A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from almost any source of RNA
- Efficient and easy
- Reliable
- Cost efficient
The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.
The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
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SureScript™ First-Strand cDNA Synthesis Kit
Product details
SureScript™ First-Strand Synthesis Kit in action
The SureScript™ First Strand Synthesis Kit can be used in combination with the BlazeTaq™ SYBR Green qPCR Master Mix as the first step of the two-step RT-qPCR system to convert mRNA into cDNA; the cDNA can be subsequently aliquoted, stored or used for qPCR analysis.

Figure 1. Amplification of actin-β using the BlazeTaq™ two-step RT-qPCR system. A 10-fold dilution series of RNA extracts from HeLa cells were used for cDNA conversion with the SureScript™ First-Strand cDNA Synthesis Kit. The actin-β cDNA was amplified with BlazeTaq™ SYBR® Green qPCR mix 2.0 with amounts ranging from ~100 ng to ~1 pg.

Figure 2. Comparison of the real-time amplication plots of the BlazeTaq Two-Step RT-qPCR System (red) with competitor A’s (A) and competitor B’s (B) products (blue). A 10-fold dilution series of B2M gene from total RNA of Hela cell extract with amounts ranging from 100 ng to 1 pg.
Resources
Resources
Manuals
SureScript™ First-Strand cDNA Synthesis Kit- User Manual
Publications
View selected publications citing GeneCopoeia's SureScript™ First-Strand cDNA Synthesis Kit from the recent literature
2022
- Qin, S., et al. (2022). The comprehensive expression and functional analysis of m6A modification “readers” in hepatocellular carcinoma. Aging (Albany NY) doi: 10.18632/aging.204217 [SureScript™ First-Strand cDNA Synthesis Kit]
- Wang, H-F., et al. (2022). Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma. Cancers (Basel) doi: 10.3390/cancers14174151 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR Green qPCR mix 2.0]
- Yin, S., et al. (2022). Peptide OM-LV20 protects astrocytes against oxidative stress via the ‘PAC1R/JNK/TPH1’ axis. J Biol Chem doi: 10.1016/j.jbc.2022.102429 [SureScript™ First-Strand cDNA Synthesis Kit]
- Pan, Y-Q., et al. (2022). Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma. Front Oncol doi: 10.3389/fonc.2022.1071100 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR Mix 2.0]
- Deng, H.X., et al. (2022). Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway. Anal Cell Pathol (Amst) doi: 10.1155/2022/4588999 [SureScript™ First-Strand cDNA Synthesis Kit]
- Dongm, X.M., et al. (2022). Identification of optimal reference genes for gene expression normalization in human osteosarcoma cell lines under proliferative conditions. Front Genet doi: 10.3389/fgene.2022.989990 [SureScript™ First-Strand cDNA Synthesis Kit]
- Lin, C., et al. (2022). Integrating RNA-seq and scRNA-seq to explore the biological significance of NAD + metabolism-related genes in the initial diagnosis and relapse of childhood B-cell acute lymphoblastic leukemia. Front Immunol doi: 10.3389/fimmu.2022.1043111 [SureScript™ First-Strand cDNA Synthesis Kit]
- Li, z.h., et al. (2022). Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes. Int J Mol Sci doi: 10.3390/ijms232314607 [SureScript™ First-Strand cDNA Synthesis Kit]
- Jiang, H., et al. (2022). Bioinformatics identification and validation of biomarkers and infiltrating immune cells in endometriosis. Front Immunol doi: 10.3389/fimmu.2022.944683 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Zhao, J.D., et al. (2022). Construction of N-7 methylguanine-related mRNA prognostic model in uterine corpus endometrial carcinoma based on multi-omics data and immune-related analysis. Sci Rep doi: 10.1038/s41598-022-22879-6 [SureScript™ First-Strand cDNA Synthesis Kit]
- Zhang, Q.Y., et al. (2022). Screening and validation of lymph node metastasis risk-factor genes in papillary thyroid carcinoma. Front Endocrinol (Lausanne) doi: 10.3389/fendo.2022.991906 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Lei, L., et al. (2022). Comprehensive Diagnostics of Diabetic Nephropathy by Transcriptome RNA Sequencing. Diabetes Metab Syndr Obes doi: 10.2147/DMSO.S371026 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Pan, Y-Q., et al. (2022). Prognostic value of lncRNAs related to fatty acid metabolism in lung adenocarcinoma and their correlation with tumor microenvironment based on bioinformatics analysis. Front Oncol doi: 10.3389/fonc.2022.1022097 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Liu, Q.S., et al. (2022). Construction and Validation of Pyroptosis-Related lncRNA Prediction Model for Colon Adenocarcinoma and Immune Infiltration Analysis. Dis Markers doi: 10.1155/2022/4492608 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Liu, C., et al. (2022). Prognostic Modeling of Lung Adenocarcinoma Based on Hypoxia and Ferroptosis-Related Genes. J Oncol doi: 10.1155/2022/1022580 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ One-Step SYBR Green RT-qPCR Kit]
- Sun, L.H., et al. (2022). The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission. Oxid Med Cell Longev doi: 10.1155/2022/3639302 [SureScript™ First-Strand cDNA Synthesis Kit]
- Tan, L.J., et al. (2022). Peripheral Transplantation of Mesenchymal Stem Cells at Sepsis Convalescence Improves Cognitive Function of Sepsis Surviving Mice. Oxid Med Cell Longev doi: 10.1155/2022/6897765 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Bai, R.J., et al. (2022). Exploring the biological function of immune cell-related genes in human immunodeficiency virus (HIV)-1 infection based on weighted gene co-expression network analysis (WGCNA). BMC Med Genomics doi: 10.1186/s12920-022-01357-y [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Luo, S-M., et al. (2022). Dexmedetomidine exerts an anti-inflammatory effect via α2 adrenoceptors to alleviate cognitive dysfunction in 5xFAD mice. Front Aging Neurosci doi: 10.3389/fnagi.2022.978768 [SureScript™ First-Strand cDNA Synthesis Kit]
- Bai, F., et al. (2022). Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy. Front Genet doi: 10.3389/fgene.2022.965816 [SureScript™ First-Strand cDNA Synthesis Kit]
- Xu, X., et al. (2022). ECM-inspired 3D printed polyetherimide scaffold with Arg-Gly-Asp peptides for the improvement of bioactivity and osteogenic differentiation of osteoblasts. Materials Today Communications doi: 10.1016/j.mtcomm.2022.103166 [SureScript™ First Strand cDNA Synthesis Kit]
- Huang, Z., et al. (2022). Mechanism of ketotifen fumarate inhibiting renal calcium oxalate stone formation in SD rats. Biomedicine & Pharmacotherapy doi: 10.1016/j.biopha.2022.113147 [SureScript™ First Strand cDNA Synthesis Kit]
- Ma, M., et al. (2022). Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments. Cancer Medicine doi: 10.1002/cam4.4897 [SureScript™ First Strand cDNA Synthesis Kit]
- Zha, W., et al. (2022). Genome-wide identification of long non-coding (lncRNA) in Nilaparvata lugens’s adaptability to resistant rice. Zoological Science doi: 10.7717/peerj.13587 [SureScript™ First Strand cDNA Synthesis Kit]
- Bai, Z., et al. (2022). ERCC5, HES6 and RORA are potential diagnostic markers of coronary artery disease. FEBS open bio doi: 10.1002/2211-5463.13469 [SureScript™ First Strand cDNA Synthesis Kit]
2021
2020
2019
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