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GeneHero™ CRISPR Products and Services

GeneCopoeia's GeneHero™ CRISPR-Cas9 products and services provide a complete, powerful solution to your genome editing needs. Products and services include:

  • CRISPR Plasmids

Transfect cells with our CRISPR plasmids with Cas9 and sgRNA for human, mouse, and rat. Search our database of more than 45,000 human, mouse, and rat genes for genome editing using CRISPR. 

  • CRISPR Lentivirus

Genome integration of CRISPR elements using lentivirus. Cas9 and/or sgRNA packed in purified lentiviral particles at 108TU/ml, ready to infect all cell types.

  • CRISPR AAV

Episomal expression of CRISPR components with adeno-associated viral particles carrying Cas9 and/or sgRNA, excellent for tissue and animal transduction.

Premade Cas9-expressing stable cell lines are great for sgRNA library screening and other high-throughput CRISPR-Cas9 applications.

Advantages

  • RNA-guided genomic DNA recognition regardless of the methylation status
  • Similar or greater gene-editing efficiency compared to ZFNs and TALENs
  • Capable of editing multiple genes simultaneously (multiplexing)
  • Simple and fast design process. No need to reengineer the nuclease for each new target

Product Information

CRISPR Plasmids

sgRNA expression plasmids are available for targeting virtually any gene in any experimental system. sgRNA plasmids express either sgRNA only, or sgRNA + Cas9 nuclease in an all-in-one format. Lentiviral clones express sgRNA alone. Cas9-expressing lentiviral clones are available separately.

The CRISPR plasmid options listed below are intended for using 20 ntsgRNAs with the wild-type Cas9 nuclease from Streptococcus pyogenes(SpCas9). GeneCopoeia also provides alternate CRISPR options, such as Cas9 D10A nickase and other high-fidelity versions of Cas9, SaCas9, and shorter sgRNAs. To purchase reagents for these other options, please contact us for a custom quote.

Custom sgRNA Expression Plasmids

Vector Promoter sgRNA and Cas9 Selection Marker/ Reporter Gene Vector Type Vector Map
All-in-one (Cas9 nuclease + sgRNA) clones
pCRISPR-CG02 U6 sgRNA and CBh-driven Cas9 (wild type) in the same vector N/A Non-viral get_info
pCRISPR-CG04 U6 sgRNA and CMV-driven Cas9 (wild type) in the same vector Neomycin / copGFP Non-viral get_info
pCRISPR-CG07 U6 sgRNA and CBh-driven Cas9 (wild type) in the same vector copGFP Non-viral get_info
pCRISPR-CG08 U6 sgRNA and CBh-driven Cas9 (wild type) in the same vector mCherry Non-viral get_info
pCRISPR-CG12 U6 sgRNA and CMV-driven Cas9 (wild type) in the same vector Neomycin / mCherry Non-viral get_info
sgRNA only clones
pCRISPR-SG01 U6 sgRNA only Hygromycin Non-viral get_info
pCRISPR-LvSG03 U6 sgRNA only Puromycin / mCherry Lentiviral get_info

Cas9 Nuclease Expression Plasmids

Clones expressing human codon-optimized S. pyogenes Cas9 nuclease or D10A nickase are available. These clones do not express sgRNAs.

Buy Catalog# Product Promoter Reporter gene / Selection marker Price
Cas9 nuclease and D10A nickase expression clones
CP-C9NU-01 Cas9 nuclease expression clone CMV mCherry / Neomycin $595*
CP-C9NI-01 Cas9 D10A nickase expression clone CBh N/A $595*
CP-C9NI-02 Cas9 D10A nickase expression clone CMV mCherry / Neomycin $595*
Cas9 nuclease lentiviral expression clones
CP-LvC9NU-01 Cas9 nuclease lentiviral expression clone CMV Neomycin $595*
CP-LvC9NU-02 Cas9 nuclease lentiviral expression clone CMV eGFP / Neomycin $595*
CP-LvC9NU-08 Cas9 nuclease lentiviral expression clone EF1a Puromycin $595*
CP-LvC9NU-09 Cas9 nuclease lentiviral expression clone EF1a eGFP/Neomycin $595*
CP-LvC9NU-10 Cas9 nuclease lentiviral expression clone EF1a eGFP/Hygromycin $595*

 

 * Discount applied when purchased together with a sgRNA clone.  

Click here to search our database of sgRNA plasmids for knocking out more than 45,000 human, mouse, and rat genes.

CRISPR Lentivirus

Custom sgRNA Lentiviral Particles

sgRNA for human, mouse, and rat packaged in high-titer lentivirus.

