GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for expressing or modifying genes in a broad range of cell and tissue types, especially in vivo, with high efficiency and enhanced safety. GeneCopoeia’s optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made AAV are available in 25 different serotypes, such as fluorescent reporters. You can also request us to produce custom AAV for genes up to 3 kb in length.
High titers. Titer of purified AAV particles can be up to 1014 GC/ml.
High adaptability. Packaging types include-ORF cDNA, CRISPR, shRNA, or miRNA.
Low toxicity. Does not integrate into the host genome.
Low immunogenicity. Minimal host immune response.
Safe. Not associated with any human disease
Search all AAV products by gene ID or accession number
*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
AAVPrime™ uses a helper virus-free system for the safe preparation of high titer AAV. HEK293T cells are co-transfected with three plasmids encoding the factors necessary for recombinant AAV packaging. The AAV particles are then either membrane purified or purified by two-phase partitioning for high-titer AAV ready for in vivo animal use.
The destination vector carries your gene of interest (GOI) and two ITRs. You can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and other tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection.(Learn more about our vector collection); the plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.
Figure 1. Purified eGFP AAV-9 Adeno-associated Viral Particles (Cat.No. AA016-100) was injected intravenously into adult mice at a dose of 1013 GC/kg (2-3 ×1011 GC/ mouse), and tissue sections and fluorescence photography were performed after 2 weeks.
Figure 2. AAV of different serotypes carrying GFP were used to transduce the H1299 cell line as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.
Figure 3. MyoAAV and AAV-MG indicated its tissue specificity in vitro infection. AAV-9, MyoAAV and AAV-MG carrying GFP were used to transduce the Rhabdomyosarcoma (RD) cell line(left) or Human microglia cell line (HmC3)(right).
AAV Serotype Choices
GeneCopoeia provides AAV for all the commonly-occurring AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, AAV13, AAV-DJ, AAV-DJ/8, MyoAAV, AAV-MG, AAV-PHP.eB and AAV-BI30, AAV-PHP.S, AAV2.7m8, AAV2-QuadYF, AAV2-retro, AAV6.2 and AAV9-insALAVPFR. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different tissues.
Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to compare the essential differences between different serotypes.
AAV Serotypes Available
Serotype
Primary Target Tissues
Description
AAV-1
Muscle
Best for cardiac muscle, skeletal muscle, neuronal and glial tissue.
AAV-2
Muscle, Liver, Retina
Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain.
AAV-3
Megakaryocytes
Best for megakaryocytes, muscle, liver, lung, and retina.
AAV-4
Retina
Best for neurons, muscle, brain, and retina.
AAV-5
Lung
Best for lung, neurons, synovial joint, retina.
AAV-6
Muscle, Lung
Best for lung, liver, and heart.
AAV-7
Muscle, Retina, Neurons
Best for muscle, neurons, and liver.
AAV-8
Liver
Best for muscle, brain, liver, retina, pancreas and adipose.
AAV-9
Various
Best for muscle, heart, liver, lung, and brain.
AAV-10
Pleura, CNS
Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver.
AAV-11 NEW!
Spinal nerves
Efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction.
AAV-12 NEW!
Salivary glands, Skeletal muscles
Does not depend on either heparan sulfate or sialic acid for transduction. Transduces salivary glands and skeletal muscles in vivo.
AAV-13 NEW!
CNS
Suitable for precise labeling, especially for targeting small nuclei in the brain.
AAV-DJ
Various
A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro.
AAV-DJ/8
Various
A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo.
MyoAAV NEW!
Muscle tissue
Best specificity for muscle tissue.
AAV-MG NEW!
Microglia
Best specificity for microglia in the brain.
AAV-PHP.eB NEW!
CNS
Cross the blood–brain barrier (BBB) and has enhanced CNS tropism.
AAV-BI30 NEW!
CNS
Specifically and efficiently transduces endothelial cells throughout the CNS.
AAV-PHP.S NEW!
PNS
Effective infection of peripheral nervous system.
AAV2.7m8 NEW!
Retina, inner ear
Effective infection of retinal cells, inner ear cochlear hair cells.
AAV2-QuadYF NEW!
Endothelial cell, retina
Effective infection of retinal cells, vascular endothelial cells.
AAV2-retro NEW!
Spinal nerves
Effective retrograde transduction of selective corticospinal tract neurons.
AAV6.2 NEW!
Retina, lung
Effective infection of retina, lung and the airway epithelium.
AAV9-insALAVPFR NEW!
Macrophage
Effective infection of non-CNS tissue-resident macrophages (TRMs).
We offer AAV of virtually all serotypes with titers starting from 5×1012 GC/ml packaged with ORF cDNA, CRISPR/Cas9 or shRNA. Click the links below to search for your gene(s) of interest.
For custom AAV packaging service, please contact us at inquiry@genecopoeia.comto get a quote.
