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    Lentifect™ Lentivirus

    GeneCopoeia’s Lentifect™ purified and ultra-purified lentivirus for in vitro and in vivo use are available either custom-made or pre-made. They are engineered for the transduction of nearly all mammalian cells, including difficult-to-transfect cells. To ensure the highest possible titer and quality, Lentifect™ lentivirus is produced from standardized protocols, with stringent quality control processes, which include sequence verification of the insert and qRT-PCR based titer estimation.
    View transduction results of H1299 cells with Lentifect eGFP or mCherry lentivirus.
    Advantages
    • Convenience. Ready for transduction upon delivery.
    • High titer. ≥ 108 TU/ml, providing efficient transduction of virtually all mammalian cell types.
    • Versatility. Lentifect™ lentiviral particles can express nearly any GeneCopoeia clone type, including ORF cDNA (untagged or tagged), shRNA, sgRNA, miRNA and promoters.
    • Selection. Available in vectors with a wide variety of promoters, selection markers, and reporters.
     
    NEW! GeneCopoeia also provides Lentifect™ Ultra-purified Lentivirus, which is subjected to further purification necessary for use in animal models.

    Search all lentivirus products by gene ID or accession number

    Custom Lentivirus

    Lentifect™ Custom Lentivirus

    Lentifect™ custom lentivirus production services are available for nearly any GeneCopoeia clone type, including:
    Lenti-ORF cDNA
    Choose GeneCopoeia’s transduction-ready lentivirus delivering ORF cDNA, with over 80 plasmids carrying a variety of combinations of promoter, selection marker and reporter gene.
    Lentiviral ORF cDNA Plasmids
    Promoter Selection Marker Reporter/Tag
    CMV, EF1a, PGK, CAG, UBC Puromycin, neomycin, hygromycin, blasticidin. GFP, mCherry, YFP, CFP; His, flag, HA, myc, avi, halo, V5, luciferase.
    Lenti-CRISPR
    Genome integration of CRISPR elements using lentivirus. Cas9 or sgRNA packed in purified lentiviral particles at 108 TU/ml, ready to infect all cell types.

         

    Custom sgRNA Lentiviral Particles

    Vector Promoter sgRNA and Cas9 Selection Marker/ Reporter Gene Vector Type Vector Map
    pCRISPR-LvSG03 U6 sgRNA only Puromycin / mCherry Lentiviral get_info

    Pre-made sgRNA Control Particles

    Buy Catalog# Product Description Vector Price
    Pre-made purified sgRNA control lentiviral particles
    LP506-025 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 25 µL (U6/T7/mCherry/Puro) pCRISPR-LvSG03 $295
    LP506-100 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 100 µL (U6/T7/mCherry/Puro) pCRISPR-LvSG03 $725

    Pre-made Cas9 Nuclease Lentiviral Particles

    Buy Catalog Product Name Description Price
    LP601-100 Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) ≥1x108 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (CMV/Neomycin) $725
    LP602-100 Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) 1x106 - 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (CMV/eGFP/Neomycin) $725
    LP603-100 Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) ≥1x108 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/Puromycin) $725
    LP604-100 Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) ≥1x108 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/eGFP/Neomycin) $725
    LP605-100 Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) ≥1x108 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/eGFP/Hygromycin) $725
    Lenti-shRNA
    Transduction-ready lentivirus delivering shRNA of varying lengths (19 to 29 bases) that were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect.

    Promoters of your choice

    shRNA driven by pol III promoters (H1, U6) or pol II promoters (CMV, EF1a, CAG and CBh), both with great knockdown efficiency. Request a quote for pol II promoter-driven shRNA lentivirus or AAV services. pol III promoter-driven shRNA pol II promoter-driven shRNA

    Vector Promoter Selection Marker Reporter Gene Viral type
    psi-LVRH1GP H1 Puromycin eGFP HIV
    psi-LVRU6GP U6 Puromycin eGFP HIV
    psi-LVRH1MP H1 Puromycin mCherry HIV
    psi-LVRU6MP U6 Puromycin mCherry HIV
    psi-LVRH1P H1 Puromycin N/A HIV
    psi-LVRU6P U6 Puromycin N/A HIV
    psi-LVRU6GH U6 Hygromycin EGFP HIV
    psi-LVRH1GH H1 Hygromycin EGFP HIV
    psi-LVRU6MH U6 Hygromycin mCherry HIV
    psi-LVRH1MH H1 Hygromycin mCherry HIV
    psi-LVRU6H U6 Hygromycin N/A HIV
    psi-LVRH1H H1 Hygromycin N/A HIV
    psi-LVE001 CMV Puromycin eGFP HIV
    psi-LVE002 CMV Puromycin mCherry HIV
    psi-LVE003 EF1a Puromycin eGFP HIV
    psi-LVE004 EF1a Puromycin mCherry HIV

    Request a quote for pol II promoter-driven shRNA lentivirus services.

    Lenti-Promoter
    GeneCopoeia’s promoter lentivirus make your studies in gene regulation easier! Evaluate the performance of your promoter by the activation of reporter gene expression, including Gaussia luciferase, GFP and mCherry.

    Lentiviral Promoter Plasmids
    Reporter Gene Tracking Gene Selection Marker
    Gaussia luciferase, eGFP, mCherry, td Tomato Secreted alkaline phosphatase (SEAP), N/A Puromycin
    To order custom Lentifect™ purified lentiviral particles visit our search page.

    Lentiviral vectors are potent vehicles for delivery of genes into a wide range of cell types including difficult-to-transfect cells. Lentiviral particles transduce cells and integrate into the host genome in dividing and post-mitotic cells resulting in long-term expression of the transgene in vitro. Producing, concentrating and titrating lentiviral particles, however, is time consuming and requires some experience to achieve high titers and consistent results. GeneCopoeia’s Lentifect™ purified lentiviral particles save you time and effort, and provide high-level expression of genetic elements of interest (Figure 1).

    Transduction of H1299 cells with GeneCopoeia’s Lentifect™ lentiviral particles expressing eGFP.

    Figure 1. H1299 cells in 24-well plates were transduced with the indicated amounts of eGFP purified particles, (catalog number LP303) in the presence of 5 µg/ml of polybrene. The expression of eGFP was visualized with a fluorescence microscope 72 hours post-transduction.

    Transduction of H1299 cells with GeneCopoeia’s Lentifect™ lentiviral particles expressing mCherry.
    Figure 2. H1299 cells in 24-well plates were transduced with the indicated amounts of mCherry purified particles, (catalog number LP151) in the presence of 5 µg/ml of polybrene. The expression of mCherry was visualized with a fluorescence microscope 72 hours post-transduction. </p > * Promotion is valid in the US & Canada only. For international customers, please contact your local distributors. Discounts on lentivirus are valid on premade lentiviral particles and standard-catalog LPP or ULP custom lentiviral particles. Discounts are not valid on particles that require gene synthesis or custom modification services. Discounts are not valid on previous purchases and cannot be combined with additional discounts.