You are here: Home > Products >

miProfile™ Custom miRNA qPCR Arrays

For expression profiling of custom-specified miRNAs

miProfile™ Custom miRNA qPCR Arrays profile the expression of customer specified miRNAs. Each primer is designed using a proprietary algorithm and has been experimentally validated. Depending on the number of miRNAs and replicates to be analyzed, GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility:

  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.
  • Negative controls(NC): monitors the sample quality

For the best performance, All-in-One™ miRNA First-Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

To view other miProfile miRNA qPCR arrays, click below:

 

Key advantages

Request a Quote

Validated primers

  • Each primer is designed using a proprietary algorithm and has been experimentally  validated

Flexible array design

  • GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate.

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer has been experimental validated for specific amplification
  • Broad linearity – Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicates

 

Cat. No. Product name Species miRNAs Number of plates Document
PAM-CS96 miProfile™ miRNA qPCR arrays custom services N/A Variable Variable Variable

Select RT-PCR reagents

Buy Catalog# Product Description Price
QP001 All-in-One™ qPCR Mix (200 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs inquire
QP002 All-in-One™ qPCR Mix (600 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs inquire
QP004 All-in-One™ qPCR Mix (1000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs inquire
QP005 All-in-One™ qPCR Mix (4000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs inquire
QP017 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array (20 RT reactions) Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) inquire
QP018 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array (60 RT reactions) Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) inquire
QP020 RNAzol® RT RNA Isolation Reagent Easy isolation of mRNA, microRNA or total RNA inquire
QP115 All-in-One™ miRNA qRT-PCR Detection Kit 2.0* (20 RT and 200 qPCR reactions) Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) inquire
QP116 All-in-One™ miRNA qRT-PCR Detection Kit 2.0* (60 RT and 600 qPCR reactions) Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) inquire

 

 

 

 

 

 

 

 

 

 

 

 

Data analysis tool

Analyze the qPCR result using GeneCopoeia's online Data Analysis System (free).

Related products