SureScript™ First-Strand cDNA Synthesis Kit
SureScript™ First-Strand cDNA Synthesis Kit
Product Introduction
A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from almost any source of RNA
- Efficient and easy
- Reliable
- Cost efficient
The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.
The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
Product details
SureScript™ First-Strand Synthesis Kit in action
The SureScript™ First Strand Synthesis Kit can be used in combination with the BlazeTaq™ SYBR Green qPCR Master Mix as the first step of the two-step RT-qPCR system to convert mRNA into cDNA; the cDNA can be subsequently aliquoted, stored or used for qPCR analysis.
Figure 1. Amplification of actin-β using the BlazeTaq™ two-step RT-qPCR system. A 10-fold dilution series of RNA extracts from HeLa cells were used for cDNA conversion with the SureScript™ First-Strand cDNA Synthesis Kit. The actin-β cDNA was amplified with BlazeTaq™ SYBR® Green qPCR mix 2.0 with amounts ranging from ~100 ng to ~1 pg. </p >
Figure 2. Comparison of the real-time amplication plots of the BlazeTaq Two-Step RT-qPCR System (red) with competitor A’s (A) and competitor B’s (B) products (blue). A 10-fold dilution series of B2M gene from total RNA of Hela cell extract with amounts ranging from 100 ng to 1 pg.
Resources
Resources
Manuals
SureScript™ First-Strand cDNA Synthesis Kit- User Manual
Publications
View selected publications citing GeneCopoeia’s SureScript™ First-Strand cDNA Synthesis Kit from the recent literature
2023
- Zeng, L-P., et al. (2023). Identify GADD45G as a potential target of 4-methoxydalbergione in treatment of liver cancer: bioinformatics analysis and in vivo experiment. World J Surg Oncol. doi: 10.1186/s12957-023-03214-3 [SureScript™ First-Strand cDNA Synthesis Kit]
- Jiang, W., et al. (2023). CHI3L1 signaling impairs hippocampal neurogenesis and cognitive function in autoimmune-mediated neuroinflammation. Sci Adv. doi: 10.1126/sciadv.adg8148 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix]
- Li, S-S., et al. (2023). A new peptide; VD11; promotes structural and functional recovery after spinal cord injury. Neural Regen Res. doi: 10.4103/1673-5374.369119 [SureScript™ First-Strand cDNA Synthesis Kit]
- Ping, Q., et al. (2023). TGF-β1 dominates stromal fibroblast-mediated EMT via the FAP/VCAN axis in bladder cancer cells. J Transl Med doi: 10.1186/s12967-023-04303-3 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR Green qPCR Mix 2.0]
- Zhang, L-M., et al. (2023). Identification of key potassium channel genes of temporal lobe epilepsy by bioinformatics analyses and experimental verification. Front Neurol doi: 10.3389/fneur.2023.1175007 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR Green qPCR Mix 2.0]
- Xie, Y., et al. (2023). Leonurine Alleviates Cognitive Dysfunction and Reduces Oxidative Stress by Activating Nrf-2 Pathway in Alzheimer’s Disease Mouse Model. Neuropsychiatr Dis Treat doi: 10.2147/NDT.S404798 [Surescript™ First-Strand cDNA Synthesis Kit]
- Qin, S., et al. (2023). Positive effects of selenized-oligochitosan on zearalenone-induced intestinal dysfunction in piglets. Front Vet Sci doi: 10.3389/fvets.2023.1184969 [Surescript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Yu, K., et al. (2023). APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN. Front Pharmacol doi: 10.3389/fphar.2023.1181435 [SureScript™ First-Strand cDNA Synthesis Kit]
- Mao, Y., et al. (2023). Identification Invasion-Related Long Non-Coding RNAs in Lung Adenocarcinoma and Analysis of Competitive Endogenous RNA Regulatory Networks. Int J Gen Med doi: 10.2147/IJGM.S407266 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Li, J., et al. (2023). Identification and analysis of hub genes of hypoxia-immunity in type 2 diabetes mellitus. Front Genet doi: 10.3389/fgene.2023.1154839 [Surescript™ First-Strand cDNA Synthesis Kit]
