Biotin Protein Ligase

Biotin Protein Ligase
Biotin Protein Ligase

Biotin Protein Ligase

Price: $250.00
  • BI001
Size: 300,000 Units
Manual: Download
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Biotin-protein ligase (EC activates biotin to form biotinyl 5′ adenylate and transfers the biotin to biotin-accepting proteins. It also functions as a biotin operon repressor. The protein is encoded by the birA gene. Other names for this enzyme include: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase.

Source: E. coli

Storage buffer: 50 mM imidazole, pH 6.8, 50 mM NaCl, 5% glycerol, 5 mM mercaptoethanol

Storage conditions: The enzyme arrives on dry ice and should be immediately stored at -80°C. After the vial is thawed it should be stored at 4°C if it is to be re-used in the near future. For longer term storage a vial of thawed enzyme can be safely re-frozen by dropping into liquid nitrogen before storing at -80°C. Biomix A, B, and d-biotin can be stored at -20°C. There is no problem in thawing and re-freezing these mixtures.

Stability: Biotin ligase retains >90% of its activity for >3 months when stored at 4°C

Concentration: 1.0 mg/mL by A280

Purity: >98% by Coomassie staining

Activity: 5,000 Units/µg

Definition of Activity: 1 Unit is the amount of enzyme that will biotinylate 1 pmol of peptide substrate* in 30 minutes at 30°C using the reaction buffers provided and 38 µM peptide substrate*.

* The peptide substrate used in the enzyme assays was a 15-mer variant of sequence #85 identified by Schatz (1).

Contaminating proteases: <0.01% as chymotrypsin-like activity