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HDR Donor Clones and Cloning Vectors

For convenient selection and flexible applications

Introduction

Homology-directed repair (HDR) is a natural DNA repair mechanism that is induced by DNA double-strand breaks (DSBs). Site-specific TALENs or CRISPR Cas9-sgRNA complexes generate DSBs in the chromosome, leading to DNA repair. In the presence of donor DNA, HDR occurs and replaces genetic information in the chromosome with new information from the donor gene.
Donor-mediated HDR makes many applications possible, such as precisely defined gene knockout, in-frame fusion tagging, transgene insertion, and introduction of base pair changes for mutagenesis or disease correction (Figure 1).

 

 

 

Figure 1. CRISPR-Cas9-based gene engineering. A reporter gene and a selection marker are integrated into the targeted genome site via DSB-induced HDR from a donor plasmid.