GeneCopoeia’s GLuc-ON™ LXRa transcriptional response element (TRE) clone enables analysis of the transcriptional activity of liver X receptor (LXR). LXRs comprise 2 members: LXRa and LXRb. LXRs are nucleus-localized transcription factors that bind oxidized forms of cholesterol, and are critical players in regulation of cholesterol levels.
The GLuc-ON™ LXRa TRE clone contains tandem repeats of LXRa upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The LXRa response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ LXRa TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ LXRa TRE by exogenous expression of LXRa. 293T cells were were co-transfected with (left to right) negative TRE clone + negative expression clone, negative TRE clone + LXRa expression clone, LXRa TRE clone + negative expression clone, or LXRa TRE clone + LXRa expression clone. Transfected cells were incubated for 40 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.