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GLuc-ON™ Promoter Reporter Clones

Introduction

Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of about 39,500 human, 28,700 mouse and 17,500 rat promoters using live cell assays.GLuc-ON promoter clones can be ordered as pre-designed or custom-built in one of our robust vector systems. Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses GLuc, mCherry, or GFP as the promoter reporter. The dual-reporter system uses GLuc as the promoter reporter and SEAP (secreted Alkaline Phosphatase) as the internal control for signal normalization. GLuc and SEAP-containing promoter-reporter clones are fully compatible with GeneCopoeia's Secrete-Pair™ dual luminescence assays kits.

Advantages

  • Live cell assays. Naturally secreted GLuc and SEAP reporters makes lysis of the cells unnecessary. Reduces the number of samples required and sample-to-sample variation for applications such as pulse chase analysis and time-courses.
  • Dual secreted reporter system. SEAP enables transfection normalization for accurate across-sample comparison
  • High sensitivity. GLuc is 1,000-fold more sensitive than firefly and Renilla luciferases
  • Convenience. All promoter clones are transfection-ready

 

Order pre-designed promoter clones

To order pre-designed promoter clones, search almost 40,000 human, 30,000 mouse, and 18,000 rat clones in your choice of vector.

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Custom promoter clone services

Don't see what you need in our collection of pre-designed promoter clones? Contact us for our custom services. Please complete our Custom Promoter Clone Inquiry and Quotation form, email inquiry@genecopoeia.com, or call: 866-360-9531.

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Promoter lentivirus

GeneCopoeia also offers ready-to-use lentiviral particles expressing promoter-reporters as a custom service. 

 

Vector choices

GLuc-ON™ promoter-reporter clones are available in a variety of vector types, which enable you to choose either the type of reporter, (GLuc, eGFP, etc.), whether or not you want a tracking gene for normalization, selection marker, and non-viral or lentiviral. Refer to the table below to discover the distinguishing features of Gluc-ON™ promoter-reporter vectors.

Vector Reporter gene Tracking gene Selection marker Vector type
pEZX-PG04** Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin Non-viral
pEZX-PG02** Gaussia luciferase (GLuc) N/A* Puromycin Non-viral
pEZX-PF02 eGFP N/A* Puromycin Non-viral
pEZX-PM02 mCherry N/A* Puromycin Non-viral
pEZX-LvPG04** Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin HIV Lentiviral
pEZX-LvPG02** Gaussia luciferase (GLuc) N/A* Puromycin HIV Lentiviral
pEZX-LvPF02 eGFP N/A* Puromycin HIV Lentiviral
pEZX-LvPM02 mCherry N/A* Puromycin HIV Lentiviral
pEZX-LvPM03 tdTomato N/A* Puromycin HIV Lentiviral

* A separate vector is available for SEAP expression.

** These Gaussia luciferase cloning vectors are available for purchase. 

 

Control clones and SEAP expression clone

For most experiments with promoter-reporter clones, GeneCopoeia recommends including both positive and negative controls. See below to choose from a larger number of control clones. You can also purchase an SEAP expression clone, to normalize signals for differences in transfection efficiency for single reporter vectors.

