Product Introduction
GeneCopoeia’s BlazeTaq™ Probe One-Step RT-qPCR kit is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as starting material. It offers a convenient master mix to convert RNA to DNA and quantify expression levels in a one step reaction using hydrolysis probes. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components. Tests show that the BlazeTaq™ Probe One-Step RT-qPCR kit is compatible with diverse real-time PCR instruments and outperforms competitors’ products for sensitivity and specificity of RNA detection.
Product details
GeneCopoeia’s BlazeTaq™ Probe One-Step RT-qPCR kit allows potent target RNA detection and quantification using hydrolysis probes in a single tube. RNA is converted to cDNA by a reverse transcriptase in the kit, and cDNA is subsequently amplified by a hot-start Taq DNA polymerase. During DNA amplification, the target-specific probes bound to the target site on DNA are cleaved by the 5′-3′ exonuclease of Taq DNA polymerase. Upon cleavage, the unquenched probes generate fluorescence for real-time quantification.
RT-qPCR targeting ACTB(A), GAPDH(B), and B2M(C) using the BlazeTaq™ Probe One-Step RT-qPCR kit (red) and competitor’s Taqman RT-qPCR kit (blue) with RNA extract from HeLa cells ranging from 10 ng to 0.1 pg in duplicates.
Performance of singleplex and triplex RT-qPCR targeting ACTB(A), GAPDH(B), and B2M(C) using the BlazeTaq™ Probe One-Step RT-qPCR kit with RNA extract from HeLa cells ranging from 100 ng to 0.1 pg in duplicate. The standard curves of singleplex and triplex RT-qPCR show the same amplification efficiency.
Performance comparison of BlazeTaq™ Probe One-Step RT-qPCR kit with competitor’s RT-qPCR kit by the amplification of GAPDH from HeLa cell total RNA extract.
