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miExpress™ Precursor miRNA Expression Clones

Introduction

MicroRNAs (miRNAs) are small, non-coding single stranded RNA molecules found in eukaryotic organisms. They are highly conserved and usually 21-23 nucleotides in length. miRNAs are involved in almost all cellular functions and regulate gene expression by binding to the 3' untranslated regions (3' UTRs) of targeted mRNAs. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

GeneCopoeia offers miExpress™ precursor miRNA expression clones in lentiviral and non-viral vectors with eGFP reporter gene. Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types. Clone sets are also available.

 

Advantages         

 Full coverage

  • All known human, mouse and rat miRNAs in the current miRBase covered.

Flexible delivery systems

  • Lentiviral vectors allow efficient transduction and stable integration into the host genome of non-dividing and difficult to transfect cells target cells
  • Non-viral vectors allow either transient or stable transfection

Optimized expression cassettes

  • Expression cassettes have been fully sequenced and optimized for high miRNA expression of precursor miRNA and maturation of miRNA inside cells

Selection markers and reporters

  • An eGFP reporter is designed to monitor transfection or transduction efficiency
  • Neomycin or Puromycin selection marker enables stable cell line selection
Figure 2: Role of precursor miRNA and miRNA 3' UTR target clones in miRNA regulation studies