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BlazeTaq™ One-Step SYBR Green RT-qPCR Kit

BlazeTaq™ One-Step SYBR® Green RT-qPCR kit is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one step reaction. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components. Tests show that the BlazeTaq™ One-Step SYBR® Green RT-qPCR kit is compatible with diverse real-time PCR instruments and outperforms competitors' products on the sensitivity and specificity of RNA detection.

Advantages
  • High sensitivity- accurate detection of target RNA from as little as 0.1 pg RNA extract
  • High specificity, with minimal level of primer-dimer and non-specific product formation
  • High amplification efficiency over wide GC-content range

BlazeTaq SYBR Green RT-qPCR Kit

 

BlazeTaq™ One-Step SYBR® Green RT-qPCR Kits (with or without ROX)

 

Buy Catalog# Product Description Price
QP070 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (100 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $119
QP071 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (200 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $239
QP072 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (600 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $559
QP074 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (1000 rxn, with ROX) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $910
QP080 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (100 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $119
QP081 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (200 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $239
QP082 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (600 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $559
QP084 BlazeTaq™ One-Step SYBR Green RT-qPCR Kit (1000 rxn, w/o ROX) High-fidelity, hot-start DNA polymerase, optimized reaction buffer and dNTPs $910
  Variable All-in-One™ qPCR Primer
(200 reactions)
Validated gene-specific for human, mouse and rat to ensure specificity and sensitivity
 

 

 

 

BlazeTaq™ One-Step SYBR® Green RT-qPCR kit uses a reverse transcriptase to convert RNA to cDNA, and an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. Upon heating to 95 ºC for 3 min, the antibody dissociates and full activity of the Taq polymerase is restored. Also, the optimized buffer system allows high amplification efficiency and specificity, as well as enhanced sensitivity of real time PCR reactions over a wide range of templates. Check out the performance comparison of BlazeTaq™ One-Step RT-qPCR kit with competitors' kits.

Features

  • One-step reaction to quantify the relative amount of RNA
  • Hot-start polymerase and optimized buffer system to reduce non-specific reactions
  • Available with or without ROX, allowing compatibility with all commonly used qPCR instruments

Performance

Figure 1. Analysis of the level of GAPDH and Actin in RNA extract from HeLa cells. A. Amplification curves of GAPDH and Actin respectively in HeLa cells RNA extract ranging from 100 ng to 0.1 pg; B. Amplification curves of GAPDH and Actin respectively in HeLa cells RNA extract ranging from 100 ng to 0.1 pg;C. Melting curves of the amplified fragments of GAPDH (red) and Actin (blue).

BlazeTaq RT-qPCR Amplification Plots
BlazeTaq RT-qPCR Melting Curves

 

 

 

 

 

 

 

Figure 2. Comparison of the performance of BlazeTaq™ RT-qPCR kit with competitor's product. A. Amplification curves of GAPDH in HeLa cells RNA extract ranging from 100 ng to 0.1 pg with BlazeTaq™ (red) and Competitor T's kit (blue); B. Amplification curves of Actin in HeLa cells RNA extract ranging from 100 ng to 0.1 pg with BlazeTaq™ (red) and Competitor T's kit (blue).

BlazeTaq RT-qPCR Sensitivity Comparison with Competitor Kit

 

Figure 3. Comparison of the performance of BlazeTaq™ RT-qPCR kit with competitor's product. A. Amplification curves of GAPDH in HeLa cells RNA extract ranging from 100 ng to 0.1 pg with BlazeTaq™ (red) and Competitor P's kit (blue); B. Melting curves of amplified fragments of GAPDH with BlazeTaq™ (red) and Competitor P kit (blue). The peak shows non-specific amplification from competitor P's kit.

BlazeTaq RT-qPCR Specificity Comparison with Competitor Kit