Medium-strength promoter, human phosphoglycerate kinase 1 promoter.
N/A
511
p90
UBC
Weak promoter, human ubiquitin C promoter.
N/A
1212
s00
CAMKlla
Highly-specific expression in excitatory neurons in the neocortex and hippocampus.
N/A
1291
s10
hSyn
Human synapsin-1 gene promoter, neuronal-specific.
N/A
463
s20
GFAP
Human glial fibrillary acidic protein promoter. Tissue specificity: Brain. Cell type specificity: Astrocytes.
N/A
681
s30
aMHC
Muscle-specific human promoter.
N/A
367
s40
MHCK7
Composed of enhancer/promoter regions of murine muscle creatine kinase (MCK) and enhancer region of α-myosin heavy-chain genes. Tissue specificity: mouse skeletal and heart muscles. Cell type specificity: differentiated postmitotic striated muscle cells.
N/A
771
s50
ALB
Liver-specific human promoter.
N/A
2337
s60
TBG
Human thyroxine-binding globulin promoter. Tissue specificity: Liver. Cell type specificity: Hepatocytes.
N/A
479
s70
cTnT
Chicken cardiac troponin T promoter. Tissue specificity: Heart. Cell type specificity: Cardiomyocytes.
N/A
565
s80
MBP
Oligodendrocyte-specific expression in central nervous system.
N/A
1311
s90
MCK
Cardiac muscle-specific human promoter.
N/A
1359
p11
CMV + N-3xFLAG
CMV immediate early enhancer/promoter, 3 tandem FLAG epitope tag, Facilitates antibody binding of tagged protein.
N-3xFLAG
670
p12
CMV + N-3xHA
CMV immediate early enhancer/promoter, 3 tandem Hemagglutinin epitope tag, Facilitates antibody binding of tagged protein..
N-3xHA
694
p13
CMV + N-Myc
CMV immediate early enhancer/promoter, c-Myc epitope tag, Facilitates antibody binding of tagged protein.
N-Myc
637
p14
CMV + N-Avi
CMV immediate early enhancer/promoter, facilitates biotinylation of tagged protein by the enzyme BirA so the protein can be isolated by streptavidin.
N-Avi
655
p15
CMV + N-eGFP
CMV immediate early enhancer/promoter, commonly used green fluorescent protein; ranked high in brightness, photostability and pH stability among all fluorescent proteins.
N-eGFP
1318
p16
CMV + N-mcherry
CMV immediate early enhancer/promoter, commonly used red fluorescent protein; fast maturation compared to its predecessor, mRFP1.
N-mcherry
1309
p17
CMV + N-Halo
CMV immediate early enhancer/promoter, increases expression and solubility of tagged protein; enables covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin; high yield and purity.
N-Halo
1525
p18
CMV + N-SNAP
CMV immediate early enhancer/promoter, allows to observe localization and translocation of SNAP-tag fusion proteins, CLIP and SNAP fusion proteins can be simultaneously and specifically labeled with different synthetic probes within cells.
N-SNAP
1147
p19
CMV + N-His6
CMV immediate early enhancer/promoter, Facilitates purification by nickel or cobalt matrix and antibody binding of tagged protein.
N-His6
631
p21
CMV with intron + N-3xFLAG
CMV immediate early enhancer/promoter with chimeric intron, 3 tandem FLAG epitope tag, Facilitates antibody binding of tagged protein.
N-3xFLAG
1070
p22
CMV with intron + N-3xHA
CMV immediate early enhancer/promoter with chimeric intron, 3 tandem Hemagglutinin epitope tag, Facilitates antibody binding of tagged protein..
N-3xHA
1094
p23
CMV with intron + N-Myc
CMV immediate early enhancer/promoter with chimeric intron, c-Myc epitope tag, Facilitates antibody binding of tagged protein.
N-Myc
1037
p24
CMV with intron + N-Avi
CMV immediate early enhancer/promoter with chimeric intron, facilitates biotinylation of tagged protein by the enzyme BirA so the protein can be isolated by streptavidin.
N-Avi
1055
p25
CMV with intron + N-eGFP
CMV immediate early enhancer/promoter with chimeric intron, commonly used green fluorescent protein; ranked high in brightness, photostability and pH stability among all fluorescent proteins.
N-eGFP
1718
p26
CMV with intron + N-mcherry
CMV immediate early enhancer/promoter with chimeric intron, commonly used red fluorescent protein; fast maturation compared to its predecessor, mRFP1.
N-mcherry
1709
p27
CMV with intron + N-Halo
CMV immediate early enhancer/promoter with chimeric intron, increases expression and solubility of tagged protein; enables covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin; high yield and purity.
N-Halo
1925
p28
CMV with intron + N-SNAP
CMV immediate early enhancer/promoter with chimeric intron, allows to observe localization and translocation of SNAP-tag fusion proteins, CLIP and SNAP fusion proteins can be simultaneously and specifically labeled with different synthetic probes within cells.
