Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of about 39,500 human, 28,700 mouse and 17,500 rat promoters using live cell assays.GLuc-ON promoter clones can be ordered as pre-designed or custom-built in one of our robust vector systems. Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses GLuc, mCherry, or GFP as the promoter reporter. The dual-reporter system uses GLuc as the promoter reporter and SEAP (secreted Alkaline Phosphatase) as the internal control for signal normalization. GLuc and SEAP-containing promoter-reporter clones are fully compatible with GeneCopoeia’s Secrete-Pair™ dual luminescence assays kits.
Advantages
- Live cell assays. Naturally secreted GLuc and SEAP reporters makes lysis of the cells unnecessary. Reduces the number of samples required and sample-to-sample variation for applications such as pulse chase analysis and time-courses.
- Dual secreted reporter system. SEAP enables transfection normalization for accurate across-sample comparison
- High sensitivity. GLuc is 1,000-fold more sensitive than firefly and Renilla luciferases
- Convenience. All promoter clones are transfection-ready
Order pre-designed promoter clones
To order pre-designed promoter clones, search almost 40,000 human, 30,000 mouse, and 18,000 rat clones in your choice of vector.
Custom promoter clone services
Don’t see what you need in our collection of pre-designed promoter clones? Contact us for our custom services. Please complete our Custom Promoter Clone Inquiry and Quotation form, email inquiry@genecopoeia.com, or call: 866-360-9531.
Promoter lentivirus
GeneCopoeia also offers ready-to-use lentiviral particles expressing promoter-reporters as a custom service.
Vector choices
GLuc-ON™ promoter-reporter clones are available in a variety of vector types, which enable you to choose either the type of reporter, (GLuc, eGFP, etc.), whether or not you want a tracking gene for normalization, selection marker, and non-viral or lentiviral. Refer to the table below to discover the distinguishing features of Gluc-ON™ promoter-reporter vectors.
| Vector | Reporter gene | Tracking gene | Selection marker | Vector type |
|---|---|---|---|---|
| pEZX-PG04** | Gaussia luciferase (GLuc) | Secreted alkaline phosphatase (SEAP) | Puromycin | Non-viral |
| pEZX-PG02** | Gaussia luciferase (GLuc) | N/A* | Puromycin | Non-viral |
| pEZX-PF02 | eGFP | N/A* | Puromycin | Non-viral |
| pEZX-PM02 | mCherry | N/A* | Puromycin | Non-viral |
| pEZX-LvPG04** | Gaussia luciferase (GLuc) | Secreted alkaline phosphatase (SEAP) | Puromycin | HIV Lentiviral |
| pEZX-LvPG02** | Gaussia luciferase (GLuc) | N/A* | Puromycin | HIV Lentiviral |
| pEZX-LvPF02 | eGFP | N/A* | Puromycin | HIV Lentiviral |
| pEZX-LvPM02 | mCherry | N/A* | Puromycin | HIV Lentiviral |
| pEZX-LvPM03 | tdTomato | N/A* | Puromycin | HIV Lentiviral |
* A separate vector is available for SEAP expression.
** These Gaussia luciferase cloning vectors are available for purchase.
Control clones and SEAP expression clone
For most experiments with promoter-reporter clones, GeneCopoeia recommends including both positive and negative controls. See below to choose from a larger number of control clones. You can also purchase an SEAP expression clone, to normalize signals for differences in transfection efficiency for single reporter vectors.
