GeneCopoeia's OncoSpot™ NGS Reference Standards use highly characterized HCT116 cell lines, genetically modified with advanced genome editing technology to include clinically relevant cancer gene mutations, just as they occur in patient tumors. The engineered mutations represent SNVs and deletions; the cell lines are manufactured in an ISO9001 certified facility to ensure lot-to-lot stability and reproducibility.
The reference standards are in the formats of genomic DNA and formalin-fixed paraffin-embedded (FFPE). They are compatible with multiple genomic platforms, e.g. NGS, sanger sequencing and qPCR, and are ideal to determine the performance criteria of the workflows, including:
- DNA isolation
- NGS library construction
- Sample preparation
- Sequencing
The reference standards are ideal for NGS assay development, quality control and validation for detection limit, precision and accuracy.