Introduction
GeneCopoeia’s GLuc-ON™ JNK transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins, and is necessary for signaling through the Stress-activated protein kinases (SAPK)/Jun amino-terminal kinases (JNK) (SAPK/JNK) pathway.
The GLuc-ON™ JNK TRE clone contains tandem repeats of an AP1 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The number of AP1 response elements has been optimized to maximize the sensitivity and minimize background levels (Figure 1). The GLuc-ON™ JNK TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ JNK TRE by treatment with phorbol 12-myristate 13-acetate (PMA). HEK293a cells were transfected with a negative control clone or the GLuc-ON™ JNK TRE clone. Transfected cells were incubated for 24 hours, then treated with different amounts of PMA for 24 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation.