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AAVPrime™ Adeno-associated Virus (AAV)

GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for expressing or modifying genes in a broad range of cell and tissue types, especially in vivo, with high efficiency and enhanced safety. GeneCopoeia’s optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made AAV are available in 23 different serotypes, such as fluorescent reporters. You can also request us to produce custom AAV for genes up to 3 kb in length.

  • High titers. Titer of purified AAV particles can be up to 1014 GC/ml.
  • High adaptability. Packaging types include-ORF cDNA, CRISPR, shRNA, or miRNA.
  • Versatile. Serotypes includes AAV-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, DJ, DJ/8, MyoAAV, AAV-MG, PHP.eB, BI30, PHP.S, AAV2.7m8, AAV2-QuadYF and AAV2-retro.
  • Low toxicity. Does not integrate into the host genome.
  • Low immunogenicity. Minimal host immune response.
  • Safe. Not associated with any human disease
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Technology overview

AAV Technology Overview

Custom AAV Production with the AAVPrime™ System

AAVPrime™ uses a helper virus-free system for the safe preparation of high titer AAV. HEK293T cells are co-transfected with three plasmids encoding the factors necessary for recombinant AAV packaging. The AAV particles are then either membrane purified or purified by two-phase partitioning for high-titer AAV ready for in vivo animal use.
AAV packaging plasmids The destination vector carries your gene of interest (GOI) and two ITRs. You can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and other tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection. (Learn more about our vector collection); the plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.
Figure 1. AAV of different serotypes carrying GFP were used to transduce the H1299 cell line as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.
Figure 2. MyoAAV and AAV-MG indicated its tissue specificity in vitro infection. AAV-9, MyoAAV and AAV-MG carrying GFP were used to transduce the Rhabdomyosarcoma (RD) cell line(left) or Human microglia cell line (HmC3)(right).
AAV Serotype Choices
GeneCopoeia provides AAV for all the commonly-occurring AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, AAV13, AAV-DJ, AAV-DJ/8, MyoAAV, AAV-MG, AAV-PHP.eB and AAV-BI30, AAV-PHP.S, AAV2.7m8, AAV2-QuadYF, AAV2-retro. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different tissues.
Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to compare the essential differences between different serotypes.

AAV Serotypes Available

Serotype Primary Target Tissues Description
AAV-1 Muscle Best for cardiac muscle, skeletal muscle, neuronal and glial tissue.
AAV-2 Muscle, Liver, Retina Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain.
AAV-3 Megakaryocytes Best for megakaryocytes, muscle, liver, lung, and retina.
AAV-4 Retina Best for neurons, muscle, brain, and retina.
AAV-5 Lung Best for lung, neurons, synovial joint, retina, and pancreas.
AAV-6 Muscle, Lung Best for lung, liver, and heart.
AAV-7 Muscle, Retina, Neurons Best for muscle, neurons, and liver.
AAV-8 Liver Best for muscle, brain, liver, and retina.
AAV-9 Various Best for muscle, heart, liver, lung, and brain.
AAV-10 Pleura, CNS Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver.
AAV-11 NEW! Spinal nerves Efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction.
AAV-12 NEW! Salivary glands, Skeletal muscles Does not depend on either heparan sulfate or sialic acid for transduction. Transduces salivary glands and skeletal muscles in vivo.
AAV-13 NEW! CNS Suitable for precise labeling, especially for targeting small nuclei in the brain.
AAV-DJ Various A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro.
AAV-DJ/8 Various A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo.
MyoAAV NEW! Muscle tissue Best specificity for muscle tissue.
AAV-MG NEW! Microglia Best specificity for microglia in the brain.
AAV-PHP.eB NEW! CNS Cross the blood–brain barrier (BBB) and has enhanced CNS tropism.
AAV-BI30 NEW! CNS Specifically and efficiently transduces endothelial cells throughout the CNS.
AAV-PHP.S NEW! PNS Effective infection of peripheral nervous system.
AAV2.7m8 NEW! Retina, inner ear Effective infection of retinal cells, inner ear cochlear hair cells.
AAV2-QuadYF NEW! Endothelial cell, retina Effective infection of retinal cells, vascular endothelial cells.
AAV2-retro NEW! Spinal nerves Effective retrograde transduction of selective corticospinal tract neurons.