Vector Promoter sgRNA and Cas9 Selection Marker/ Reporter Gene Vector Type Vector Map
pCRISPR-LvSG03 U6 sgRNA only Puromycin / mCherry Lentiviral get_info

Pre-made sgRNA Control Particles

Buy Catalog# Product Description Vector Price
Pre-made purified sgRNA control lentiviral particles
LPPCCPCTR01L03-025 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 25 µL (U6/T7/mCherry/Puro) pCRISPR-LvSG03 $295
LPPCCPCTR01L03-100 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 25 µL (U6/T7/mCherry/Puro) pCRISPR-LvSG03 $725

 

 

Pre-made Cas9 Nuclease Lentiviral Particles

Buy Catalog Product Name Description Price
LPP-CP-LvC9NU-01-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) >1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (CMV/Neomycin) inquire
LPP-CP-LvC9NU-02-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (CMV/eGFP/Neomycin) inquire
LPP-CP-LvC9NU-08-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/Puromycin) inquire
LPP-CP-LvC9NU-09-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/eGFP/Neomycin) inquire
LPP-CP-LvC9NU-10-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/eGFP/Hygromycin) inquire

CRISPR AAV

sgRNA and Cas9 AAV Particles

Vector name CRISPR Description Reporter gene
pCRISPR-AD01 SaCas9 & sgRNA All-in-one AAV vector with SaCas9 and sgRNA N/A
pCRISPR-AV20 sgRNA only AAV vector expressing sgRNA driven by U6 promoter N/A

Cas9 Stable Cell Lines

 

Pre-made Cas9 Stable Cell Lines

Visit the Cas9 stable cell line page for a full list of the Cas9 cell line collection.

GeneCopoeia also offers stable cell lines constitutively expressing the CRISPR Cas9 nuclease, enabling you to carry out CRISPR genome editing applications with high efficiency. The GeneHero™ Cas9 stable cell lines are available pre-made in many human cell lines, and in mouse cell line Neuro2a. The CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites (Figure 1), or randomly via lentiviral-mediated transduction.

