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BlazeTaq™ SYBR Green qPCR Mix 2.0

Get $50 Amazon gift card* w/ purchase of BlazeTaq qPCR Mix + SureScript Synthesis Kit

BlazeTaq™ SYBR® Green qPCR Mix 2.0 is designed for highly sensitive and accurate quantification of gene expression and real-time PCR reactions. It contains a hot-start antibody-modified Taq DNA polymerase and optimized buffer system that avoids non-specific amplification of target DNA at lower temperatures, and enhances reaction mix performance. The reaction kit offers ready-to-use 5X Master Mix- just add the DNA template, primers and deionized water to start your fast and specific quantitative analysis.

The BlazeTaq™ SYBR® Green qPCR Mix 2.0 can be used in combination with the SureScript™ First-Strand cDNA Synthesis Kit as part of the two-step real-time RT PCR system.

+ Receive $50 Amazon gift card with purchase of BlazeTaq™ SYBR Green qPCR Mix 2.0 + SureScript™ First-Strand cDNA Synthesis Kit!*

Advantages
  • Highly sensitive. Detects as little as 5 copies of DNA template
  • High specificity with minimal level of primer-dimer and non-specific product formation
  • High amplification efficiency over wide GC-content range
Tip: Advantage of antibody-modified over chemically-modified Taq

 

  • Faster heat activation, 30s vs 15 min
  • Less heat-damage to DNA samples
  • More stable performance

 

BlazeTaq™ SYBR® Green qPCR Mix Kits (with or without ROX)

Buy Catalog# Product Description Price
QP031 BlazeTaq™ SYBR Green qPCR Mix 2.0 (200 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $198
QP032 BlazeTaq™ SYBR Green qPCR Mix 2.0 (600 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $465
QP034 BlazeTaq™ SYBR Green qPCR Mix 2.0 (1000 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $760
QP041 BlazeTaq™ SYBR Green qPCR Mix 2.0 (200 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $198
QP042 BlazeTaq™ SYBR Green qPCR Mix 2.0 (600 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $465
QP044 BlazeTaq™ SYBR Green qPCR Mix 2.0 (1000 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $760

 

 

SureScript™ First-Strand cDNA Synthesis Kits

Buy Catalog# Product Description Price
QP056 SureScript™ First-Strand cDNA Synthesis Kit for gene qPCR array (20 RT reactions) M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, dd H2O and spike-in control (for use with gene qPCR arrays) $129
QP057 SureScript™ First-Strand cDNA Synthesis Kit for gene qPCR array (60 synthesis reactions) M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, dd H2O and spike-in control (for use with gene qPCR arrays) $285
  Variable All-in-One™ qPCR Primer
(200 reactions)
Validated gene-specific for human, mouse and rat to ensure specificity and sensitivity
 

 

* Offer is valid in the US & Canada only. Must buy both items to receive gift card. Limit 1 offer per customer per order. Offer ends July 31, 2019

 

BlazeTaq™ SYBR® Green qPCR mix 2.0 uses an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. Upon heating to 95 ºC for 30 seconds, the antibody dissociates and full activity of the Taq polymerase is restored. Also, the optimized buffer system allows high amplification efficiency and specificity, as well as enhanced sensitivity of real time PCR reactions over a wide range of templates.

Features

  • 5X Master Mix for convenient use
  • Hot-start polymerase and optimized buffer system to reduce non-specific reactions
  • Available with or without ROX, allowing compatibility with all commonly used qPCR instruments

Performance

 

Dynamic Range

Wide Dynamic Range with High Sensitivity
DNA templates ranging from 5×107 (blue) to 5 copies (orange) were amplified. Linear regression of fluorescent signal on cycle numbers shows R2 of 99.8% and amplification efficiency of 98.8%.

Reaction Efficiency


Comparison with competitor

Performance Comparison with competitor

Ct value comparison with competitor

Performance comparison of BlazeTaq™ with competitor's kit

 

 

 

 

 

Amplification of the B2M gene using serial diluted cDNA from HeLa cell line. Performance comparison of BlazeTaq™ (red) with BioRad SsoAdvanced™ Universal SYBR® Green Supermix (blue) shows stronger signals with similar amplification performance.

 

Performance comparison of BlazeTaq™ (red) with PowerUp™ SYBR Green Master Mix from Applied Biosystems (green), and GoTaq® qPCR Master Mix from Promega (blue) showed greater sensitivity, amplification efficiency and specificity.

Ct value comparison. Amplification of 41 genes from 10 ng of HeLa cDNA using BlazeTaq™ (blue), PowerUp™ SYBR Green Master Mix from Applied Biosystems (orange), and GoTaq® qPCR Master Mix from Promega (grey).


Low Copy Template Repeated Test

Consistent detection of low copy number DNA templates
Ct values shown for 50 and 5 copies of DNA templates, and no template control (NTC). For each template, the qPCR reactions were repeated 24 times.


GC content vs Efficiency

Amplification efficiencies range from 90% to 110% over a wide range of GC content. DNA templates with different GC-content were serial-diluted and amplified.