- Liu, W., et al. (2023). Identification and characterization of Dof genes in Cerasus humilis. Front Plant Sci doi: 10.3389/fpls.2023.1152685 [Surescript™ First-Strand cDNA Synthesis Kit]
- Chen, Z., et al. (2023). Polypyrimidine tract binding protein 1 exacerbates cardiac fibrosis by regulating fatty acid‐binding protein 5. ESC Heart Fail doi: 10.1002/ehf2.14318 [RNAzol® RT RNA Isolation Reagent; SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR Green qPCR Mix 2.0]
- Lin, Z-J., et al. (2023). Silencing MYOT Expression May Inhibit Autophagy in Human Skeletal Muscle Cells. Dis Markers doi: 10.1155/2023/3350685 [Surescript™ First-Strand cDNA Synthesis Kit]
- Zhou, J., et al. (2023). LncRNA RPPH1 acts as a molecular sponge for miR-122 to regulate Wnt1/β-catenin signaling in hepatocellular carcinoma. Int J Med Sci doi: 10.7150/ijms.68778 [Surescript™ First-Strand cDNA Synthesis Kit]
- Qin, C., et al. (2023). Transcriptome-wide analysis reveals the molecular mechanisms of cannabinoid type II receptor agonists in cardiac injury induced by chronic psychological stress. Front Genet doi: 10.3389/fgene.2022.1095428 [Surescript™ First-Strand cDNA Synthesis Kit]
- Qu, A., et al. (2023). Prognostic and predictive value of a lncRNA signature in patients with stage II colon cancer. Sci Rep doi: 10.1038/s41598-022-25852-5 [Surescript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Kong, G., et al. (2023). Adenovirus-IL-10 relieves chronic rejection after mouse heart transplantation by inhibiting miR-155 and activating SOCS5. Int J Med Sci doi: 10.7150/ijms.77093 [SureScript™ First-Strand cDNA Synthesis Kit]
- Ma, J., et al. (2023). Irisin Ameliorates PM2.5-Induced Acute Lung Injury by Regulation of Autophagy Through AMPK/mTOR Pathway. J Inflamm Res doi: 10.2147/JIR.S390497 [Surescript™ First-Strand cDNA Synthesis Kit]
- Gong, Q., et al. (2023). Construction and validation of an angiogenesis-related lncRNA prognostic model in lung adenocarcinoma. Front Genet doi: 10.3389/fgene.2023.1083593 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Zhao, J., et al. (2023). Construction of a prognostic model of luteolin for endometrial carcinoma. Am J Transl Res [SureScript™ First-Strand cDNA Synthesis Kit]
- Chen, K., et al. (2023). Identification of feature genes and key biological pathways in immune-mediated necrotizing myopathy: High-throughput sequencing and bioinformatics analysis. Comput Struct Biotechnol J doi: 10.1016/j.csbj.2023.03.019 [SureScript™ First-Strand cDNA Synthesis Kit]
- Yuan, M., et al. (2023). Long noncoding RNA Mhrt alleviates angiotensin II-induced cardiac hypertrophy phenotypes by mediating the miR-765/Wnt family member 7B pathway. Open Med (Wars) doi: 10.1515/med-2023-0681 [All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0; SureScript™ First-Strand cDNA Synthesis Kit; All-in-One™ miRNA qRT-PCR Detection Kit 2.0; All-in-One™ qPCR Mix]
2022
- Qin, S., et al. (2022). The comprehensive expression and functional analysis of m6A modification “readers” in hepatocellular carcinoma. Aging (Albany NY) doi: 10.18632/aging.204217 [SureScript™ First-Strand cDNA Synthesis Kit]
- Wang, H-F., et al. (2022). Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma. Cancers (Basel) doi: 10.3390/cancers14174151 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR Green qPCR mix 2.0]
- Yin, S., et al. (2022). Peptide OM-LV20 protects astrocytes against oxidative stress via the ‘PAC1R/JNK/TPH1’ axis. J Biol Chem doi: 10.1016/j.jbc.2022.102429 [SureScript™ First-Strand cDNA Synthesis Kit]
