GeneCopoeia SuperCut™ Nuclease is a genetically engineered endonuclease derived from Serratia marcescens. Structurally, the protein is a dimer containing 245 amino acids carrying 2 key disulfide bonds and 30 kD subunits. The enzyme degrades all forms of DNA and RNA (single-stranded, double-stranded, linear or circular) and is effective over a wide range of conditions. The enzyme completely digests 2 to 5 bases in oligonucleotides from the nucleic acid to the 5′-phosphate terminus. Although the nuclease can digest almost all positions of the nucleotide chain, it is still somewhat sequence specific, preferring the GC-rich regions of double-stranded DNA and avoiding AT-rich regions. With its highly specific activity and absence of protease activity and viral contaminants, this product is an ideal and widely used nuclease for the complete digestion of nucleic acids. Enzyme activity: 250 U/μL Source: Serratia Marcescens Host: E.Coli Storage buffer and condition:10 mM Tris-HCl, 20 mM NaCl, 2 mM MgCl2, 50% Glycerol (pH 8.0). Store at -20℃ and stable for at least 12 months. Unit definition: One unit is defined as the amount of enzyme that causes a ∆A260 of 1.0 in 30min. Purity: ≥ 95%, as determined by SDS-PAGE
Application
- Removal of nucleic acids from recombinant proteins
- Viscosity reduction during cell lysis preparation
- Sample preparation for 2D gel electrophoresis and SDS-PAGE electrophoresis
- Removal of nucleic acid during vaccine and viral vector preparation
- Decrease or prevent cell aggregation during PBMC thawing and organoid processing
- Improve the quality of ELISA, Western Blot, protein chip and mass spectrometry analysis results during blood sample processing
Performance data
| Remove nucleic acids | Reduce the viscosity of cell lysates |
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| E.coli BL21(DE3) cells containing one pET were suspended in GeneCopoeia reagent (5 mL/g wet weight). Suspension samples were treated with SuperCut™ Nuclease for 30min at room temperature, and agarose gel electrophoresis was performed after centrifugation to clarify the samples. | E.coli BL21(DE3) cells containing one pET were suspended in GeneCopoeia reagent (5 mL/g wet weight). Suspension samples were treated with SuperCut™ Nuclease for 10min at room temperature. Photographs were taken after centrifugation at 377 × g for 3 min. |