GeneCopoeia’s GLuc-ON™ CREB transcriptional response element (TRE) lentiviral clone enables analysis of the effects on the cAMP and PKA signaling pathways through the cAMP response element (CRE). CRE, which is found in the promoters of many genes, including somatotatin, vegetative growth factor (VGF), and the protooncogene c-fos,becomes bound by the transcription factor CRE binding protein (CREB) in response to elevated cAMP levels.
The GLuc-ON™ CREB TRE lentiviral clone contains tandem repeats of CRE upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The CREB response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ CREB TRE lentiviral clone is provided as transfection-ready DNA. A negative (non-activating) control lentiviral clone is also available for purchase.
Figure 1. Activation of the GLuc-ON™ CREB TRE by forskolin. HEK293a cells were transduced with negative control lentiviral partices or the GLuc-ON™ CREB TRE lentiviral particles. Transduced cells were incubated for 48 hours, followed by 6 days incubation in the presence of puromycin. Puromycin-resistant cells were then treated with forskolin for 18 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.