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Allergen Antigen Array

GeneCopoeia’s OmicsArray™ Common Allergens Antigen Microarray is a protein microarray enabling powerful detection of antibodies associated with allergies. The array carries 75 superior-quality purified antigens spotted onto nitrocellulose filters, which are adhered to glass slides. Antigens known to be associated with specific allergy triggering disorders are chosen based on a thorough review of peer-reviewed publications.

In addition to this premade array, arrays containing customized sets of antigens are available, as well as array profiling services and data analysis. To order premade or custom arrays, please contact us.

GeneCopoeia’s OmicsArray™ Allergen Antigen Array is part of the GeneCopoeia OmicsArray™ Antigen Microarray family.

Return to the main OmicsArray™ Antigen Microarray page

 

Advantages of OmicsArray™ Antigen Microarrays
  • Largest collection of pre-made whole-protein antigen microarrays on the market.
  • Largest number of whole-protein antigens specifically focused on autoimmune diseae research.
  • Best combination of number of antigens per array (up to 120) with number of samples that can be processed per slide (up to 15).

 

OmicsArray™ premade antigen arrays

For pricing, please inquire.

Cat. No. Description Application Antigen list
PA006 Allergen Antigen Array Profiling antibodies (IgG, IgE) reacting with common allergens Download

 

 

 

In addition to premade arrays, arrays containing customized sets of antigens are available, as well as array processing kits, array profiling services and data analysis.

 

Antigen microarray processing kits

GeneCopoeia's OmicsArray™ Antigen Microarray Processing Kits for human and mouse contain all the reagents needed for users with a compatible microarray scanner (not included) to incubate samples (serum, plasma, cerebrospinal fluid. and other body fluids) with OmicsArray™ Antigen Microarrays: Hybridization and washing buffers, DNase I, DTT, and anti-human or anti-mouse labeled secondary antibodies (Cy3-IgG, Cy5-IgM). Slide trays and gaskets sold separately.

Prices are for customers from the U.S. and Canada only. For other customers, please contact your local distributor for details.

Buy Catalog number Description Price
PA100 OmicsArray™ Antigen Microarray Processing Kit, Human (for processing up to 4 slides) $250
PA101 OmicsArray™ Antigen Microarray Processing Kit, Mouse (for processing up to 4 slides) $250
PA102 OmicsArray™ 4-slide Tray and Gasket Set $325
PA103 OmicsArray™ 16-well Slide Gasket (pack of 2) $100

 

User Manual:

 

SDS:

Custom services

GeneCopoeia offers custom antigen microarray services in the following areas:

  • Custom array printing. GeneCopoeia will create custom antigen microarrays built to your specifications.
  • Sample processing. Send us your blood, plasma, tissue, or other biological sample and we will prepare it for processing and incubation with any of our premade antigen microarrays or custom-built antigen microarrays for autoantibody profiling and other applications. For information on sample types to submit, consult the FAQ
  • Data analysis. Once samples are processed and incubated with an antigen microarray, we will analyze the raw data. The standard analysis service includes: 1) An Excel file of the Net Signal Intensity (NSI) for each antigen on the array, normalized to internal controls; and 2) a heat map

Additional analysis services, including proteomic analysis, pathway analysis, and more, are also available.

To inquire about custom antigen microarray products and services, please fill out our custom quote request form

 

Technology overview

Technology overview

GeneCopoeia's OmicsArray™ antigen microarrays contain up to 120 purified proteins spotted onto nitrocellulose filters, which are adhered to glass slides. In addition, 8 spots are included for normalization. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control. As little as 1 ul serum or 50 ul of other bio fluids are needed for each sample.

As shown in Figure 1, arrays are incubated with patient samples, and any antibodies in the  samples bind to their cognate antigens on the array. The arrays are washed to remove unbound antibodies and other proteins, then co-incubated with Cy3- and Cy5-labeled secondary antibodies. The dual labeling strategy is intended to distinguish between immunoglobulin (Ig) subtypes present within samples. For example. a Cy3-labeled anti-IgG secondary antibody is used to detect IgG antibodies, and a Cy5-labeled anti-IgM secondary antibody is used to detect IgM antibodies. Fluorophore-labeled secondary antobodies are available for detecting IgA, IgD, IgE, IgG and IgM immunoglubulins, as well as IgG subclasses IgG1, IgG2, IgG3, and IgG4.

After washing to remove unbound secondary antibodies, signals are detected using a GenePix® 4400A microarray scanner. The raw data is then analyzed using GenePix® 7.0 software.

 

Figure 1. Workflow for detection of antibodies in samples using GeneCopoeia's OmicsArray™ antigen microarrays.

Return to the main OmicsArray™ Antigen Microarray page