Buy Catalog Product Name Description Description2 Price
GAPDH-LvPF02 GAPDH positive control clone House keeping gene promoter positive control in single GFP reporter lentiviral vector GAPDH positive control clone $595
GAPDH-LvPG02 GAPDH positive control clone House keeping gene promoter positive control in single-reporter lentiviral vector GAPDH positive control clone $595
GAPDH-LvPG04 GAPDH positive control clone House keeping gene promoter positive control in dual-reporter lentiviral vector GAPDH positive control clone $745
GAPDH-LvPM02 GAPDH positive control clone House keeping gene promoter positive control in single mCherry reporter lentiviral vector GAPDH positive control clone $595
GAPDH-LvPM03 GAPDH positive control clone House keeping gene promoter positive control in single tdTomato reporter lentiviral vector GAPDH positive control clone $595
GAPDH-PF02 GAPDH positive control clone House keeping gene promoter positive control in single GFP reporter vector GAPDH positive control clone $375
GAPDH-PG02 GAPDH positive control clone House keeping gene promoter positive control in single-reporter vector GAPDH positive control clone $375
GAPDH-PG04 GAPDH positive control clone House keeping gene promoter positive control in dual-reporter vector GAPDH positive control clone $495
GAPDH-PM02 GAPDH positive control clone House keeping gene promoter positive control in single mCherry reporter vector GAPDH positive control clone $375
NEG-LvPF02 Negative control clone Non-promoter sequence negative control in single GFP reporter lentiviral vector Negative control clone $595
NEG-LvPG02 Negative control clone Non-promoter sequence negative control in single-reporter lentiviral vector Negative control clone $595
NEG-LvPG04 Negative control clone Non-promoter sequence negative control in dual-reporter lentiviral vector Negative control clone $745
NEG-LvPM02 Negative control clone Non-promoter sequence negative control in single mCherry reporter lentiviral vector Negative control clone $595
NEG-LvPM03 Negative control clone Non-promoter sequence negative control in single tdTomato reporter lentiviral vector Negative control clone $595
NEG-PF02 Negative control clone Non-promoter sequence negative control in single GFP reporter vector Negative control clone $375
NEG-PG02 Negative control clone Non-promoter sequence negative control in single-reporter vector Negative control clone $375
NEG-PG04 Negative control clone Non-promoter sequence negative control in dual-reporter vector Negative control clone $495
NEG-PM02 Negative control clone Non-promoter sequence negative control in single mCherry reporter vector Negative control clone $375
SEAP-LvPA02 Secreted alkaline phosphatase (SEAP) expression clone Express SEAP in a lentiviral vector. Can be used for co-transduction with a promoter clone pEZX-PA02 $595
SEAP-PA01 Secreted alkaline phosphatase (SEAP)
Expression clone
Express SEAP.
Can be used for co-transfection with promoter clone
pEZX-PA01 $395

 

Cloning vectors

GeneCopoeia also offers Gaussia luciferase (Gluc) reporter vectors shown below. Click on the name to view the vector map.

Buy Catalog# Product Description Promoter Reporter gene Tracking gene Selection marker Size Price
ZX101 pEZX-GN01 Gaussia luciferase (Gluc) reporter cloning vector N/A* Gluc N/A* Puromycin (10µg) $375
ZX102 pEZX-GN03 Gaussia luciferase (Gluc) reporter cloning vector miniCMV Gluc N/A* Puromycin (10µg) $375
ZX103 pEZX-GA01 Gaussia luciferase (Gluc) and secreted alkaline phosphatase (SEAP) reporter cloning vector N/A* Gluc SEAP Puromycin (10µg) $495
ZX104 pEZX-GA02 Gaussia luciferase (Gluc) and secreted alkaline phosphatase (SEAP) reporter cloning vector SV40 Gluc SEAP Neomycin (10µg) $495
ZX105 pEZX-GA03 Gaussia luciferase (Gluc) and secreted alkaline phosphatase (SEAP) reporter cloning vector miniCMV Gluc SEAP Puromycin (10µg) $495
ZX106 pEZX-LvGN01 Gaussia luciferase (Gluc) reporter cloning vector (lentiviral) N/A* Gluc N/A* Puromycin (10µg) $375
ZX107 pEZX-LvGA01 Gaussia luciferase (Gluc) and secreted alkaline phosphatase (SEAP) reporter cloning vector (lentiviral) N/A* Gluc SEAP Puromycin (10µg) $495
ZX108 pEZX-LvFL01 eGFP reporter cloning vector (lentiviral) N/A eGFP N/A Puromycin (10µg) $375
ZX109 pEZX-LvFL02 mCherry reporter cloning vector (lentiviral) N/A mCherry N/A Puromycin (10µg) $375
ZX110 pEZX-LvFL03 tdTomato reporter cloning vector (lentiviral) N/A tdTomato N/A Puromycin (10µg) $375

* A separate vector is available for SEAP expression

 

Secrete-Pair Luminescence Assay Kits

Using GLuc and/or SEAP as reporters? Then you will need a compatible assay kit.  The table below displays both single (GLuc alone) and dual (GLuc + SEAP) luminescence assay kits—exclusively from GeneCopoeia.

Buy Catalog# Product Description Price
LF031 Secrete-Pair Dual Luminescence Assay Kit Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF032 Secrete-Pair Dual Luminescence Assay Kit Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF033 Secrete-Pair Dual Luminescence Assay Kit Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF061 Secrete-Pair Gaussia Luciferase Assay Kit Detects Gaussia luciferase (GLuc) inquire
LF062 Secrete-Pair Gaussia Luciferase Assay Kit Detects Gaussia luciferase (GLuc) inquire