Product Information DNase I is an endonuclease that digests single- and double- stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5’-phosphate and 3’-OH groups. The enzyme activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ or Mn²⁺ ions. If in the presence of Mg²⁺, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion. If in the presence of Mn²⁺, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with one or two nucleotide overhangs.

Applications

• Preparation of DNA-free RNA samples
• Removal of DNA contamination from RNA samples prior to RT-qPCR reaction
• DNA template after removal of RNA transcription in vitro
• DNase I footprinting assay
• Construction of DNA random fragment library