N-SNAP
1547
p29
CMV with intron + N-His6
CMV immediate early enhancer/promoter with chimeric intron, Facilitates purification by nickel or cobalt matrix and antibody binding of tagged protein.
N-His6
1031
p41
EF1a + N-3xFLAG
Human eukaryotic translation elongation factor 1 α1 promoter, 3 tandem FLAG epitope tag, Facilitates antibody binding of tagged protein.
N-3xFLAG
1260
p42
EF1a + N-3xHA
Human eukaryotic translation elongation factor 1 α1 promoter, 3 tandem Hemagglutinin epitope tag, Facilitates antibody binding of tagged protein..
N-3xHA
1284
p43
EF1a + N-Myc
Human eukaryotic translation elongation factor 1 α1 promoter, c-Myc epitope tag, Facilitates antibody binding of tagged protein.
N-Myc
1227
p44
EF1a + N-Avi
Human eukaryotic translation elongation factor 1 α1 promoter, facilitates biotinylation of tagged protein by the enzyme BirA so the protein can be isolated by streptavidin.
N-Avi
1245
p45
EF1a + N-eGFP
Human eukaryotic translation elongation factor 1 α1 promoter, commonly used green fluorescent protein; ranked high in brightness, photostability and pH stability among all fluorescent proteins.
N-eGFP
1908
p46
EF1a + N-mcherry
Human eukaryotic translation elongation factor 1 α1 promoter, commonly used red fluorescent protein; fast maturation compared to its predecessor, mRFP1.
N-mcherry
1899
p47
EF1a + N-Halo
Human eukaryotic translation elongation factor 1 α1 promoter, increases expression and solubility of tagged protein; enables covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin; high yield and purity.
N-Halo
2115
p48
EF1a + N-SNAP
Human eukaryotic translation elongation factor 1 α1 promoter, allows to observe localization and translocation of SNAP-tag fusion proteins, CLIP and SNAP fusion proteins can be simultaneously and specifically labeled with different synthetic probes within cells.
N-SNAP
1737
p49
EF1a + N-His6
Human eukaryotic translation elongation factor 1 α1 promoter, Facilitates purification by nickel or cobalt matrix and antibody binding of tagged protein.
N-His6
1221
p4a
EF1a
Human eukaryotic translation elongation factor 1 α1 promoter, eYFP is an enhanced yellow fluorescent protein.
N-eYFP
1908
p51
CAG + N-3xFLAG
CMV early enhancer fused to modified chicken β-actin promoter, 3 tandem FLAG epitope tag, Facilitates antibody binding of tagged protein.
N-3xFLAG
1805
p52
CAG + N-3xHA
CMV early enhancer fused to modified chicken β-actin promoter, 3 tandem Hemagglutinin epitope tag, Facilitates antibody binding of tagged protein..
N-3xHA
1829
p53
CAG + N-Myc
CMV early enhancer fused to modified chicken β-actin promoter, c-Myc epitope tag, Facilitates antibody binding of tagged protein.
N-Myc
1772
p54
CAG + N-Avi
CMV early enhancer fused to modified chicken β-actin promoter, facilitates biotinylation of tagged protein by the enzyme BirA so the protein can be isolated by streptavidin.
N-Avi
1790
p55
CAG + N-eGFP
CMV early enhancer fused to modified chicken β-actin promoter, commonly used green fluorescent protein; ranked high in brightness, photostability and pH stability among all fluorescent proteins.
N-eGFP
2453
p56
CAG + N-mcherry
CMV early enhancer fused to modified chicken β-actin promoter, commonly used red fluorescent protein; fast maturation compared to its predecessor, mRFP1.
N-mcherry
2444
p57
CAG + N-Halo
CMV early enhancer fused to modified chicken β-actin promoter, increases expression and solubility of tagged protein; enables covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin; high yield and purity.
N-Halo
2660
p58
CAG + N-SNAP
CMV early enhancer fused to modified chicken β-actin promoter, allows to observe localization and translocation of SNAP-tag fusion proteins, CLIP and SNAP fusion proteins can be simultaneously and specifically labeled with different synthetic probes within cells.
N-SNAP
2282
p59
CAG + N-His6
CMV early enhancer fused to modified chicken β-actin promoter, Facilitates purification by nickel or cobalt matrix and antibody binding of tagged protein.
Choose from our collection of AAV vectors with different promoters and fluorescent markers for your gene expression. The AAV can be packaged with ORF cDNA clones up to 3 kb in length for long-term expression.
If none of our ORF expression-ready AAV vectors is suitable for your research, please use GeneCopoeia’s V-Architect tools which provides various promoters and tags to match your experimental design.
Transduction-ready AAV delivering shRNA of varying lengths (19 to 29 bases) that were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect.
By default, all options for AAV serotype, gene of interest, and volume are listed in the table below. Use the drop-down menus to narrow down the number of options displayed.