Secrete-Pair™ Dual Luminescence Assay Kit

Secrete-Pair Dual Luminescence Assay Kit
Secrete-Pair Dual Luminescence Assay Kit
Secrete-Pair Dual Luminescence Assay Kit
Secrete-Pair Dual Luminescence Assay Kit
Secrete-Pair Dual Luminescence Assay Kit
Secrete-Pair Dual Luminescence Assay Kit
Secrete-Pair <em>Gaussia</em> Luciferase Assay Kit
Secrete-Pair Gaussia Luciferase Assay Kit
Secrete-Pair <em>Gaussia</em> Luciferase Assay Kit
Secrete-Pair Gaussia Luciferase Assay Kit

Secrete-Pair Dual Luminescence Assay Kit Secrete-Pair Dual Luminescence Assay Kit Secrete-Pair Dual Luminescence Assay Kit Secrete-Pair Gaussia Luciferase Assay Kit Secrete-Pair Gaussia Luciferase Assay Kit

Price: $139.00 $379.00 $959.00 $77.00 $399.00
Catalog#:
  • LF031
  • LF032
  • LF033
  • LF061
  • LF062
Size: 100rxns 300rxns 1000rxns 100rxns 1000rxns
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Introduction

Secrete-Pair™ Gaussia Luciferase Dual and Single Luminescence Assay Kits

GeneCopoeia’s Secrete-Pair™ Dual Luminescence Assay Kit is designed to analyze the activities of Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) in a dual-reporter system. Both GLuc and SEAP are secreted reporter proteins, permitting detection without cell lysis. Secrete-Pair measures dual reporter signals and allows transfection normalization. A single GLuc reporter system is also available.

Advantages

• Live cell assays. Secreted GLuc and SEAP make cell lysis unnecessary. The same sample can be assayed multiple times for different time points, environmental conditions, etc., reducing sample-to-sample variation. Ideal for high-throughput applications.

• Dual-reporter detection. SEAP allows normalization of GLuc signal for greater accuracy.

• Sensitive and robust system. GLuc is 1,000 times more sensitive than firefly and Renilla luciferases.

• Flexible assay conditions. Two robust buffer conditions are provided for GLuc assays: 1) Buffer for greater stability retains more than 90% of signal within the first 10 minutes, extending the half-life of luminescence to 30 minutes; 2) Buffer for higher sensitivity for detecting low GLuc expression.

• Clone and vector compatibility. Compatible with GeneCopoeia’s GLuc-ON™ promoter reporter clones, miTarget™ miRNA target clones, GLuc-ON™ Transcriptional Response Element (TRE) clones, and cloning vectors.