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COVID-19 Coronavirus Stable Cell Lines

General information

GeneCopoeia has produced HEK293T cell lines expressing ACE2 and TMPRSS2, two human host factors needed for efficient infection by SARS-CoV-2, the virus responsbile for the COVID-19 pandemic. These cell lines are great for use with either native SARS-CoV-2 virus or SARS-CoV-2 Spike-pseudotyped lentiviruses for several applications, such as:

  • Vaccine development for prevention of infection by SARS-CoV-2 virus
  • Studying the efficacy and mechanism of neutralizing antibodies against SARS-CoV-2 virus
  • Development of antiviral therapeutic agents
  • Studying the mechanism of virus-receptor interaction

 

In addition, we provide labeled cancer cell lines, which include 5 lung cancer cell line derivatives. The labeled lung cancer cell lines carry stable integration of either a combination of Firefly luciferase and GFP together, or GFP alone, and are great for drug target identification and compound screening either in vitro or in vivo.

You can get more information and order these cell lines using the drop-down menus below.

HEK293T cell lines expressing ACE2 and TMPRSS2

GeneCopoeia has produced HEK293T cell lines expressing human ACE2 alone, or ACE2 in combination with TMPRSS2, a protease that promotes Spike-ACE2 receptor interaction. These cell lines express ACE2 and/or TMPRRSS2 mRNA at high levels (Figure 1), and are amenable to infection by viruses expressing SARS-CoV-2 Spike protein in their envelopes (Figure 2).

You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
SL221 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2), 2×106 cells Embryonic kidney Hygromycin $1395 $3995
SL222 HEK293T HEK293T cells stably expressing human ACE2 (NM_021804.2) and TMPRSS2 (NM_005656.3), 2×106 cells Embryonic kidney

mCherry/

Puromycin, 

Hygromycin

$1795 $4795

 

  Warranty statement and limited use license   MSDS
 

 

Performance data

SL221 and SL222 are cell lines derived from HEK293T that express ACE2 alone or ACE2 and TMPRSS2 mRNA at high levels, as shown in Figure 1.

Figure 1. Relative mRNA expression levels of ACE2 and TMPRSS2 in GeneCopoeia stable cell lines. mRNA was amplified via RT-qPCR using primers specific for each gene. Ct values of ACE2 and TMPRSS2 were normalized to Ct values for housekeeping gene GAPDH. Relative expression levels were calculated from 2^ΔΔCt. A. ACE2 mRNA expression in parental cell line HEK293T and ACE2-overexpressing HEK293T cell line SL221. B. ACE2 and TMPRSS2 mRNA expression in parental cell line HEK293T and ACE2- and TMPRSS2-overexpressing cell line SL222.

GeneCopoeia's R&D team determined the ability of SARS-CoV-2 Spike-Pseudotyped Lentiviruses to infect ACE2-expressing cells, as shown in Figure 2.

Figure 2. ACE2- or ACE2/TMPRSS2-expressing HEK293T cells transduced by eGFP/hLuc-expressing SARS-CoV-2 full length wildtype (D614) spike pseudotyped lentivirus (pseudovirus). 10 μl of pseudovirus (Cat.No. SP101-100) was used to transduce each cell line in a 96-well plate. 72 hours after the transduction, cells were photographed under fluorescence microsopy and luciferase activities were measured. A. ACE2/TMPRSS2-expressing HEK293T stable cell line (cat.No. SL222) B. ACE2-expressing HEK293T stable cell line (cat.No. SL221) C. HEK293T only cell line. D. 20 μl of cell lysate from pseudovirus transduced cell cultures or the negative control cell culture (NC) was analyzed for firefly luciferase activity using GeneCopoeia's Luc-Pair™ Duo-Luciferase HS Assay Kit (cat.No. LF007).

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.

 

Labeled stable lung cancer cell lines

GeneCopoeia carries lung cancer cell lines, which contain both firefly luciferase and GFP, or GFP alone, integrated into the genome. These are valuable for COVID-19 coronavirus research since the SARS-CoV-2 virus enters the body via lung epithelial cells. These cell lines, which part of GeneCopoeia's extensive collection of labeled cancer cell lines, are great for drug target identification and compound screening either in vitro or in vivo.

You can select your cell line of interest from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.

Buy Catalog # Cell line Description Cell type Reporter genes Price, Academic Price, Industry
SL001 NCI-H1299 NCI-H1299 human lung non-small cell carcinoma cancer cell line dual-labeled with luciferase and GFP Lung cancer Luciferase GFP $945 $1945
SL002 NCI-H661 NCI-H661 human lung large cell carcinoma cancer cell line dual-labeled with luciferase and GFP Lung cancer Luciferase GFP $945 $1945
SL003 NCI-H1975 NCI-H1975 human lung non-small cell adenocarcinoma cancer cell line dual-labeled with luciferase and GFP Lung cancer Luciferase GFP $945 $1945
SL026 HCC827 HCC827 human lung adenocarcinoma cancer cell line dual-labeled with luciferase and GFP Lung cancer Luciferase GFP $945 $1945
SL028 H1437 H1437 human lung stage 1 adenocarcinoma cancer cell line dual-labeled with luciferase and GFP Lung cancer Luciferase GFP $945 $1945
SL101 NCI-H1299 NCI-H1299 human lung non-small cell carcinoma cancer cell line labeled with GFP Lung cancer GFP $945 $1945
SL102 NCI-H661 NCI-H661 human lung large cell carcinoma cancer cell line labeled with GFP Lung cancer GFP $945 $1945
SL103 NCI-H1975 NCI-H1975 human lung non-small cell adenocarcinoma cancer cell line labeled with GFP Lung cancer GFP $945 $1945

 

  Warranty statement and limited use license   MSDS
 

 

 

 

For more information or to request a quote, please contact inquiry@genecopoeia.com.