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miProfile™ Custom miRNA qPCR Arrays

For expression profiling of custom-specified miRNAs

miProfile™ Custom miRNA qPCR Arrays profile the expression of customer specified miRNAs. Each primer is designed using a proprietary algorithm and has been experimentally validated. Depending on the number of miRNAs and replicates to be analyzed, GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility:

  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.
  • Negative controls(NC): monitors the sample quality

For the best performance, All-in-One™ miRNA First-Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

To view other miProfile miRNA qPCR arrays, click below:


Key advantages

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Validated primers

  • Each primer is designed using a proprietary algorithm and has been experimentally  validated

Flexible array design

  • GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate.

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer has been experimental validated for specific amplification
  • Broad linearity – Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicates

 

Cat. No. Product name Species miRNAs Number of plates Document
PAM-CS96 miProfile™ miRNA qPCR arrays custom services N/A Variable Variable Variable

Select RT-PCR reagents

Buy Catalog# Product Description Price
EP001 ExoSure™ Solution Optimized precipitation solution for exosome isolated. $185
EP002 ExoSure™ Exosome Isolation Kit Optimized precipitation solution and SEC technique based columns for exosome isolated. $459
QP029 All-in-One™ miRNA Universal Adaptor PCR Primer miRNA Universal Adaptor PCR Primer (use with All-in-One™ miRNA First-Strand cDNA Synthesis kit 2.0 QP117 or QP118) $59
QP115 All-in-One™ miRNA qRT-PCR Detection Kit 2.0 Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) $395
QP116 All-in-One™ miRNA qRT-PCR Detection Kit 2.0 Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) $985
QP117 All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $225
QP118 All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $515
QP125 ExoCt™ First-Strand cDNA Synthesis Kit ExoCt™ Lysis Buffer, ExoCt™ RT-for-All™ Buffer, ExoCt™ PAP/RTase Mix $280
QP126 ExoCt™ First-Strand cDNA Synthesis Kit ExoCt™ Lysis Buffer, ExoCt™ RT-for-All™ Buffer, ExoCt™PAP/RTase Mix $800
QP131 ExoCt™ SYBR® Green RT-qPCR Kit ExoCt™ Lysis Buffer, ExoCt™ RT-for-All™ Buffer, ExoCt™ PAP/RTase Mix, BlazeTaq™ qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer $458
QP132 ExoCt™ SYBR® Green RT-qPCR Kit ExoCt™ Lysis Buffer, ExoCt™ RT-for-All™ Buffer, ExoCt™ PAP/RTase Mix, BlazeTaq™ qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer $1230
User Manual

 

Data analysis tool

Analyze the qPCR result using GeneCopoeia's online Data Analysis System (free).

 

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