- Pan, Y-Q., et al. (2022). Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma. Front Oncol doi: 10.3389/fonc.2022.1071100 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR Mix 2.0]
- Deng, H.X., et al. (2022). Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway. Anal Cell Pathol (Amst) doi: 10.1155/2022/4588999 [SureScript™ First-Strand cDNA Synthesis Kit]
- Dongm, X.M., et al. (2022). Identification of optimal reference genes for gene expression normalization in human osteosarcoma cell lines under proliferative conditions. Front Genet doi: 10.3389/fgene.2022.989990 [SureScript™ First-Strand cDNA Synthesis Kit]
- Lin, C., et al. (2022). Integrating RNA-seq and scRNA-seq to explore the biological significance of NAD + metabolism-related genes in the initial diagnosis and relapse of childhood B-cell acute lymphoblastic leukemia. Front Immunol doi: 10.3389/fimmu.2022.1043111 [SureScript™ First-Strand cDNA Synthesis Kit]
- Li, z.h., et al. (2022). Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes. Int J Mol Sci doi: 10.3390/ijms232314607 [SureScript™ First-Strand cDNA Synthesis Kit]
- Jiang, H., et al. (2022). Bioinformatics identification and validation of biomarkers and infiltrating immune cells in endometriosis. Front Immunol doi: 10.3389/fimmu.2022.944683 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Zhao, J.D., et al. (2022). Construction of N-7 methylguanine-related mRNA prognostic model in uterine corpus endometrial carcinoma based on multi-omics data and immune-related analysis. Sci Rep doi: 10.1038/s41598-022-22879-6 [SureScript™ First-Strand cDNA Synthesis Kit]
- Zhang, Q.Y., et al. (2022). Screening and validation of lymph node metastasis risk-factor genes in papillary thyroid carcinoma. Front Endocrinol (Lausanne) doi: 10.3389/fendo.2022.991906 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Lei, L., et al. (2022). Comprehensive Diagnostics of Diabetic Nephropathy by Transcriptome RNA Sequencing. Diabetes Metab Syndr Obes doi: 10.2147/DMSO.S371026 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Pan, Y-Q., et al. (2022). Prognostic value of lncRNAs related to fatty acid metabolism in lung adenocarcinoma and their correlation with tumor microenvironment based on bioinformatics analysis. Front Oncol doi: 10.3389/fonc.2022.1022097 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ qPCR Mix]
- Liu, Q.S., et al. (2022). Construction and Validation of Pyroptosis-Related lncRNA Prediction Model for Colon Adenocarcinoma and Immune Infiltration Analysis. Dis Markers doi: 10.1155/2022/4492608 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Liu, C., et al. (2022). Prognostic Modeling of Lung Adenocarcinoma Based on Hypoxia and Ferroptosis-Related Genes. J Oncol doi: 10.1155/2022/1022580 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ One-Step SYBR Green RT-qPCR Kit]
- Sun, L.H., et al. (2022). The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission. Oxid Med Cell Longev doi: 10.1155/2022/3639302 [SureScript™ First-Strand cDNA Synthesis Kit]
- Tan, L.J., et al. (2022). Peripheral Transplantation of Mesenchymal Stem Cells at Sepsis Convalescence Improves Cognitive Function of Sepsis Surviving Mice. Oxid Med Cell Longev doi: 10.1155/2022/6897765 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Bai, R.J., et al. (2022). Exploring the biological function of immune cell-related genes in human immunodeficiency virus (HIV)-1 infection based on weighted gene co-expression network analysis (WGCNA). BMC Med Genomics doi: 10.1186/s12920-022-01357-y [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
