GeneCopoeia has produced HEK293T cell lines expressing ACE2 and TMPRSS2, two human host factors needed for efficient infection by SARS-CoV-2, the virus responsible for the COVID-19 pandemic.
These cell lines express ACE2 and/or TMPRSS2 mRNA at high levels on the cell surface, and are amenable to infection by viruses expressing SARS-CoV-2 Spike protein in their envelopes. Refer to the "Performance data" tab for more information.
ACE2-expressing cell lines are great for use with either native SARS-CoV-2 virus or SARS-CoV-2 Spike-pseudotyped lentiviruses for several applications, such as:
- Vaccine development for prevention of infection by SARS-CoV-2 virus
- Studying the efficacy and mechanism of neutralizing antibodies against SARS-CoV-2 virus
- Development of antiviral therapeutic agents
- Studying the mechanism of virus-receptor interaction
You can order these cell lines from the table below. To obtain the datasheet for each cell line, please click on the designated catalog number.
In addition to the products below, custom stable cell lines are also available. For more information, please contact inquiry@genecopoeia.com.
For more information or to request a quote, please contact inquiry@genecopoeia.com.
Performance data
SL221 and SL222 are cell lines derived from HEK293T that express ACE2 alone or ACE2 and TMPRSS2 mRNA at high levels, as shown in Figure 1. Expression of ACE2 protein (Figure 2) and/or TMPRSS2 protein (Figure 3) on the cell surface makes these cells amenable to infection by Spike-pseudotyped lentivirus (Figure 4).
Figure 1. Relative mRNA expression levels of ACE2 and TMPRSS2 in GeneCopoeia stable cell lines. mRNA was amplified via RT-qPCR using primers specific for each gene. Ct values of ACE2 and TMPRSS2 were normalized to Ct values for housekeeping gene GAPDH. Relative expression levels were calculated from 2^ΔΔCt. A. ACE2 mRNA expression in parental cell line HEK293T and ACE2-overexpressing HEK293T cell line SL221. B. ACE2 and TMPRSS2 mRNA expression in parental cell line HEK293T and ACE2- and TMPRSS2-overexpressing cell line SL222.
Figure 2. Immunofluorescence (IF) analysis of ACE2 protein expression on the cell surface of ACE2-expressing HEK293T cells (Cat.# SL221), ACE2/TMPRSS2 expressing HEK293T cells (Cat.# SL222), and HEK293T cells. Unpermeablized cells were fixed, blocked and incubated with the primary anti-ACE2 antibody, then washed and incubated with fluorescent-labeled secondary antibody, washed again, and stained with DAPI.
Figure 3. Immunofluorescence (IF) analysis of TMPRSS2 protein expression on the cell surface of TMPRSS2-expressing HEK293T cells (Cat.# SL222), and HEK293T cells. Unpermeablized cells were fixed, blocked and incubated with the primary anti-TMPRSS2 antibody, then washed and incubated with fluorescent-labeled secondary antibody, washed again, and stained with DAPI.
GeneCopoeia's R&D team determined the ability of SARS-CoV-2 Spike-Pseudotyped Lentiviruses to infect ACE2-expressing cells, as shown in Figure 4.
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Figure 4. ACE2- or ACE2/TMPRSS2-expressing HEK293T cells transduced by eGFP/hLuc-expressing SARS-CoV-2 Spike pseudotyped lentiviruses (pseudoviruses). 10 μl of pseudovirus was used to transduce each cell line in a 96-well plate. 72 hours after transduction, cells were photographed under fluorescence microscopy, followed by measurement of luciferase activities or FACS sorting. SL221: HEK293T cells expressing ACE2. SL222: HEK293T cells expressing ACE2 and TMPRSS2. SP101: Full-length Spike protein, D614 variant. SP103: Full-length Spike protein, D614G variant. SP001: Truncated Spike protein, D614 variant. SP003: Truncated Spike protein, D614G variant. A. Fluorescence microscopy. B. Luciferase activity assay. 20 μl of cell lysate from pseudovirus transduced cell cultures were analyzed for firefly luciferase activity using GeneCopoeia's Luc-Pair™ Firefly Luciferase HS Assay Kit (cat. no. LF007). C. FACS sorting for determination of titer in transduction units/ml.
Webinars
Title: Advanced Tools For COVID-19 Coronavirus Research From GeneCopoeia
Presented Wednesday, September 9, 2020
The COVID-19 global pandemic, caused by the SARS-CoV-2 coronavirus, highlights an urgent need for diagnostic reagents, antiviral therapeutics, and vaccine development. In this webinar, we will discuss GeneCopoeia's advanced solutions including testing kits, SARS-CoV-2 pseudotyped virus, and other tools needed for research into combating this deadly disease.
