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MycoGuard™ Mycoplasma PCR Detection Kit

Introduction

– Quick detection of mycoplasma contamination

Guarding your cells from mycoplasma contamination requires periodic testing to make sure the cells are free of mycoplasma contamination and are reliable for experiments. GenCopoeia's MycoGuard Mycoplasma PCR Detection Kit is a quick, simple and sensitive PCR-based mycoplasma detection kit, designed to serve such a purpose. The kit contains an optimized MycoGuard master mix, ultrapure water and a positive control. The primers in the MycoGuard master mix are highly specific to the conserved rDNA region in the mycoplasma genomes and can detect all well-known mycoplasma genera, including the commonly encountered ones in cell cultures, such as M. arginini, M. arthritidis, M. bovis, M. fermentans, M. genitalium, M. hominis, M. hyorhinis, M. neurolyticum, M. orale, M. pirum, M. pneumoniae, M. pulmonis, M. salivarium, and U. urealyticum. Mycoplasma positive samples can be easily recognized by a distinctive PCR product ranging in size from 150 to 400 bp.

Key advantages

Simple

  • The PCR reaction is ready to go
  • No need to pre-treat your cell culture medium

Fast

  • Get results in as short as 2 hours

Sensitive

  • Detect mycoplasma from 0.03 µl of cell culture medium

 

Figure 1. Example of mycoplasma PCR

M: 100 bp DNA ladder 
C: Negative control
1-5: non-treated cell culture medium, 0.03, 0.1, 0.3, 1.0, and 3.0 ml, respectively
6-10: boiled cell culture medium, 0.03, 0.1, 0.3, 1.0, and 3.0 ml, respectively
*5, 10: serum proteins and other components in the cell culture medium can inhibit the PCR reactions