SureScript™ First-Strand cDNA Synthesis Kit

Get $50 Amazon gift card* w/ purchase of BlazeTaq qPCR Mix + SureScript Synthesis Kit

 

A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from almost any source of RNA

  • Efficient and easy
  • Reliable
  • Cost efficient

The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.

The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.

+ Receive $50 Amazon gift card with purchase of BlazeTaq™ SYBR Green qPCR Mix 2.0 + SureScript™ First-Strand cDNA Synthesis Kit!*

 

SureScript™ First-Strand cDNA Synthesis Kit

Buy Catalog# Product Description Price
QP056 SureScript™ First-Strand cDNA Synthesis Kit (20 rxn) M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo (dT), Random Primer, and ddH2O $129
QP057 SureScript™ First-Strand cDNA Synthesis Kit (60 rxn) M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo (dT), Random Primer, and ddH2O $285

 

 

 

BlazeTaq™ SYBR® Green qPCR Mix Kits 2.0 (with or without ROX)

Buy Catalog# Product Description Price
QP031 BlazeTaq™ SYBR Green qPCR Mix (200 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $198
QP032 BlazeTaq™ SYBR Green qPCR Mix (600 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $465
QP034 BlazeTaq™ SYBR Green qPCR Mix (1000 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $760
QP041 BlazeTaq™ SYBR Green qPCR Mix (200 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $198
QP042 BlazeTaq™ SYBR Green qPCR Mix (600 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $465
QP044 BlazeTaq™ SYBR Green qPCR Mix (1000 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $760
  Variable All-in-One™ qPCR Primer
(200 reactions)
Validated gene-specific for human, mouse and rat to ensure specificity and sensitivity
 

 

 

SureScript™ First-Strand Synthesis Kit in action

The SureScript™ First Strand Synthesis Kit can be used in combination with the BlazeTaq™ SYBR Green qPCR Master Mix as the first step of the two-step RT-qPCR system to convert mRNA into cDNA; the cDNA can be subsequently aliquoted, stored or used for qPCR analysis.

two-step RT-qPCR amplification

Figure 1. Amplification of actin-β using the BlazeTaq™ two-step RT-qPCR system. A 10-fold dilution series of RNA extracts from HeLa cells were used for cDNA conversion with the SureScript™ First-Strand cDNA Synthesis Kit. The actin-β cDNA was amplified with BlazeTaq™ SYBR® Green qPCR mix 2.0 with amounts ranging from ~100 ng to ~1 pg.

 

 

two-step RT-qPCR

Figure 2. Comparison of the real-time amplication plots of the BlazeTaq Two-Step RT-qPCR System (red) with competitor A’s (A) and competitor B’s (B) products (blue). A 10-fold dilution series of B2M gene from total RNA of Hela cell extract with amounts ranging from 100 ng to 1 pg.

 

 

* Offer is valid in the US & Canada only. Must buy both items to receive gift card. Limit 1 offer per customer per order. Offer ends July 31, 2019