Buy Catalog # Cell line Description Cell type Selection marker Price, Academic Price, Industry
Human cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL502 HEK293T Human cell line HEK293T stably expressing Cas9 from AAVS1 site Embryonic kidney Puro $945 $2695
SL554 HEK293 Human cell line HEK293 stably expressing Cas9 from AAVS1 site, with loxP Embryonic kidney Hygro $945 $2695
SL501 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from AAVS1 site Lung cancer Puro $1395 $3995
SL533 NCI-H1299 Human cell line NCI-H1299 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL504 A549 Human cell line A549 stably expressing Cas9 from AAVS1 site Lung cancer Hygro $1395 $3995
SL529 H1975 Human cell line H1975 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL536 NCI-H1437 Human cell line NCI-H1437 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL561 NCI-H661 Human cell line NCI-H661 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL578 HCC827 Human cell line HCC827 stably expressing Cas9 from random site Lung cancer Hygro $1395 $3995
SL503 HeLa Human cell line HeLa stably expressing Cas9 from AAVS1 site Cervical cancer Hygro $1395 $3995
SL514 MCF-7 Human cell line MCF-7 stably expressing Cas9 from AAVS1 site Breast cancer Hygro $1395 $3995
SL524 MCF-7 Human cell line MCF-7 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL515 MDA-MB-231 Human cell line MDA-MB-231 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL516 MDA-MB-468 Human cell line MDA-MB-468 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL517 T47D Human cell line T47D stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL535 SK-BR-3 Human cell line SK-BR-3 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL544 HCC70 Human cell line HCC70 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL547 DU4475 Human cell line DU4475 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL558 HCC1428 Human cell line HCC1428 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL562 CAMA-1 Human cell line CAMA-1 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL563 HCC38 Human cell line HCC38 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL574 AU-565 Human cell line AU-565 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL575 HCC1500 Human cell line HCC1500 stably expressing Cas9 from random site Breast cancer Hygro $1395 $3995
SL522 SNU-475 Human cell line SNU-475 stably expressing Cas9 from random site (backorder) Liver cancer Hygro $1395 $3995
SL530 SNU-449 Human cell line SNU-449 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL531 PLC/PRF/5 Human cell line PLC/PRF/5 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL537 SNU-387 Human cell line SNU-387 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL538 SNU-423 Human cell line SNU-423 stably expressing Cas9 from random site Liver cancer Hygro $1395 $3995
SL543 C3A Human cell line C3A stably expressing Cas9 from random site (backorder) Liver cancer Hygro $1395 $3995
SL557 SK-HEP-1 Human cell line SK-HEP-1 stably expressing Cas9 from random site Liver/ascites cancer Hygro $1395 $3995
SL520 AGS Human cell line AGS stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL526 SNU-1 Human cell line SNU-1 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL527 KATOIII Human cell line KATOIII stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL545 SNU-16 Human cell line SNU-16 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL565 NCI-N87 Human cell line NCI-N87 stably expressing Cas9 from random site Stomach/gastric cancer Hygro $1395 $3995
SL521 BXPC-3 Human cell line BXPC-3 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL528 HPAF-II Human cell line HPAF-II stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL540 CFPAC-1 Human cell line CFPAC-1 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL570 Panc 10.05 Human cell line Panc 10.05 stably expressing Cas9 from random site Pancreatic cancer Hygro $1395 $3995
SL523 HT-29 Human cell line HT-29 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL525 LoVo Human cell line LoVo stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL539 RKO Human cell line RKO stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL541 T84 Human cell line T84 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL542 COLO 205 Human cell line COLO 205 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL551 SNU-C1 Human cell line SNU-C1 stably expressing Cas9 from random site Colon cancer Hygro $1395 $3995
SL572 HCT116 Human cell line HCT116 stably expressing Cas9 from AAV1 site Colon cancer Hygro $1395 $3995
SL532 LS411N Human cell line LS411N stably expressing Cas9 from random site Caecum cancer Hygro $1395 $3995
SL583 Granta-519 Human cell line Granta-519 stably expressing Cas9 from random site Blood cancer Hygro $1395 $3995
SL546 U-2-OS Human cell line U-2 OS stably expressing Cas9 from random site Bone cancer Hygro $1395 $3995
SL552 K562 Human cell line K562 stably expressing Cas9 from random site Bone marrow cancer Hygro $1395 $3995
SL555 Jurkat Human T lymphocyte cell line Jurkat stably expressing Cas9 from random site Acute T cell leukemia Hygro $1395 $3995
SL548 DU145 Human cell line DU145 stably expressing Cas9 from random site Prostate cancer Hygro $1395 $3995
SL550 SH-SY5Y Human cell line SH-SY5Y stably expressing Cas9 from random site Neuroblastoma Hygro $1395 $3995
SL560 T24 Human cell line T24 stably expressing Cas9 from random site Bladder cancer Hygro $1395 $3995
SL566 HK2 Human cell line HK2 stably expressing Cas9 from random site Immortalized kidney cell line Hygro $1395 $3995
SL577 786-O Human cell line 786-O stably expressing Cas9 from random site Kidney cancer Hygro $1395 $3995
SL576 1321N1 Human cell line 1321N1 stably expressing Cas9 from random site Brain astrocytoma Hygro $1395 $3995
SL584 U87-MG Human cell line U87-MG stably expressing Cas9 from AAVS1 site Brain glioma Hygro $1395 $3995
SL512 HT1080 Human cell line HT1080 stably expressing Cas9 from AAVS1 Fibrosarcoma Hygro $1395 $3995
SL580 SK-OV-3 Human cell line SK-OV-3 stably expressing Cas9 from random site Ovary Hygro $1395 $3995
Human cell lines stably expressing high-fidelity S. pyogenes Cas9 (SpCas9-HF1)
SL553 HEK293 Human cell line HEK293 stably expressing SpCas9-HF1 from AAVS1 site Embryonic kidney Hygro $1795 $4995
Mouse cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL509 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Hygro $1395 $3995
SL559 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site, with loxP Neuroblastoma Hygro $1395 $3995
SL510 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Puro $1395 $3995
SL511 Neuro2a Mouse cell line Neuro2a stably expressing Cas9 from ROSA26 site Neuroblastoma Neo $1395 $3995
SL564 C2C12 Mouse cell line C2C12 stably expressing Cas9 from random site Muscle (myoblast) Hygro $1395 $3995
SL567 BA/F3 Mouse cell line BA/F3 stably expressing Cas9 from random site Lymph Hygro $1395 $3995
SL569 NIH-3T3 Mouse cell line NIH-3T3 stably expressing Cas9 from random site Embryo fibroblast Hygro $1395 $3995
SL581 4T1 Mouse cell line 4T1 stably expressing Cas9 from random site Mammary gland Hygro $1395 $3995
SL582 CT26 Mouse cell line CT26 stably expressing Cas9 from random site Colon Hygro $1395 $3995
Mouse cell lines stably expressing high-fidelity S. pyogenes Cas9 (SpCas9-HF)
SL571 Neuro2a Mouse cell line Neuro2a stably expressing SpCas9-HF from ROSA26 site Neuroblastoma Hygro $1795 $4995
Rat cell lines stably expressing S. pyogenes Cas9 (SpCas9)
SL534 C6 Rat cell line C6 stably expressing Cas9 from random site Glioma Hygro $1395 $3995

  Warranty statement and limited use license   MSDS
 

 

Note: GeneCopoeia also offers custom-built CRISPR cell line services. To inquire, please contact inquiry@genecopoeia.com.