Extractable Nuclear Antigen (ENA) and Antinuclear Antibody (ANA) Antigen Array

Product Information

Extractable Nuclear Antigens (ENA) and antinuclear antibodies (ANA) are crucial biomarkers that are used to indicate the presence of systemic autoimmune rheumatic diseases.

GeneCopoeia’s OmicsArray™ ENA and ANA 32-Plex Antigen Array is a highly sensitive protein microarray that offers powerful antibody profiling capabilities to detect autoantibodies that are associated with systemic autoimmune rheumatic diseases such as systemic lupus erythematosus (SLE), Systemic Sclerosis (SSc), Sjögren’s syndrome (SS), and mixed connective tissue disease (MCTD).

This antigen array contains 32 ENA and ANA antigens which are most relevant to autoimmune disorder. The superior-quality purified nuclear antigens were coated onto the 3D surface of microchips to ensure that antigens retain their natural conformations. Each antigen has 3 different concentrations in duplicates enabling optimal antigen-antibody binding with highest sensitivity and reproducibility for antibody detection. Internal Ig and anti-Ig controls are included in each array for normalization and quantification purpose. Additionally, the utilization of fluorescent 2nd antibodies in our microarrays allow for the detection of different types (and sub-types) of antibodies per experiment, minimizing the requirement of sample needed.

In addition to premade arrays, arrays containing customized sets of antigens are available, as well as array processing kits, array profiling services and data analysis. To order custom arrays and services, please contact us.

GeneCopoeia’s OmicsArray™ ENA and ANA 32-Plex Antigen Array is part of the GeneCopoeia OmicsArray™ Antigen Microarray family.

Reference
  1.  Alsubki, Roua et al. “Association between antinuclear antibodies (ANA) patterns and extractable nuclear antigens (ENA) in HEp-2 cells in patients with autoimmune diseases in Riyadh, Saudi Arabia.” Intractable & rare diseases research vol. 9,2 (2020): 89-94. doi:10.5582/irdr.2020.03012
  2. Tešija Kuna, Andrea et al. “Assessment of antinuclear antibodies (ANA): National recommendations on behalf of the Croatian society of medical biochemistry and laboratory medicine.” Biochemia medica vol. 31,2 (2021): 020502. doi:10.11613/BM.2021.020502
  3. Banhuk, Fernanda Weyand et al. “Relationships among Antibodies against Extractable Nuclear Antigens, Antinuclear Antibodies, and Autoimmune Diseases in a Brazilian Public Hospital.” Autoimmune diseases vol. 2018 9856910. 30 Sep. 2018, doi:10.1155/2018/9856910
  4. Li, Quan-Zhen et al. “Risk factors for ANA positivity in healthy persons.” Arthritis research & therapy vol. 13,2 R38. 2 Mar. 2011, doi:10.1186/ar3271

Premade Antigen Arrays

The listed chip prices apply to academic customers. For custom arrays and services pricing, please contact us

Buy Cat. No.Product NameNumber of AntigensApplicationAntigen ListChip Price ($)Service price
PA008OmicsArray™ Extractable Nuclear Antigen (ENA) and Antinuclear Antibody (ANA) Antigen Array32Profiling autoantibodies associated with systemic autoimmunerheumatic diseasesDownload390Inquiry
User Manual
Prices are for customers from the U.S. and Canada only. For other customers, please contact your local distributor for details.

Antigen microarray processing kits and accessories

Buy Cat. No.Product NameApplicationPrice ($)
PA100OmicsArray™ Antigen Microarray Processing Kit, humanfor processing up to 3 slides, or 45 human samples180
PA101OmicsArray™ Antigen Microarray Processing Kit, mousefor processing up to 3 slides, or 45 mouse samples180
PA105OmicsArray™ Antigen Microarray Slides incubation chamber set3 chambers and 1 rack, for processing 3-slides125
PA106OmicsArray™ Antigen Microarray Slides incubation chamber1 chamber, for processing 1-slide40
User Manual SDS
Prices are for customers from the U.S. and Canada only. For other customers, please contact your local distributor for details.

 

Custom services

GeneCopoeia offers custom antigen microarray services in the following areas:
  • Custom array printing. GeneCopoeia will create custom antigen microarrays built to your specifications.
  • Sample processing. Send us your blood, plasma, tissue, or other biological sample and we will prepare it for processing and incubation with any of our premade antigen microarrays or custom-built antigen microarrays for autoantibody profiling and other applications. For information on sample types to submit, consult the FAQ
  • Data analysis. Once samples are processed and incubated with an antigen microarray, we will analyze the raw data. The standard analysis service includes: 1) An Excel file of the Net Signal Intensity (NSI) for each antigen on the array, normalized to internal controls; and 2) a heat map
Additional analysis services, including proteomic analysis, pathway analysis, and more, are also available.

To inquire about custom antigen microarray products and services, please fill out our custom quote request form.

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