Watch recorded webinar / Download slides
Publications
View selected publications citing GeneCopoeia's ACE2 receptor cell lines from the recent literature
2023
- Kuroda, T., et al. (2023). Efficacy comparison of 3CL protease inhibitors ensitrelvir and nirmatrelvir against SARS-CoV-2 in vitro and in vivo. J Antimicrob Chemother doi: 10.1093/jac/dkad027 [ACE2 and TMPRSS2-expressing HEK293T cells]
- Osaka, T., et al. (2023). Cross-Reactivity of Antibodies in Intravenous Immunoglobulin Preparation for Protection against SARS-CoV-2. Microorganisms doi: 10.3390/microorganisms11020471 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
- Chan, J.F-K., et al. (2023). Altered host protease determinants for SARS-CoV-2 Omicron. Sci Adv doi: 10.1126/sciadv.add3867 [ACE2-expressing HEK293T stable cell line]
- Merluza, J., et al. (2023). Validation and Establishment of the SARS-CoV-2 Lentivirus Surrogate Neutralization Assay as a Prescreening Tool for the Plaque Reduction Neutralization Test. Microbiol Spectr doi: 10.1128/spectrum.03789-22 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
- Nakata, Y., et al. (2023). Cellular APOBEC3A deaminase drives mutations in the SARS-CoV-2 genome. Nucleic Acids Res doi: 10.1093/nar/gkac1238 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
2022
- Sun, P.F., et al. (2022). Asymptomatic or symptomatic SARS-CoV-2 infection plus vaccination confers increased adaptive immunity to variants of concern. iScience doi: 10.1016/j.isci.2022.105202 [HEK293-ACE2 expressing cell lines]
- Suzuki, Y., et al. (2022). Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primates. Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2022.09.017 [ACE2 and TMPRSS2-expressing HEK293T cells]
- Hwang, J-Y., et al. (2022). Humoral and Cellular Responses to COVID-19 Vaccines in SARS-CoV-2 Infection-Naïve and -Recovered Korean Individuals. Vaccines doi: 10.3390/vaccines10020332 [ACE2-expressing HEK293T stable cell line]
- Park, H., et al. (2022). TMED3 Complex Mediates ER Stress-Associated Secretionof CFTR, Pendrin, and SARS-CoV-2 Spike. Advanced Science doi: 10.1002/advs.202105320 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
- Sim, J-R., et al. (2022). Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition. Cell Reports doi: 10.1016/j.celrep.2022.111117 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
- Grodzki, M., et al. (2022). Genome-scale CRISPR screens identify host factors that promote human coronavirus infection. Genome Medicine doi: 10.1186/s13073-022-01013-1 [ACE2-expressing HEK293T stable cell line]
- Ariumi, Y. (2022). Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection. Journal of Virology doi: 10.1128/jvi.00002 [ACE2-expressing HEK293T stable cell line]
- Pivniouk, V., et al. (2022). The OM-85 bacterial lysate inhibits SARS-CoV-2 infection of epithelial cells by downregulating SARS-CoV-2 receptor expression. Journal of Allergy and Clinical Immunology doi: 10.1016/j.jaci.2021.11.019 [ACE2-expressing HEK293T stable cell line]
- Travis, B.J., et al. (2022). Significance of chlorine-dioxide-based oral rinses in preventing SARS-CoV-2 cell entry. Oral Diseases doi: 10.1111/odi.14319 [ACE2-expressing HEK293T stable cell line]
2021
- Hashizume, M., et al. (2021). Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro. Viruses doi: 10.3390/v13010067 [ACE2-expressing HEK293T stable cell line]
- Ao, Z., et al .(2021). Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry. PLOS ONE doi: 10.1371/journal.pone.0251649 [ACE2-expressing HEK293T stable cell line]
- Paidi, R.K., et al. (2021). Eugenol, a Component of Holy Basil (Tulsi) and Common Spice Clove, Inhibits the Interaction Between SARS-CoV-2 Spike S1 and ACE2 to Induce Therapeutic Responses. Journal of Neuroimmune Pharmacology doi: 10.1007/s11481-021-10028-1 [ACE2-expressing HEK293T stable cell line]
- Paidi, R.K., et al. (2021). Selective Inhibition of the Interaction between SARS-CoV-2 Spike S1 and ACE2 by SPIDAR Peptide Induces Anti-Inflammatory Therapeutic Responses. Journal of Immunology doi: 10.4049/jimmunol.2100144 [ACE2-expressing HEK293T stable cell line]
2020
- Reznikov, L.R., et al. (2020). Identification of antiviral antihistamines for COVID-19 repurposing. Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2020.11.095 [ACE2-expressing HEK293T stable cell line]