- Luo, S-M., et al. (2022). Dexmedetomidine exerts an anti-inflammatory effect via α2 adrenoceptors to alleviate cognitive dysfunction in 5xFAD mice. Front Aging Neurosci doi: 10.3389/fnagi.2022.978768 [SureScript™ First-Strand cDNA Synthesis Kit]
- Bai, F., et al. (2022). Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy. Front Genet doi: 10.3389/fgene.2022.965816 [SureScript™ First-Strand cDNA Synthesis Kit]
- Xu, X., et al. (2022). ECM-inspired 3D printed polyetherimide scaffold with Arg-Gly-Asp peptides for the improvement of bioactivity and osteogenic differentiation of osteoblasts. Materials Today Communications doi: 10.1016/j.mtcomm.2022.103166 [SureScript™ First Strand cDNA Synthesis Kit]
- Huang, Z., et al. (2022). Mechanism of ketotifen fumarate inhibiting renal calcium oxalate stone formation in SD rats. Biomedicine & Pharmacotherapy doi: 10.1016/j.biopha.2022.113147 [SureScript™ First Strand cDNA Synthesis Kit]
- Ma, M., et al. (2022). Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments. Cancer Medicine doi: 10.1002/cam4.4897 [SureScript™ First Strand cDNA Synthesis Kit]
- Zha, W., et al. (2022). Genome-wide identification of long non-coding (lncRNA) in Nilaparvata lugens’s adaptability to resistant rice. Zoological Science doi: 10.7717/peerj.13587 [SureScript™ First Strand cDNA Synthesis Kit]
- Bai, Z., et al. (2022). ERCC5, HES6 and RORA are potential diagnostic markers of coronary artery disease. FEBS open bio doi: 10.1002/2211-5463.13469 [SureScript™ First Strand cDNA Synthesis Kit]
2021
- Qi, J-l., et al. (2021). SQSTM1/p62 regulate breast cancer progression and metastasis by inducing cellcycle arrest and regulating immune cell infiltration. Genes & Diseases doi: 10.1016/j.gendis.2021.03.008 [SureScript™ First Strand cDNA Synthesis Kit]
- Li, J., et al. (2021). Biliverdin Protects Against Cerebral Ischemia/ Reperfusion Injury by Regulating the miR-27a-3p/ Rgs1 Axis. Neuropsychiatric Disease and Treatment 2021:17 1165–1181 [SureScript™ First Strand cDNA Synthesis Kit]
- Wang, D., et al. (2021). TGM2 positively regulates myoblast differentiation via enhancing the mTOR signaling. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research doi: 10.1016/j.bbamcr.2021.119173 [SureScript™ First Strand cDNA Synthesis Kit]
- Liu, X., et al. (2021). Dihydroquercetin suppresses cigarette smoke induced ferroptosis in the pathogenesis of chronic obstructive pulmonary disease by activating Nrf2-mediated pathway. Phytomedicine doi: 10.1016/j.phymed.2021.153894 [All-in-One™ qPCR Mix]
2020
- Shi, M., et al. (2020). lncRNA-Associated ceRNA Networks in Spleen of Nocth1-correlated T-ALL Leukemia Mice. BMC Cancer doi: 10.21203/rs.2.21227/v2 [SureScript™ First Strand cDNA Synthesis Kit]
- Cao, X., et al. (2020).The DNMT1/miR-34a/FOXM1 Axis Contributes to Stemness of Liver Cancer Cells. Journal of Oncology doi: 10.1155/2020/8978930 [SureScript™ First Strand cDNA Synthesis Kit]
- Komnenov, D., et al. (2020). V1a and V1b Vasopressin Receptors Within the Paraventricular Nucleus Contribute to Hypertension in Male Rats Exposed to Chronic Mild Unpredictable Stress. American Journal of Physiology – Regulatory, Integrative and Comparative Phsiology doi: 10.1152/ajpregu.00245.2020 [SureScript™ First Strand cDNA Synthesis Kit]
2019
- Jin, X., et al. (2019). MicroRNA-486-5p improves nonsmall-cell lung cancer chemotherapy sensitivity and inhibits epithelial–mesenchymal transition by targeting twinfilin actin binding protein 1 . Journal of International Medical Research doi: 10.1177/0300060519850739 [SureScript™ First Strand cDNA Synthesis Kit]
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