a concentration range over several orders of magnitude.

     

    GeneCopoeia also offers the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (for enhanced stability) and  Luc-Pair™ Duo-Luciferase HS Assay Kit (for high sensitivity) for applications requiring luciferase readings.

     

    Figure 1.  Activity of Firefly luciferase signals using GeneCopoeia Luc-Pair™ Firefly Luciferase HS Assay Kit. HEK 293 cells were transfected with Promega pGL4.13 reporter vector for 48 hours. The FLuc (FLuc-GeneCopoeia) activity was measured as described in the procedure. Promega’s Luciferase Assay System was used (FLuc-Promega) in comparison.

    Figure 2.  Signal stability of luciferase using GeneCopoeia Luc-Pair Firefly-Luciferase (FLuc) HS Assay Kit. HEK 293 cells were transfected with Promega pGL4.13 reporter vector for 48 hours. The FLuc (FLuc-GeneCopoeia) activity was measured as described in the procedure. A sample injector was used to dispense the Fluc Working Solution, set shaking to mix for 1 sec, and counting was started immediately for 20 sec.